Bacterial Pathogenesis

Working with Pathogenic Bacteria and Evaluations of Virulence:
S.A. Morse and J.E. McDade, Recommendations for Working with Pathogenic Bacteria.
S. Welkos and A. O'Brien, Determination of Median Lethal and Infectious Doses in Animal Model Systems.
Species and Strain Identification:
C.E. Frasch, Serogroup and Serotype Classification of Bacterial Pathogens.
K. Nelson and R.K. Selander, Analysis of Genetic Variation by Polymerase Chain Reaction-Based Nucleotide Sequencing.
R. Rudner, B. Studamire, and E.D. Jarvis, Determination of Restriction Fragment Length Polymorphism in Bacteria Using Ribosomal RNA Genes.
D. van Soolingen, P.E.W. De Haas, P.W.M. Hermans, and J.D.A. van Embden, DNA Fingerprinting of Mycobacterium tuberculosis. T.M. Schmidt and D.A. Relman, Phylogenetic Identification of Uncultured Pathogens Using Ribosomal RNA Sequences.
Bacterial Cell Fractionation:
H. Nikaido, Isolation of Outer Membranes.
M.A. Apicella, J.M. Griffiss, and H. Schneider, Isolation and Characterization of Lipopolysaccharides, Lipooligosaccharides, and Lipid A.
R.S. Rosenthal and R. Dziarski, Isolation of Peptidoglycan and Soluble Peptidoglycan Fragments.
V.A. Fischetti, Purification of Streptococcal M Protein.
W.F. Vann and S.J. Freese, Purification of Escherichia coli K Antigens.
G.K. Schoolnik, Purification of Somatic Pili.
Microbial Acquisition of Iron:
S.M. Payne, Detection, Isolation, and Characterization of Siderophores.
C.N. Cornelissen and P.F. Sparling, Identification of Receptor-Mediated Transferrin-Iron Uptake Mechanism in Neisseria gonorrhoeae.
Genetics and Regulation:
V. De Lorenzo and K.N. Timmis, Analysis and Construction of Stable Phenotypes in Gram-Negative Bacteria with Tn5 and Tn10-Derived Minitransposons.
R.A. Burne and R.G. Quivey, Jr., Use of Transposons to Dissect Pathogenic Strategies of Gram-Positive Bacteria.
M.R. Kaufman and R.K. Taylor, Identification of Bacterial Cell-Surface Virulence Determinants with TnphoA. A.M. Hooke, Temperature-Sensitive Mutants of Bacterial Pathogens: Isolation and Use to Determine Host Clearance and in Vivo Replication Rates.
S. Stibitz, Use of Conditionally Counterselectable Suicide Vectors for Allelic Exchange.
J.M. Slauch, M.J. Mahan, and J.J. Mekalanos, In Vivo Expression Technology for Selection of Bacterial Genes Specifically Induced in Host Tissues.
Enzyme and Toxin Assays:
M.S. Lantz and P. Ciborowski, Zymographic Techniques for Detection and Characterization of Microbial Proteases.
L. Passador and W. Iglewski, ADP-Ribosylating Toxins.
G.E. Rowe and R.A. Welch, Assays of Hemolytic Toxins.
M. Thelestam and I. Florin, Assay of Cytopathogenic Toxins in Cultured Cells.
B.L. Kagan and Y. Sokolov, Use of Lipid Bilayer Membranes to Detect Pore Formation by Toxins.
C.B. Saelinger and R.E. Morris, Uptake and Processing of Toxins by Mammalian Cells.
Bacterial Effects on Immune Cells:
H. Loppnow, H. Brade, E.T. Rietchel, and H-D. Flad, Induction of Cytokines in Mononuclear and Vascular Cells by Endotoxin and Other Bacterial Products.
M.S. Detrick, R. Kreisberg, and R.N. Moore, Effect of Bacterial Products on Colony-Stimulating Factor Production.
Z.A. McGee and C.M. Clemens, Effect of Bacterial Products on Tumor Necrosis Factor Production: Quantification of TNF in Biologic Fluids or Tissues.
K-I. Tanamoto, Induction of Prostaglandin Release from Macrophages by Bacterial Endotoxin.
G. Sonnenfeld, Effect of Bacterial Products on Interferon Production.
Phagocytosis and Killing Assays:
R.F. Rest and D.P. Speert, Measurement of Nonopsonic Phagocytic Killing by Human and Mouse Phagocytes.
J.R. Kalmar, Measurement of Opsonic Phagocytosis by Human Polymorphonuclear Neutrophils.
R.F. Rest, Measurement of Human Neutrophil Respiratory Burst Activity during Phagocytosis of Bacteria.
D.P. McQuillen, S. Gulati, and P.A. Rice, Complement-Mediated Bacterial Killing Assays.
T.H. Steinberg and J.A. Swanson, Measurement of Phagosome-Lysosome Fusion and Phagosomal pH.
S.S.S.L. Harwig, T. Ganz, and R.I. Lehrer, Neutrophil Defensins: Purification, Characterization, and Antimicrobial Testing.
Adherence:
J. Goldhar, Bacterial Lectinlike Adhesins: Determination and Specificity.
R.E. Mandrell, M.A. Apicella, R. Lindstedt, and H. Leffler, Possible Interaction between Animal Lectins and Bacterial Carbohydrates.
G.T. Van Nhieu and R.R. Isberg, Isolation and Identification of Eukaryotic Receptors Promoting Bacterial Internalization.
E. Rozdzinski and E. Tuomanen, Interactions of Bacteria with Leukocyte Integrins.
C.D. Deal and H.C. Krivan, Solid-Phase Binding of Microorganisms to Glycolipids and Phospholipids.
P. Falk, T. Boren, and S. Normark, Characterization of Microbial Host Receptors.
Invasion and Intracellular Survival in Eukaryotic Cells:
E.A. Elsinghorst, Measurement of Invasion by Gentamicin Resistance.
J.P.M. van Putten, J.F.L Weel, and H.U.C. Grassme, Measurements of Invasion by Antibody Labeling and Electron Microscopy.
M.G. Pucciarelli and B.B. Finley, Polarized Epithelial Monolayers: Model Systems to Study Bacterial Interactions with Host Epithelial Cells.
D.A. Haake and M.A. Lovett, Interjunctional Invasion of Endothelial Cell Monolayers.
S. Jones and D.A. Portnoy, Intracellular Growth of Bacteria.
I. Rosenshine, S. Ruschkowski, and B.B. Finlay, Inhibitors of Cytoskeletal Function and Signal Transduction to Study Bacterial Invasion.
L.G. Tilney and M.S. Tilney, Methods to Visualize Actin Polymerization Associated with Bacterial Invasion.
J.L. Pace and J.E. Galan, Measurement of Free Intracellular Calcium Levels in Epithelial Cells as Consequence of Bacterial Invasion.
Identification of Genes Involved in Invasion:
R. Menard and P.J. Sansonetti, Shigella flexneri: Isolation of Noninvasive Mutants of Gram-Negative Pathogens.
F. Bowe and F. Heffron, Isolation of Salmonella Mutants Defective for Intracellular Survival.
S. Jones and D.A. Portnoy, Small Plaque Mutants.
Subject Index.