Mitochondrial DNA
Methods and Protocols
197th Edition
Published on 1. January 2002
Book
Hardback
XIV, 414 pages
978-0-89603-972-8 (ISBN)
Description
Internationally recognized authorities describe in great detail the methods they have perfected to analyze mtDNA and the proteins involved in its maintenance. The analytical techniques cover the purification of mtDNA from a variety of sources and the analysis of DNA for both deletions, point mutations, and damage, for replication intermediates, and for following the fate of mtDNA outside of the mitochondria. Additional analytical methods are presented for analyzing the proteins and enzymes that maintain mtDNA. Comprehensive and timely, "Mitochondrial DNA: Methods and Protocols" offers both basic and clinical researchers proven cutting-edge methods for analyzing mtDNA and illuminating the role it plays in the aging process, apoptosis, and possibly some cancers.
Reviews / Votes
Accordingly, the book is timely and of significant use to investigators working in this area. The book is divided into two major sections -- the first deals with analysis of the DNA and the second with analysis of related proteins. A valuable introductory chapter discusses available animal models for mitochondrial disease and the book concludes with a discussion of knockout mice. Given the general interest in mitochondrial mutations as they relate to a variety of human diseases, this contribution is timely and of value. The protocols are sufficiently detailed to allow adaptation into any working laboratory. - Doody's Health Sciences Book Review JournalMore details
Series
Edition
197th ed.
Language
English
Place of publication
NJ
United States
Target group
College/higher education
Professional and scholarly
Illustrations
120
8 farbige Abbildungen, 120 s/w Abbildungen
97 black & white illustrations, 6 colour illustrations, 23 black & white halftones, 74 black & white line drawings
Dimensions
Height: 22.9 cm
Width: 15.2 cm
Weight
1 gr
ISBN-13
978-0-89603-972-8 (9780896039728)
DOI
10.1385/1592592848
Schweitzer Classification
Other editions
New editions
Book
07/2009
2nd Edition
Humana Press Inc.
€106.99
Shipment within 15-20 days
Content
Part I. Methods for the Analysis of mtDNA
Animal Models for Mitochondrial Disease
Douglas C. Wallace
Identification of Mutations in mtDNA from Patients Suffering Mitochondrial Diseases
Eric A. Schon, Ali Naini, and Sara Shanske
Screening for Aging-Dependent Point Mutations in mtDNA
Yuichi Michikawa and Giuseppe Attardi
Scanning Low-Frequency Point Mutants in the Mitochondrial Genome Using Constant Denaturant Capillary Electrophoresis
Weiming Zheng, Luisa A. Marcelino, and William G. Thilly
Identification of mtDNA Mutations in Human Cancer
Shuji Nomoto, Montserrant Sanchez-Cespedes, and David Sidransky
In Situ PCR of the Common Human mtDNA Deletion: Is It Related to Apoptosis?
Steven J. Zullo
Reproducible Quantitative PCR of Mitochondrial and Nuclear DNA Copy Number Using the LightCyclerT
Alice Wong and Gino Cortopassi
Yeast Nuclear Genes for mtDNA Maintenance
Françoise Foury
Measuring mtDNA Mutation Rates in Saccharomyces cerevisiae Using the mtArg8 Assay
Micheline K. Strand and William C. Copeland
Measuring Oxidative mtDNA Damage and Repair Using Quantitative PCR
Janine H. Santos, Bhaskar S. Mandavilli, and Bennett Van Houten
Migration of mtDNA into the Nucleus
Mary K. Thorsness, Karen H. White, and Peter E. Thorsness
2D Gel Electrophoresis of mtDNA
Heather E. Lorimer
Part II. Methods for the Analysis of Proteins Involved in mtDNA Transactions
Purification of Mitochondria for Enzymes Involved in Nucleic Acid Transactions
Daniel F. Bogenhagen
Purification of Mitochondrial Uracil-DNA Glycosylase Using Ugi-Sepharose Affinity Chromatography
Samuel E. Bennett, Mary Jane Shroyer, Jung-Suk Sung, and Dale W. Mosbaugh
Characterization of Specialized mtDNA Glycosylases
Simon G. Nyaga and Vilhelm A.Bohr
Purification, Separation, and Identification of the Human mtDNA Polymerase With and Without Its Accessory Subunit
Matthew J. Longley and William C. Copeland
Assay of mtDNA Polymerase g from Human Tissues
Robert K. Naviaux
Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form: DNA Polymerase g
Carol L. Farr and Laurie S. Kaguni
Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form: Mitochondrial Single-Stranded DNA-Binding Protein
Carol L. Farr and Laurie S. Kaguni
Expression, Purification and In Vitro Assays of Mitochondrial Single-Stranded DNA-Binding Protein
Kang Li and R. Sanders Williams
Recombinant Yeast mtDNA Helicases: Purification and Functional Assays
Silja Kuusk, Tiina Sedman, and Juhan Sedman
Isolation of DNA Topoisomerases I and II Activities from Mitochondria of Mammalian Heart
Robert L. Low
Endonuclease G Isolation and Assays
Robert L. Low and Mariana Gerschenson
Targeting DNA Repair Proteins to Mitochondria
Allison W. Dobson, Mark R. Kelley, Glenn L. Wilson, and Susan P. LeDoux
In Organello Footprinting of mtDNA
Steven C. Ghivizzani, Cort S. Madsen, Chandramohan V. Ammini, and William W. Hauswirth
Crosslinking of Proteins to mtDNA
Brett A. Kaufman, Scott M. Newman, Philip S. Perlman, and Ronald A. Butow
Breeding and Genotyping of Tfam Conditional Knockout Mice
Mats Ekstrand and Nils-Göran Larsson
Animal Models for Mitochondrial Disease
Douglas C. Wallace
Identification of Mutations in mtDNA from Patients Suffering Mitochondrial Diseases
Eric A. Schon, Ali Naini, and Sara Shanske
Screening for Aging-Dependent Point Mutations in mtDNA
Yuichi Michikawa and Giuseppe Attardi
Scanning Low-Frequency Point Mutants in the Mitochondrial Genome Using Constant Denaturant Capillary Electrophoresis
Weiming Zheng, Luisa A. Marcelino, and William G. Thilly
Identification of mtDNA Mutations in Human Cancer
Shuji Nomoto, Montserrant Sanchez-Cespedes, and David Sidransky
In Situ PCR of the Common Human mtDNA Deletion: Is It Related to Apoptosis?
Steven J. Zullo
Reproducible Quantitative PCR of Mitochondrial and Nuclear DNA Copy Number Using the LightCyclerT
Alice Wong and Gino Cortopassi
Yeast Nuclear Genes for mtDNA Maintenance
Françoise Foury
Measuring mtDNA Mutation Rates in Saccharomyces cerevisiae Using the mtArg8 Assay
Micheline K. Strand and William C. Copeland
Measuring Oxidative mtDNA Damage and Repair Using Quantitative PCR
Janine H. Santos, Bhaskar S. Mandavilli, and Bennett Van Houten
Migration of mtDNA into the Nucleus
Mary K. Thorsness, Karen H. White, and Peter E. Thorsness
2D Gel Electrophoresis of mtDNA
Heather E. Lorimer
Part II. Methods for the Analysis of Proteins Involved in mtDNA Transactions
Purification of Mitochondria for Enzymes Involved in Nucleic Acid Transactions
Daniel F. Bogenhagen
Purification of Mitochondrial Uracil-DNA Glycosylase Using Ugi-Sepharose Affinity Chromatography
Samuel E. Bennett, Mary Jane Shroyer, Jung-Suk Sung, and Dale W. Mosbaugh
Characterization of Specialized mtDNA Glycosylases
Simon G. Nyaga and Vilhelm A.Bohr
Purification, Separation, and Identification of the Human mtDNA Polymerase With and Without Its Accessory Subunit
Matthew J. Longley and William C. Copeland
Assay of mtDNA Polymerase g from Human Tissues
Robert K. Naviaux
Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form: DNA Polymerase g
Carol L. Farr and Laurie S. Kaguni
Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form: Mitochondrial Single-Stranded DNA-Binding Protein
Carol L. Farr and Laurie S. Kaguni
Expression, Purification and In Vitro Assays of Mitochondrial Single-Stranded DNA-Binding Protein
Kang Li and R. Sanders Williams
Recombinant Yeast mtDNA Helicases: Purification and Functional Assays
Silja Kuusk, Tiina Sedman, and Juhan Sedman
Isolation of DNA Topoisomerases I and II Activities from Mitochondria of Mammalian Heart
Robert L. Low
Endonuclease G Isolation and Assays
Robert L. Low and Mariana Gerschenson
Targeting DNA Repair Proteins to Mitochondria
Allison W. Dobson, Mark R. Kelley, Glenn L. Wilson, and Susan P. LeDoux
In Organello Footprinting of mtDNA
Steven C. Ghivizzani, Cort S. Madsen, Chandramohan V. Ammini, and William W. Hauswirth
Crosslinking of Proteins to mtDNA
Brett A. Kaufman, Scott M. Newman, Philip S. Perlman, and Ronald A. Butow
Breeding and Genotyping of Tfam Conditional Knockout Mice
Mats Ekstrand and Nils-Göran Larsson