Experiments in Plant Tissue Culture
3rd Edition
Published on 27. January 1995
Book
Hardback
272 pages
978-0-521-47313-2 (ISBN)
Description
In this third edition the authors have written a guide to the essentials of plant tissue culture. The book takes the reader through a graded series of experimental protocols and provides an introductory review of each topic. There are discussions of aseptic techniques, and nutritional components of media. Subsequent chapters cover such topics as callus formation, organogenesis, xylem cell differentiation, root cultures, cell suspensions, micropropagation by buds, somatic embryogenesis, protoplast culture and fusion, secondary metabolite production, and quantitation of procedures, amongst others. A glossary, commercial sources of supplies, and the formulations of culture media are also included. This new edition has been completely revised and updated. The organization has been improved, and new illustrations added, together with new experiments on such topics as potato callus formation, and embryo culture.
Reviews / Votes
'This well thought out book does considerably more than is claimed in the title. It not only takes the reader through a graded series of experimental protocols covering the major techniques used in plant tissue culture, it also provides a good introductory review of each topic.' Trends in Biochemical Sciences '... the authors have written ... a text based on their extensive experience ... directed in the first instance towards students, these topics will prove [to be] of immense value to a much wider range of readers, whatever their previous knowledge.' Cellular and Molecular Biology '... a worthy text and an excellent addition to any plant science laboratory.' John Love, British Society for Developmental Biology NewsletterMore details
Edition
3rd Revised edition
Language
English
Place of publication
Cambridge
United Kingdom
Target group
College/higher education
Edition type
Revised edition
Illustrations
7 Tables, unspecified; 15 Halftones, unspecified; 10 Line drawings, unspecified
Dimensions
Height: 237 mm
Width: 157 mm
Thickness: 18 mm
Weight
520 gr
ISBN-13
978-0-521-47313-2 (9780521473132)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
New editions
Book
01/1995
3rd Edition
Cambridge University Press
€83.10
Shipment within 15-20 days
Additional editions
Book
01/1995
3rd Edition
Cambridge University Press
€83.10
Shipment within 15-20 days
Persons
Content
Part I. Background Information: 1. Culture of plant cells, tissues, and organs; 2. Laboratory facilities; 3. Aseptic techniques; 4. Media composition and preparation; Part II. Experimental: Callus and Callus-Derived Systems: 5. Initiation and maintenance of callus; 6. Organogenesis; 7. Cell suspensions; 8. Somatic embryogenesis; Part III. Experimental: Culture of Organs and Organized Systems: 9. Isolated roots; 10. Micropropagation by bud proliferation; 11. Anther and pollen cultures; Part IV. Experimental: Isolated Cells: 12. Transdifferentiation of parenchyma cells to tracheary elements; 13. Isolation and culture of protoplasts; 14. Protoplast fusion and somatic hybridization; Part V. Supplementary Topics: 15. Cryopreservation of germplasm; 16. Production of secondary metabolites; 17. Quantitation of procedures; 18. Formulations of tissue culture media; 19. Commercial sources of supplies; Author index; Subject index.