Amino Acid Analysis Protocols
Published on 10. November 2010
Book
Paperback/Softback
XI, 265 pages
978-1-61737-117-2 (ISBN)
Description
Amino acid analysis is a technique that has become commonplace in biotechnology, biomedical, and food analysis laboratories. This book describes a variety of amino acid analysis techniques and how each technique can be used to answer specific biological questions. The first two chapters in Amino Acid Analysis Protocols introduce the concepts, basic theory, and practice of amino acid analysis. The following chapters give detailed instructions on various methods and their applications. As highlighted, there are many different approaches to amino acid ana- sis, but in all cases the results depend heavily on the quality of the sample. Therefore a new way to desalt samples prior to hydrolysis is covered as an introductory chapter (Chapter 3), and most authors have devoted a section to sample preparation, especially to the collection and storage of bodily fluids. Some of the amino acid analysis methods described in this book are based on HPLC separation and analysis after precolumn derivatization.
The precolumn derivatization techniques described use (a) 6-aminoquinolyl-N-hydro- succinimidyl carbamate (AQC) (Chapters 4 and 8); (b) 1-fluoro-2- dinitrophenyl-5-L-alanine amide (Marfey's reagent), which allows sepa- tion and analysis of enantiomeric amino acids (Chapter 5); (c) O-phthalal- hyde (OPA) (Chapters 6 and 10); (d) butylisothiocyanate (BITC) and benzylisothiocyante (BZITC) (Chapter 11); (e) phenylisothiocyanate (PITC) (Chapters 12 and 13); (f) ammonium-7-fluorobenzo-2-oxa-1,3-diazole-4-s- fonate (SBD-F) (Chapter 17); and (g) 9-fluorenylmethyl-chloroformate (FMOC-Cl) (Chapter 10).
The precolumn derivatization techniques described use (a) 6-aminoquinolyl-N-hydro- succinimidyl carbamate (AQC) (Chapters 4 and 8); (b) 1-fluoro-2- dinitrophenyl-5-L-alanine amide (Marfey's reagent), which allows sepa- tion and analysis of enantiomeric amino acids (Chapter 5); (c) O-phthalal- hyde (OPA) (Chapters 6 and 10); (d) butylisothiocyanate (BITC) and benzylisothiocyante (BZITC) (Chapter 11); (e) phenylisothiocyanate (PITC) (Chapters 12 and 13); (f) ammonium-7-fluorobenzo-2-oxa-1,3-diazole-4-s- fonate (SBD-F) (Chapter 17); and (g) 9-fluorenylmethyl-chloroformate (FMOC-Cl) (Chapter 10).
Reviews / Votes
"Sufficient detail and reference material is provided to allow application by a chemist generally familiar with analytical methods. Choices among the various procedures described will likely be made on an empirical basis."-Doody's Health Sciences Book Review Journal"...the book presents a timely and detailed methodological reference for a broad range of current techniques used in amino acid analysis. The authors are all experts in the field and the editors (especially Williams) have considerable experience in amino acid analysis. this volume will provide an indispensable tool in any laboratory carrying out amino acid analysis."- Today's Life Science
"...This is a major collection of analytical techniques devoted to the analysis of amino acids in biotechnology, food analysis, and biomedical research, as well as the amino acid content of patient samples in clinical laboratories...This book is highly recommended for both engineers and experienced analysts interested in amino acid analysis; both will benefit from the information contained in this collection. It might also be a helpful resource for those looking for a replacement for old, dedicated amino acid analyzers for physiological samples by methods based on newer technologies and alternative techniques." - Clinical Chemistry
"Up-to-date and highly practical, Amino Acid Analysis Protocols offers analytical and clinical chemists as well as a broad range of biological and biomedical investigators a richer compendium of laboratory tools for the productive analysis of both common and uncommon amino acids." - Journal of Chromatographic Science
More details
Series
Edition
1st ed. Softcover of orig. ed. 2000
Language
English
Place of publication
Totowa
United States
Target group
Professional and scholarly
Professional/practitioner
Product notice
Paperback (trade)
Unsewn / adhesive bound
Illustrations
XI, 265 p.
Dimensions
Height: 229 mm
Width: 152 mm
Thickness: 16 mm
Weight
413 gr
ISBN-13
978-1-61737-117-2 (9781617371172)
DOI
10.1385/1592590470
Schweitzer Classification
Other editions
Additional editions
Catherine Cooper
Amino Acid Analysis Protocols
Book
09/2000
Humana Press Inc.
€106.99
Shipment within 15-20 days
Content
Amino Acid Analysis.- Amino Acid Analysis, Using Postcolumn Ninhydrin Detection, in a Biotechnology Laboratory.- Purification of Proteins Using UltraMacro Spin Columns or ProSorb Sample Preparation Cartridges for Amino Acid Analysis.- Amino Acid Analysis Using Precolumn Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate.- Amino Acid Analysis by High-Performance Liquid Chromatography after Derivatization with 1-Fluoro-2,4-Dinitrophenyl-5-L-Alanine Amide (Marfey's Reagent).- The Analysis of Amino Acids Using Precolumn Derivatization, HPLC, and Electrochemical Detection.- Anion Exchange Chromatography and Integrated Amperometric Detection of Amino Acids.- Ion-Pair Chromatography for Identification of Picomolar-Order Protein on a PVDF Membrane.- Capillary Gas Chromatographic Analysis of Protein and Nonprotein Amino Acids in Biological Samples.- Measurement of Blood Plasma Amino Acids in Ultrafiltrates by High-Performance Liquid Chromatography with Automatic Precolumn O-Phthaldialdehyde Derivatization.- Determination of Amino Acids in Foods by Reversed-Phase High-Performance Liquid Chromatography with New Precolumn Derivatives, Butylthiocarbamyl, and Benzylthiocarbamyl Derivatives Compared to the Phenylthiocarbamyl Derivative and Ion Exchange Chromatography.- Amino Acid Measurement in Body Fluids Using PITC Derivatives.- Determination of Proteins, Phosphatidylethanolamine, and Phosphatidylserine in Lipid-Rich Materials by Analysis of Phenylthiocarbamyl Derivatives.- Analysis of O-Phosphoamino Acids in Biological Samples by Gas Chromatography with Flame Photometric Detection.- Determination of Sulfur Amino Acids, Glutathione, and Related Aminothiols in Biological Samples by Gas Chromatography with Flame Photometric Detection.- Capillary Electrophoretic Determination of 4-Hydroxyproline.- Total Plasma Homocysteine Analysis by HPLC with SBD-F Precolumn Derivatization.- Determination of Early Glycation Products by Mass Spectrometry and Quantification of Glycation Mediated Protein Crosslinks by the Incorporation of [14C]lysine into Proteins.