Methods in Cellular Immunology
1st Edition
Published on 27. June 1995
Book
Paperback/Softback
250 pages
978-0-8493-6548-5 (ISBN)
Description
Methods in Cellular Immunology provides a guide to cellular immunological methods. This indispensible book covers both murine and human models. The described methods involve cell isolation and separation; antibody production, detection, and isolation; flow cytometry; NK cytotoxicity; and quantitation of cytokines and cytokine receptors.This practical, easy-to-follow methods book presents step-by-step descriptions of the most common techniques of cellular immunology in a style that both novices and seasoned scientists will be able to understand. Text includes the background information necessary to perform basic techniques. An extensive list of references is provided, allowing readers to compare the methods in the book with the original techniques. The book also lists the manufacturers of all chemicals and materials used in the procedures.
Both scientists and researchers involved in basic and clinical research and all scientists wishing to employ immunological techniques will benefit from this valuable resource.
Both scientists and researchers involved in basic and clinical research and all scientists wishing to employ immunological techniques will benefit from this valuable resource.
More details
Language
English
Place of publication
Bosa Roca
United States
Publishing group
Taylor & Francis Inc
Target group
College/higher education
Professional and scholarly
Cell biologists, immunologists, and microbiologists.
Illustrations
6 s/w Tabellen
6 tabs.
Dimensions
Height: 235 mm
Width: 156 mm
Weight
365 gr
ISBN-13
978-0-8493-6548-5 (9780849365485)
Schweitzer Classification
Other editions
New editions
Rafael Fernandez-Botran
Methods in Cellular Immunology
Book
06/2001
2nd Edition
CRC Press
€252.90
Shipment within 15-20 days
Content
Cells
Preparation of Cell Suspensions
Isolation of Cells
References
Flow Cytometry
Proliferation Assays
Incorporation of Tritiated Thymidine into DNA
Nonradioactive Detection Methods (MTT Assay)
References
Preparation of Cell Clones
Preparation of Mouse T Cell Clones
Preparation of Human T Cell Clones
Preparation of Human B Cell Clone
Cloning by Limiting Dilution Technique
References
Cytotoxic Cells
Cell-Mediated Cytotoxicity
References
Assays of Cytokines
Introduction
Bioassays
Immunoassays
References
Cytokine Receptors
Preparation and Modification of Antibodies
Polyclonal Antibodies
Monoclonal Antibodies
Coupling of Synthetic Peptides to Carrier Proteins
References
Detection of Antibodies
Hemolytic Plaque Assays
ELISA
References
Purification of Immunoglobulins
Precipitation by Ammonium Sulfate
Caprylic Acid-Ammonium Sulfate Isolation of IgG from Ascites Fluid
Purification of Antibodies by Precipitation with Polyethylene Glycol
Purification of Antibodies from Culture Supernatants by Precipitation with Polyethylene Glycol
Affinity Chromatography Using Protein A-Sepharose
Purification of IgM
Ammonium Sulfate Precipitation of IgM
Purification of Anti-Hapten Antibodies by Affinity Chromatography
Fragmentation of Immunoglobulin into F(ab) Fragments using Papain
Fragmentation of Immunoglobulin into F(ab) Fragments using Preactivated Papain
Fragmentation of Immunoglobulin into F(ab)2 Fragments using Pepsin
Double Immunodiffusion Assay
References
Buffers and Media
Commercial Sources
Index
Preparation of Cell Suspensions
Isolation of Cells
References
Flow Cytometry
Proliferation Assays
Incorporation of Tritiated Thymidine into DNA
Nonradioactive Detection Methods (MTT Assay)
References
Preparation of Cell Clones
Preparation of Mouse T Cell Clones
Preparation of Human T Cell Clones
Preparation of Human B Cell Clone
Cloning by Limiting Dilution Technique
References
Cytotoxic Cells
Cell-Mediated Cytotoxicity
References
Assays of Cytokines
Introduction
Bioassays
Immunoassays
References
Cytokine Receptors
Preparation and Modification of Antibodies
Polyclonal Antibodies
Monoclonal Antibodies
Coupling of Synthetic Peptides to Carrier Proteins
References
Detection of Antibodies
Hemolytic Plaque Assays
ELISA
References
Purification of Immunoglobulins
Precipitation by Ammonium Sulfate
Caprylic Acid-Ammonium Sulfate Isolation of IgG from Ascites Fluid
Purification of Antibodies by Precipitation with Polyethylene Glycol
Purification of Antibodies from Culture Supernatants by Precipitation with Polyethylene Glycol
Affinity Chromatography Using Protein A-Sepharose
Purification of IgM
Ammonium Sulfate Precipitation of IgM
Purification of Anti-Hapten Antibodies by Affinity Chromatography
Fragmentation of Immunoglobulin into F(ab) Fragments using Papain
Fragmentation of Immunoglobulin into F(ab) Fragments using Preactivated Papain
Fragmentation of Immunoglobulin into F(ab)2 Fragments using Pepsin
Double Immunodiffusion Assay
References
Buffers and Media
Commercial Sources
Index