Introduction to Fluorescence Correlation Spectroscopy

Fluorescence correlation spectroscopy (FCS) uses measurements of fluorescent molecules to determine chemical reaction rates and other kinetic parameters. This book introduces theoretical and practical aspects of FCS, including use in basic research, biomedicine, and biotechnology. State-of-the-art applications discussed include high-throughput molecular screening by pharmaceutical companies and studies of a wide range of molecular processes. The author, a world-recognized expert, provides detailed discussion of methodology, amplitude approaches, interpretation, photobleaching, physical and biological science applications, single molecule studies, and future directions.