Enzyme Engineering

Conference History.- The First Ten Years.- Keynote Paper.- Recent Studies on Antibiotics and Low Molecular Weight Enzyme Inhibitors.- Session I. Large Scale Production and Purification of Biomolecules.- Integration of Large Scale Production and Purification of Biomolecules.- Role of Active Transport of Inducer in Enzyme Biosynthesis and Process Scale-Up.- Multi Enzyme Systems in Membrane Reactors.- Scale-Up of Protein Purification by Liquid-Liquid Extraction.- Rapid and Large-Scale Purification of Angiotensin-I Forming Enzymes and Milk Clotting Enzymes by Affinity Chromatography.- Separation and Purification of Enzymes Via Continuous Parametric Pumping.- Session II. Applications of Biocatalysts for New Reactions and Organic Synthesis.- Enzymatic Synthesis of Penicillins and Cephalosporins by Penicillin Acylase.- Enzymatic Acyl Transfer in Penicillin and Cephalosporin Chemistry.- Enzymatic Processes for the Synthesis of Optically Active Amino Acids.- Horse Liver Alcohol Dehydrogenase: An Illustrative Example of the Potential of Enzymes in Organic Synthesis.- Immobilized Enzymes and Synzymes: Applications in Organic Synthesis.- Immobilization of Living Microbial Cells and Transformation of Steroids.- Steroid Bioconversion by Immobilized Cells.- Steroid ?1-Dehydrogenase Isoenzymes in Corynebacterium Species Choi 73 T 191.- Use of Immobilized Enzyme Requiring Cofactor Regeneration and of Immobilized Mycelium, for Steroid Modification.- Hydroxylation of Steroids by Immobilized Microbial Cells.- Enzymatic Synthesis of Sulfur and Selenium Amino Acids.- Production of Alkaloids with Immobilized Cells of Catharanthus Roseus.- Propyleneoxide Production by Immobilized Nocardia Corallina B-276.- Air-Oxidation of Linoleic Acid by Lipoxygenase-Containing Particles Suspended in Water-insoluble Organic Solvent.- Progress Toward Artificial Photosynthesis.- Light-Sensitization of a Microbial Protease.- Enzymatic Photosensitive Materials.- Ester Exchange of Triglyceride by Entrapped Lipase in Organic Solvent.- Solvent Production by Clostridium Acetobutylicum in Aqueous Two-Phase Systems.- Enzyme Kinetics and Mass-Transfer on Two Liquid Phase Heterogeneous Systems.- Physico-chemical Means of Increasing the Yield of End Products in Biocatalysis.- Heat-Stable L-Lactate Dehydrogenase and its Application to an Enzyme Reactor.- Multienzymes and Cofactors Immobilized within Lipid-Polyamide Membrane Microcapsules for Sequential Substrate Conversion.- Tyrosine and 3,4-Dihydroxyphenyl-Alanine Synthesis by Citrobacter Freundii.- Enzyme Catalysts for the Stereo and Regio Selective Oxyfunctionalization of Organic Substrates.- Session III. New Immobilization Techniques of Biomaterials and their Applications.- High-Rate, Continuous Waste Processor for the Production of High BTU Gas Using Immobilized Microbes.- New Developments in the Preparation and Characterization of Polymerbound Biocatalysts.- Bioconversion of Lipophilic or Water Insoluble Compounds by Immobilized Biocatalysts in Organic Solvent Systems.- Multiple Carrier Regeneration and Enzyme Immobilization In Situ.- Viability and Biosynthetic Capacity of Immobilized Plant Cells.- Immobilized Enzymes of Heterogenous Structure.- Fibrous Support for Immobilization of Enzymes.- High Temperature Cell-Trapped Ultrafiltration Membranes.- Immobilized Modifiers for Proteins (IMPs).- Enzyme Immobilization in Porous Supports.- Immobilization of Microbial Cells Using Gelatin and Glutaraldehyde.- Immobilization of ?-Galactosidase from E. Coli CSH36 and Its Microbial Cells Using Cellulose Beads.- Hydrophobic Immobilization of Enzymes and Polynucleotides on Trityl Agarose.- Influence of the Activation Degree of the Support on the Properties of Agarose-Nuclease.- Immobilization of Biofunctional Components by Radiation Polymerization and Applications.- Immobilization of Whole Cell Glucose Isomerase within Soybean Protein.- Enzyme Stabilization in High Macromolecular Concentration Environments.- Intrinsic Stability of Thermophilic Enzymes: 6-Phosphogluconate Dehydrogenase from Bacillus Stearothermophilus and Yeast.- Stabilization of Penicillin Amidohydrolase Immobilized on Eupergit C.- Stabilization of Fumarase Activity of Brevibacterium Flavum Cells by Immobilization with k Carrageenan and Polyethyleneimine.- Application of Polyethylene Glycol-Bound NAD Derivative and Thermostable Dehydrogenase in a Model Enzyme Reactor.- Use of the Porous Mineral Spherosil as a Carrier for Enzymes: Fixation and Purification.- Session IV. Industrial Applications of Immobilized Biomaterials.- Potential Application of Immobilized Viable Cells in the Food Industry: Malolactic Fermentation of Wine.- Production of L-Tryptophan with Immobilized Cells.- Applications of Immobilized Tannin for Protein and Metal Adsorption.