Plant Gene Isolation
Principles and Practice
Wiley (Publisher)
Published on 11. April 1996
Book
Paperback/Softback
448 pages
978-0-471-95539-9 (ISBN)
Description
Gene isolation has become an essential step in dissecting the molecular control of fundamental plant processes, enabling their manipulation and improvement by genetic engineering. However, the isolation of the "gene of interest" often represents a formidable task. Many approaches are well established, but new approaches and techniques have been developed in recent years which can streamline the progress of a gene cloning project. This volume provides a comprehensive guide to the history, theoretical background and application of the experimental methods used to undertake the isolation of genes from plants. Laboratory procedures are described, and advice on which technique to use is given. Information on useful sources and suppliers is also featured.
More details
Language
English
Place of publication
Chichester
United Kingdom
Publishing group
John Wiley and Sons Ltd
Target group
College/higher education
Professional and scholarly
Illustrations
Illustrations
Dimensions
Height: 241 mm
Width: 165 mm
Weight
810 gr
ISBN-13
978-0-471-95539-9 (9780471955399)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Classification
Content
Plant Genome Constituents and Their Organization; LIBRARY CONSTRUCTION: cDNA Library Construction; Genomic Library Construction; BAC, YAC and Cosmid Library Construction; LIBRARY SCREENING: Heterologous and Homologous Gene Probes; Differential Screening; Expression Library Screening; Functional Complementation in Yeast and E. coli; MAP-BASED CLONING: Classical Mutagenesis and Gene Mapping; Chromosome Walking; INSERTIONAL MUTAGENESIS: T-DNA Mediated Insertional Mutagenesis; Transposon Mediated Insertional Mutagenesis; PCR-BASED CLONING: PCR Techniques; cDNA and Genomic Subtraction; SEQUENCING PROJECTS: EST and Genomic Sequencing Projects.