Essential Cytometry Methods
Published on 27. November 2009
Book
Paperback/Softback
920 pages
978-0-12-375045-7 (ISBN)
Description
Cytometry is characterization and measurement of cells and cellular constituents, most often used to immunophenotype cells - that is, to distinguish healthy cells from diseased cells. Flow Cytometry specifically is quite sensitive, allowing researchers to detect rare cell types and residual levels of disease, and as such has been the method of choice for important studies such as monitoring the blood of AIDS patients. For this reason, there is a great need for a practical, comprehensive manual that will be useful across a broad range of laboratories. This volume, as part of the Reliable Lab Solution Series, delivers such a tool, offering busy researchers across many disciplines a handy resource of all the best methods and protocols for Cytometry to use at the bench.
More details
Series
Language
English
Place of publication
San Diego
United States
Publishing group
Elsevier Science Publishing Co Inc
Target group
College/higher education
Professional and scholarly
Researchers in and students of biochemistry, molecular and cell biology, developmental biology, and genetics.
Product notice
Paperback (trade)
Unsewn / adhesive bound
Dimensions
Height: 234 mm
Width: 192 mm
Thickness: 37 mm
Weight
1392 gr
ISBN-13
978-0-12-375045-7 (9780123750457)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
Additional editions
Zbigniew Darzynkiewicz | J. Paul Robinson | Mario Roederer
Essential Cytometry Methods
E-Book
05/2014
Academic Press
€62.95
Available for download
Persons
Purdue University, W. Lafayette, IN, USA
Editor
CANCER RESEARCH INSTITUTE OF THE
NEW YORK MEDICAL COLLEGE AT VALHALLA
NEW YORK MEDICAL COLLEGE AT VALHALLA
Director of the Purdue University Cytometry Laboratories and Professor of Basic Medical Science and Biomedical Engineering, Purdue University, IN, USA.
Immuno-Technology Section and Flow Cytometry Core, Vaccine Research Center, NIAD, NIH, Bethesda, MD, U.S.A.
Content
Part I: Fluorochromes/General Techniques
1. Principles of confocal microscopy
(Robinson)
2. Protein labeling with fluorescent probes
(Holmes, Lantz)
3. Cytometry of fluorescence resonance energy transfer
(Vereb, Matko, Szollosi)
4. The rainbow of fluorescent proteins
(Galbraith)
5. Labeling cellular targets with semiconductor quantum dot conjugates
(Wu, Bruchez)
6. High-gradient magnetic cell sorting
(Radbruch, Mechtold, Thiel, Miltenyi, Pfluger)
7. Multiplexed microsphere assays for protein and DNA binding reactions
(Kellar, Oliver)
8. Biohazard sorting
(Schmid, Roederer, Koup, Ambrozak, Perfetto)
9. Guidelines for the presentation of flow cytometric data.
(Roederer, Darzynkiewicz, Paks)
Part II: Cellular DNA Content Analysis
10. VinDetergent and proteolytic enzyme-based techniques for nuclear isolation and DNA content analysis
(Vindelov,Christensen)
11. Rapid DNA content analysis
(Krishan)
12. DNA analysis from paraffin-embedded blocks
(Hedley)
13. Flow cytometry and sorting of plant protoplasts and cells
(Galbraith)
14. DNA content histogram and cell cycle analysis.
(Rabinovitch)
15. Simultaneous analysis of cellular RNA and DNA content
(Darzynkiewicz)
Part III. Cell Proliferation and Death Assays
16. Immunochemical quantitation of bromodeoxyuridine: application to cell cycle kinetics
(Dolbeare, Selden)
1. Principles of confocal microscopy
(Robinson)
2. Protein labeling with fluorescent probes
(Holmes, Lantz)
3. Cytometry of fluorescence resonance energy transfer
(Vereb, Matko, Szollosi)
4. The rainbow of fluorescent proteins
(Galbraith)
5. Labeling cellular targets with semiconductor quantum dot conjugates
(Wu, Bruchez)
6. High-gradient magnetic cell sorting
(Radbruch, Mechtold, Thiel, Miltenyi, Pfluger)
7. Multiplexed microsphere assays for protein and DNA binding reactions
(Kellar, Oliver)
8. Biohazard sorting
(Schmid, Roederer, Koup, Ambrozak, Perfetto)
9. Guidelines for the presentation of flow cytometric data.
(Roederer, Darzynkiewicz, Paks)
Part II: Cellular DNA Content Analysis
10. VinDetergent and proteolytic enzyme-based techniques for nuclear isolation and DNA content analysis
(Vindelov,Christensen)
11. Rapid DNA content analysis
(Krishan)
12. DNA analysis from paraffin-embedded blocks
(Hedley)
13. Flow cytometry and sorting of plant protoplasts and cells
(Galbraith)
14. DNA content histogram and cell cycle analysis.
(Rabinovitch)
15. Simultaneous analysis of cellular RNA and DNA content
(Darzynkiewicz)
Part III. Cell Proliferation and Death Assays
16. Immunochemical quantitation of bromodeoxyuridine: application to cell cycle kinetics
(Dolbeare, Selden)