- Industrial Applications of Immobilized Biomaterials in China.- Production of L-Alanine from Ammonium Fumarate Using Two Types of Immobilized Microbial Cells.- New Process for Production of High Fructose Corn Syrup Using Combined Adsorption and an Enzyme Reactor.- Immobilized Multienzyme Systems for Starch Processing.- Glucoamylase Covalently Coupled to Porous Glass.- Rotary Multidisc Reactor of Collagen Supported Immobilized Glucoamylase.- Kinetics for the Hydrolysis of Soluble Starch by Glucoamylase and Application to an Immobilized Enzyme System.- Reduced Thermostability of Modified Mucor Miehei Rennet.- Application of Immobilized Enzymes to Milk Curdling..- Production of 7-Aminodesacetoxycephalosporanic Acid by Immobilized E. coli Cells.- Technical Applications of Lactase and Aminoacid Acylase Iiranobilized to Form Plexazym.- Continuous Hydrolysis of Lactose in Skim Milk and Acid Whey by Immobilized Lactase of Aspergillus Oryzae.- Lactic Acid Fermentation with Immobilized Lactobacillus Sp.- Production of 1-Malic Acid with Immobilized Thermophilic Bacteriumr Thermus Rubens Nov. sp.- Continuous Hydrolysis of Concentrated Sucrose Solutions by Immobilized Invertase.- A Series of Covalently Bonded Enzymes and Their Applications.- The Application of Fluidized Beds for Improved Enzyme Reactor Performance.- Control of Continuous Coenzyme Regeneration.- A New Approach to Membrane Reactor Design and Operation.- Influence of Compaction in Gel-Immobilized Enzyme Packed Bed Reactors.- Session V. Biomass Conversion with Energy Production.- Enzymatic Removal of Hazardous Pollutants from Industrial Aqueous Effluents.- Enzymatic Hydrolysis of Cellulose: Effects of Structural Properties of Cellulose on Hydrolysis Kinetics.- Continuous Ethanol Fermentation by Immobilized Biocatalysts.- An Immobilized Yeast Cell Column for the Fermentation of Molasses.- Pilot Operation for Continuous Alcohol Fermentation of Molasses in an Immobilized Bioreactor.- Enzymatic Hydrolysis of Cellulose.- ATP Regeneration by Enzymes of Alcohol Fermentation and Kinases of Yeast and Its Computer Simulation.- Extracellular Cellulases Produced by a Yeast-Like Fungus.- Relationship Between Extracellular Proteases and the Cellulase Complex of Trichoderma Reesei.- Session VI. Analytical Applications of Immobilized Biomaterials.- Binding Assays Involving Separation in Aqueous Two-Phase Systems: Partition Affinity Ligand Assay (PALA).- Use of Immobilized Enzyme Reactors in Automated Clinical Analyses.- Immobilized Enzymes in Analysis: Applications and Economic Aspects.- Microbial Sensors for Gas Analysis.- Analytical Uses of Immobilized Enzymes.- Cost Analysis and Viability of Immobilized Enzymes in Routine Analysis.- Multipurpose Enzyme-Collagen Membrane Electrodes.- Long-Term Stability of Air-Dried Enzyme Electrodes with Selective Enzymic Collagen Membranes.- Enzyme Electrodes Based on Insolubilized Enzyme Membranes Coupled with an Electrochemical Transducer.- Potentiometrie Glucose Sensor: Enzymatic Activity and Potentiometrie Measurements.- Microbial Sensor for Preliminary Screening of Mutagens.- Application of Microbial Electrode to Analysis of Waste Water.- Biosensors Based on Enzyme Amplification and Immunochemical Selectivity.- Application of Chemiluminescence of Cypridina Luciferin Analog to Immobilized Enzyme Sensors.- Use of Hydrogen Sensitive Pd-MOS Components in Biochemical Analysis.- Computer Controlled Mass Spectrometer Monitoring of Fermentations.- Session VII. Medical Applications of Enzyme Technology.- Immobilized Heparinase: Production, Purification, and Application in Extracorporeal Therapy.- Clinical Utility of Urokinase-Treated Polymer for Antithrombogenic Material.- Artificial Cell Immobilized Multienzyme Systems and Cofactors.- Antithrombogenic Activity of Artificial Medical Materials Improved by Enzyme Immobilization Techniques.- Application of Immobilized Enzymes for Biomaterials Used in the Field of Thoracic Surgery.- Use of Immobilization Principles for the Construction of Drug Targeting Systems.- Application of Bioreactors with Immobilized L-Asparaginase.- Acyl-CoA Synthetase and Acyl-CoA Oxidase for Determination of Serum Free Fatty Acids.- Enzyme Immunoassay for Free Thyroxine.- Routine Determination of Hydrogen Peroxide in Clinical Chemistry with Immobilized Aldehyde Dehydrogenase.- Enzyme Electrodes for Simultaneous Determination of Creatinine and Creatine in Serum or Whole Blood.- Session VIII. Genetic Engineering for Enzyme (Or Important Biological Substances) Production.- Construction of Various Host Vector Systems and the Variation of Enzyme Levels.- Enzymes Active on Unnatural Synthetic Compounds: Nylon Oligomer Hydrolases Controlled by a Plasmid and Their Cloning.- Closing Session.- Recent Developments and Future Aspects of Enzyme Engineering.- List of Participants.