Guide to Protein Purification
Volume 182: Guide to Protein Purification
Murray P. Deutscher(Editor)
Academic Press
Published on 28. February 1990
Book
Paperback/Softback
894 pages
978-0-12-213585-9 (ISBN)
Description
"The Guide to Protein Purification" is a self-contained volume covering all the important procedures for purifying proteins, as well as other more specialized techniques. However, to stay within the confines of a single volume, some details are dealt with by reference to other works, but these have been kept to a minimum. It is hoped that this volume will satisfy the needs of both the novice in protein purification and the more experienced researcher. It contains procedures newly written for this volume; and provides extensive practical information. It clearly describes rationale and strategies for protein and enzyme purification. It offers personal perspectives on enzyme purification by eminent researchers.
Reviews / Votes
"In summary, this is possibly the most important, comprehensive and affordable work on protein purification to have appeared in recent years." TIB-TECHMore details
Series
Language
English
Place of publication
San Diego
United States
Publishing group
Elsevier Science Publishing Co Inc
Target group
Professional and scholarly
Dimensions
Height: 241 mm
Width: 178 mm
Weight
1262 gr
ISBN-13
978-0-12-213585-9 (9780122135859)
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Schweitzer Classification
Content
Developing Purification Procedures. General Methods for Handling Proteins and Enzymes. Optimization of Starting Materials. Preparation of Extracts and Subcellular Fractionation. Solubilization Procedures. Purification Procedures: Bulk Methods. Purification Procedures: Chromatographic Methods. Purification Procedures: Electrophoretic Methods. Specialized Purification Procedures. Characterization of Purified Proteins. Immunological Procedures. Additional Techniques. Concluding Remarks. Personal Perspectives on Enzyme Purification. Index.