Real-Time PCR
An Essential Guide
Published on 15. April 2004
Book
Hardback
300 pages
978-0-9545232-7-5 (ISBN)
Description
This essential manual presents a comprehensive guide to the most appropriate and up-to-date technologies and applications as well as providing an overview of the theory of this important technique. Written by recognized experts in the field this timely and authoritative volume is an essential requirement for all laboratories using PCR. Topics covered include: Real-time PCR instruments and probe chemistries, set-up, controls and validation, quantitative real-time PCR, analysis of mRNA expression, mutation detection, NASBA, application in clinical microbiology and diagnosis of infection.
More details
Language
English
Place of publication
London
United Kingdom
Target group
Professional and scholarly
College/higher education
Illustrations
28 s/w Abbildungen, 22 s/w Tabellen
22 Tables, black and white; 28 Illustrations, black and white
Dimensions
Height: 229 mm
Width: 152 mm
Weight
652 gr
ISBN-13
978-0-9545232-7-5 (9780954523275)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Persons
Health Protection Agency, UK
Health Protection Agency, UK
Health Protection Agency, UK
Health Protection Agency, UK
Health Protection Agency, UK
Content
1. An Introduction to Real-Time PCR 2. An Overview of Real-Time PCR Platforms 3. Homogeneous Fluorescent Chemistries for Real-Time PCR 4. Performing Real-Time PCR 5. Internal and External Controls for Reagent Validation 6. Quantitative Real-Time PCR 7. Analysis of mRNA Expression by Real-Time PCR 8. Mutation Detection by Real-Time PCR 9. The Quantitative Amplification Refractory Mutation System 10. Real-Time NASBA 11. Applications of Real-Time PCR in Clinical Microbiology 12. Application of Real-Time PCR to the Diagnosis of Invasive Fungal Infection