Molecular Protocols in Transfusion Medicine
Published on 27. April 2000
Book
Paperback/Softback
186 pages
978-0-12-209370-8 (ISBN)
Description
This essential methods manual for immunohematologists (or hematologists and immunohematologists) provides information on genes that encode antigens on red blood cells, platelets and neutrophils. The book begins by covering general concepts in molecular biology and specific protocols such as DNA preparation, PCR-RFLP and allele-specific PCR. Information on the erythrocyte, platelet and neutrophil antigen systems and the molecular basis of polymorphisms are presented clearly in a gene facts sheet format. Database accession numbers and useful adjuncts such as Request forms, worksheets for PCR/enzyme digests also serve to benefit the user. The information is clearly presented and easily accessible and is complemented by the excellent diagrams and tabular material. This book is invaluable for both new and experienced researchers in the field and other related disciplines.
More details
Language
English
Place of publication
San Diego
United States
Publishing group
Elsevier Science Publishing Co Inc
Target group
Professional and scholarly
Haematologists, blood bank technologists and immunologists
Product notice
Paperback (trade)
Unsewn / adhesive bound
Dimensions
Height: 235 mm
Width: 171 mm
Thickness: 14 mm
Weight
358 gr
ISBN-13
978-0-12-209370-8 (9780122093708)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Persons
Content
Preface
List of Abbreviations
Part 1: Introduction
1.1 Background
1.2 Aim, Scope and Purpose of the Book
1.3 Organization of the Book
1.4 Terminology
1.5 Regulatory Compliance
1.6 References
Part 2: General Considerations
2.1 Clinical Information
2.2 Basic Principles of Molecular Analysis
2.3 Troubleshooting
2.4 References
Part 3: Protocols
3.1 Sample Shipment and Storage
3.2 Reagents
3.3 Harvesting WBCs by RBC Sedimentation
3.4 Short-term Cell Cultures from Amniotic Fluid
3.5 Cultured Amniocyte Cell Harvesting
3.6 Cell Preparation and DNA Extraction
3.7 Assessment of DNA Quality, Quantity and Purity
3.8 PCR Amplification
3.9 Restriction Enzyme Digestion for PCR-RFLP Analysis
3.10 Allele-specific PCR
3.11 Agarose Gel Preparation and Electrophoresis
3.12 Acrylamide Gel Preparation and Electrophoresis
3.13 Single-strand Conformation Polymorphism (SSCP)
3.14 Optimizing a PCR
3.15 Gene Zygosity Assessment by Quantitative PCR Amplification
3.16 VNTR and STR Analysis by PCR Amplification
3.17 Multiplex PCR Analysis
3.18 Southern Analysis
3.19 References
Part 4: Red Cell Blood Groups
4.1 Terminology
4.2 Clinical Applications
4.3 Situations where Genotype and Phenotype do not Agree
4.4 References
4.5 Facts Sheets, Gene Maps and Molecular Protocols
Part 5: Platelet Blood Groups
5.1 Terminology
5.2 Clinical Applications
5.3 References
5.4 Facts Sheets, Gene Maps and Molecular Protocols
Part 6: Neutrophil Blood Groups
6.1 Terminology
6.2 Clinical Applications
6.3 References
6.4 Facts Sheets, Gene Maps and Molecular Protocols
Part 7: Appendices
7.1 Database Information
7.2 Glossary
7.3 References
7.4 Works Sheets and Request Forms
Index
Useful Tables
List of Abbreviations
Part 1: Introduction
1.1 Background
1.2 Aim, Scope and Purpose of the Book
1.3 Organization of the Book
1.4 Terminology
1.5 Regulatory Compliance
1.6 References
Part 2: General Considerations
2.1 Clinical Information
2.2 Basic Principles of Molecular Analysis
2.3 Troubleshooting
2.4 References
Part 3: Protocols
3.1 Sample Shipment and Storage
3.2 Reagents
3.3 Harvesting WBCs by RBC Sedimentation
3.4 Short-term Cell Cultures from Amniotic Fluid
3.5 Cultured Amniocyte Cell Harvesting
3.6 Cell Preparation and DNA Extraction
3.7 Assessment of DNA Quality, Quantity and Purity
3.8 PCR Amplification
3.9 Restriction Enzyme Digestion for PCR-RFLP Analysis
3.10 Allele-specific PCR
3.11 Agarose Gel Preparation and Electrophoresis
3.12 Acrylamide Gel Preparation and Electrophoresis
3.13 Single-strand Conformation Polymorphism (SSCP)
3.14 Optimizing a PCR
3.15 Gene Zygosity Assessment by Quantitative PCR Amplification
3.16 VNTR and STR Analysis by PCR Amplification
3.17 Multiplex PCR Analysis
3.18 Southern Analysis
3.19 References
Part 4: Red Cell Blood Groups
4.1 Terminology
4.2 Clinical Applications
4.3 Situations where Genotype and Phenotype do not Agree
4.4 References
4.5 Facts Sheets, Gene Maps and Molecular Protocols
Part 5: Platelet Blood Groups
5.1 Terminology
5.2 Clinical Applications
5.3 References
5.4 Facts Sheets, Gene Maps and Molecular Protocols
Part 6: Neutrophil Blood Groups
6.1 Terminology
6.2 Clinical Applications
6.3 References
6.4 Facts Sheets, Gene Maps and Molecular Protocols
Part 7: Appendices
7.1 Database Information
7.2 Glossary
7.3 References
7.4 Works Sheets and Request Forms
Index
Useful Tables