Crystallization of Nucleic Acids and Proteins: A Practical Approach
Published on 27. February 1992
Book
Paperback/Softback
360 pages
978-0-19-963246-6 (ISBN)
Description
In the last decade crystallography has emerged as a key tool for understanding the structures of biological macromolecules, yet the crystallization techniques themselves have been perceived as difficult to perform. This book provides the first detailed and rational guide to producing crystals of proteins and nucleic acids for diffraction studies. Standard crystallization methods are given, along with procedures for the preparation and handling of macromolecules.
Detailed laboratory protocols and the underlying theoretical background are brought together, providing researchers with the conceptual and methodological tools necessary to exploit these powerful techniques.
Detailed laboratory protocols and the underlying theoretical background are brought together, providing researchers with the conceptual and methodological tools necessary to exploit these powerful techniques.
Reviews / Votes
'an invaluable contribution to the literature in this research area ... it will be most useful for students and beginners in the field of crystallization ... this very up-to-date, comprehensive text ... is written and presented with great clarity. I can strongly recommend it as a standard text on crystallization that no laboratory involved in the field should be without.'Lesley F. Lloyd, Imperial College of Science, Technology and Medicine, Tibtech, April 1993 (Vol. 11) 'a work that is both a laboratory manual and a treatise on the physics of crystallization ... this is a modern laboratory manual useful for anyone working in this research area ... Its value to the experimentalist lies in the presentation of techniques and, probably more importantly, in the discussions of crystallization theory.'
Patricia C. Weber, The Du Pont Merck Pharmaceutical Co. Acta Cryst. (1994)
More details
Series
Language
English
Place of publication
Oxford
United Kingdom
Target group
College/higher education
Professional and scholarly
Illustrations
halftones, line illustrations, tables
Dimensions
Height: 234 mm
Width: 155 mm
Thickness: 20 mm
Weight
548 gr
ISBN-13
978-0-19-963246-6 (9780199632466)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
New editions
Book
10/1999
2nd Edition
Oxford University Press
€95.32
Shipment within 15-20 days
Persons
Content
R. Giege & A. Ducruix: An introduction to the crystallogenesis of biological macromolecules; B. Lorber & R. Giege: Preparation and handling of biological macromolecules for crystallization; C.W. Carter: Design of crystallization experiments and protocols; A. Ducruix & R. Giege: Methods of crystallization; E. Stura & I.A. Wilson: Seeding techniques; M.C. Robert, K. Provost, & F. Lefaucheux: Crystallization in gels and related
methods; A.C. Dock-Bregeon & D. Moras: Crystallization of nucleic acids and co-crystallization of proteins and nucleic acids; F. Reiss-Husson: Crystallization of membrane proteins; M. Ries-Kaut & A. Ducruix: Phase diagrams; V. Mikol & R. Giege: The physical chemistry of protein crystallization; E.A. Stura & P. Chen: Soaking of crystals; L.
Sawyer & M.A. Turner: X-ray analysis; K.B. Ward, M.A. Perozzo & W.M. Zuk: Automating crystallization experiments; S. Doublie & C.W. Carter: Preparation of selenomethionyl protein crystals; Appendix; Index.
methods; A.C. Dock-Bregeon & D. Moras: Crystallization of nucleic acids and co-crystallization of proteins and nucleic acids; F. Reiss-Husson: Crystallization of membrane proteins; M. Ries-Kaut & A. Ducruix: Phase diagrams; V. Mikol & R. Giege: The physical chemistry of protein crystallization; E.A. Stura & P. Chen: Soaking of crystals; L.
Sawyer & M.A. Turner: X-ray analysis; K.B. Ward, M.A. Perozzo & W.M. Zuk: Automating crystallization experiments; S. Doublie & C.W. Carter: Preparation of selenomethionyl protein crystals; Appendix; Index.