Crystallization of Nucleic Acids and Proteins
2nd Edition
Published on 1. January 2000
Book
Hardback
304 pages
978-0-19-963679-2 (ISBN)
Description
Crystallography is the major method of determining structures of biological macromolecules, yet crystallization techniques are still regarded as difficult to perform. This text continues in the vein of the first edition by providing a detailed and rational guide to producing crystals of proteins and nucleic acids of sufficient quantity and quality for diffraction studies. It has been thoroughly updated to include all the major new techniques such as the uses of molecular biology in structural biology (maximizing expression systems, sequence modifications to enable crystallization, and the introduction of anomalous scatterers); diagnostic analysis of prenucleation and nucleation by spectroscopic methods; and the two-dimensional electron crystallography of soluble proteins on planar lipid films.
More details
Edition
2nd Revised edition
Language
English
Place of publication
Oxford
United Kingdom
Target group
Professional and scholarly
Edition type
Revised edition
Illustrations
index
ISBN-13
978-0-19-963679-2 (9780199636792)
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Schweitzer Classification
Other editions
Previous edition
Arnaud Ducruix
Crystallization of Nucleic Acids and Proteins
Book
02/1992
Oxford University Press
€34.05
Article exhausted; check for reprint
Persons
Content
Giege and Ducruix: An introduction to the crystallogenesis of biological macromolecules; Lorber and Giege: Biochemical aspects and handling of macromolecular solutions and crystals; Berne, Doublie and Carter, Jr: Molecular biology for structural biology; Carter, Jr: Experimental design, quantitive analysis, and the cartography of crystal growth; Ducruix and Giege: Methods of crystallization; Robert et al: Crystallization in gels and related methods; Stura: Seeding techniques; Dock-Bregeon, Moras and Giege: Nucleic acids and their complexes; Reiss-Husson and Picot: Crystallization of membrane proteins; Ries-Kaut and Ducruix: From solution to crystals with a physico-chemical aspect; Veesler and Boistelle: Diagnostic of prenucleation and nucleation by spectroscopic methods and background on the physics; Brisson, Lambert and Bergsma-Schutter: Two-dimensional crystallization of soluble proteins on planar lipid films; Stura and Gleichmann: Soaking techniques; Sawyer and Turner: X-ray analysis.