Expression Systems

Protein expression is an increasingly important tool for research on gene function. What is needed is not just a lab manual providing established methods as well as the latest state-of-the-art protocols, but also clear advice on what expression system to choose when.

Expression Systems: Methods Express uniquely fills this need. It covers expression across a broad range of systems, including the following:






Baculovirus expression vectors
CHO cells
E. coli
HEK293-EBNA1 cells
Lactococcus lactis and other gram positive bacteria
S. cerevisiae
Transfected insect cells
Pichia pastoris
Mammalian cells using BacMam viruses
Lentiviral vectors
Wheat germ cell-free system


The book takes the reader through how to make an informed choice of appropriate system, taking into account the protein target, the time involved, the ultimate use of the expressed protein, and the laboratory equipment required. It also provides step-by-step methods for each system. In addition, the book describes the optimisation of expression strategies, expression engineering using ribosome display, and how to select protein variants with improved expression.

Every chapter discusses the merits and limitations of the approaches available, describes the key techniques in full practical detail, and provides sensible advice for immediate use at the bench.

In summary, Expression Systems: Methods Express is a comprehensive laboratory manual and information resource for researchers at all levels, from postgraduate student to principal investigator.