Description
Animal Cell Culture: Methods Express is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.
More details
Series
Language
English
Place of publication
Bloxham
United Kingdom
Target group
Professional and scholarly
Dimensions
Height: 244 mm
Width: 172 mm
Thickness: 13 mm
ISBN-13
978-1-904842-71-2 (9781904842712)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Classification
Content
1. The cell culture laboratory Susan Clarke, ImmunoBiology Ltd 2. Sterilization Peter Roberts, Bio-Products Laboratory 3. Development and optimization of animal cell culture media Stephen Gorfien, Invitrogen Corporation, and David Jayme, Brigham-Young University, Hawaii 4. Microscopy of living cells Daniel Zicha and Colin Gray, both at Cancer Research UK London Research Institute 5. Basic techniques, the maintenance of cell lines, and safety John Davis, University of Hertfordshire 6. Cryopreservation and cell banking Glyn Stacey, UK Stem Cell Bank, National Institute for Biological Standards and Control 7. Primary culture and the establishment of new cell lines Jan-Thorsten Schantz, National University of Singapore 8. Specific cell types and their requirements Chris Morris, Health Protection Agency 9. Cloning John Clarke, St Thomas' Medical School; Alison Porter, Lonza Biologics plc; and John Davis, University of Hertfordshire 10. The quality control of cell lines and the prevention, detection and cure of contamination Cathy Rowe and Peter Thraves, both at the Health Protection Agency 11. Viability and cytotoxicity assays Sandra Coecke, European Centre for the Validation of Alternative Methods 12. Systems for culture scale-up John Davis, University of Hertfordshire 13. Good laboratory practice (GLP) in the cell culture laboratory Barbara Orton, Bio-Products Laboratory List of suppliers Index