Chambers Biochemistry Labfax

?Contributors
Abbreviations
1. Buffers, Chelating Agents And Denaturants
Buffers
Introduction
Goods' Buffers
Preparation of Buffers
Counter Ions
Effects of Temperature and Concentration
Biological Compatibility and Chemical Reactivity
Preparation of Phosphate Buffers
Preparation of Acetate Buffers
Buffered Salines, Balanced Salts and Osmotic Supports
Chelating Agents
Denaturing Agents for Proteins
Mechanisms of Denaturing Agents
Mixing Denaturants
Characteristics of Denaturants and Precipitants
Thiol Reagents
Detergents
Properties of Acids, Bases, Salts and Organic Solvents
Amino Acids
Ammonium Sulfate Precipitation Chart for Proteins
References
2. Radioisotopes in Biochemistry
Definitions
Units of Radioactivity
Specific Activity
Electron Volt (eV, or MeV=106 eV)
Gray
Dose Equivalent Man (Sievert)
Radioisotopes Used in Biochemistry
Radioactive Decay Correction
Autoradiography
Scintillation Counting
Cerenkov Counting
Counting of Finely Dispersed or Solvent-Soluble Substances
Counting of Aqueous Samples
Gamma Counting
Use of Radioisotopes as Tracers
Radiological Protection
Shielding for �-Particles and ?-Rays
Methods for Decontaminating Laboratories
References
Further Reading
3. Chromatographic Fractional Media
Introduction
Media for Size Exclusion
Media for Ion-Exchange Separations
Media for Reversed-Phase Separations
Media for Affinity Chromatography
Manufacturers and Suppliers of Chromatography Column Support Materials
References
Further Reading
4. Electrophoresis of Proteins and Nucleic Acids
Proteins
Separations on Denaturing Gels
Separations on Non-Denaturing Gels
Separations of Proteins by Isoelectric Focusing
Two-Dimensional Gel Electrophoresis
Marker Proteins
Staining Protein Gels
Nucleic Acids
Gels for Separating Nucleic Acids and Nucleoproteins
Markers for Nucleic Acids
Staining Nucleic Acid Gels
Methods Manuals on Electrophoresis
5. General Centrifugation Data
Calculation of Centrifugal Force
Applications of Centrifuge Rotors
Calculation of k-Factors of Rotors and Pelleting Times
Derating Rotors for Use with Dense Solutions
Properties of Centrifuge Tubes and Bottles
Sterilization and Disinfection Procedures
Sterilization Techniques
Biological Disinfection
Equations Relating the Refractive Index to the Density of Solutions
Properties of Sucrose Solutions
6. Enzymology
Introduction
Enzyme Kinetics
Enzyme Kinetics of Single Substrate Reactions
Sigmoidal Kinetics and Allosteric Enzymes
Enzyme Kinetics of Bisubstrate Reactions
Enzyme Inhibitors
Reversible Inhibition
Irreversible Inhibition
Effects of pH on Enzymes
Coenzymes - Structure and Functions
Enzyme Assays
Handling and Storage of Enzymes and Coenzymes
References
Further Reading
7. Hydrolytic Enzymes
Introduction
Inhibition of Nucleases and Proteases
Deoxyribonucleases
Ribonucleases
Proteases
Carbohydrases
Naturally Occurring or Physiological Inhibitors
References
8. Characteristics of Selected Proteins
References
9. Glycoproteins and Protein Glycosyution
Introduction
Occurrence of Glycosylated Proteins in Nature
Range and Types of Glycosylated Proteins
Structural Features of Glycosylated Proteins
Linkage of Carbohydrate to Proteins
Oligosaccharide Structures
Enzymic Glycosylation - Synthesis of Sequence
Monosaccharide Transport, Interconversion and Activation
Glycosyltransferases
?xamples of Functional Roles for Protein Glycosylation
References
10. Chemical and Post-Translational Modification of Proteins
Chemical Modification of Proteins
Enzyme-Catalyzed Covalent Modification Reactions
Introduction
Influence of Modification
Characteristics of Modification Reactions
Physiological Role of the Modification
Reversibility of Reactions
Summary
References
11. Nucleic Acids and their Components
Nucleosides and Nucleotides
Basic Structures
Nucleotides as Acids
Optical Density of Bases, Nucleosides and Nucleotides
Nucleotide-Derived Compounds
Selected Nucleotide Analogs
DNA of Selected Organisms
The Genetic Code
Abbreviations of Amino Acids
Assays for Nucleic Acids
References
12. Lipids
Introduction
Structure and Characteristics of Acyl Lipids
Fatty Acids
Neutral Acyl Lipids - Wax Esters, Acylglycerols and Glycerol Ethers
Glycerophospholipids
Glyceroglycolipids
Sphingolipids
Structure and Distribution of Terpenoid Constituents of Membranes
Sterols
Chlorophylls and Carotenoids
Composition and Distribution of Lipids in Membranes
Composition of Membrane Lipids
Fatty Acid Composition of Membrane Lipids
Structure and Properties of Bioactive Lipids
Eicosanoids
Platelet Activating Factor
Diacylglycerol
Steroid Hormones
Structure and Composition of Bile Acids and Bile Salts
Chemical Abstracts Registry Numbers of Lipids Cited
References
13. Carbohydrates and Sugars
Introduction
Structures and Characteristics of Monomeric Carbohydrates
Classification of Monosaccharides
Distribution and Properties of some Monosaccharides
Stereoisomerism
Oligosaccharides
Classification
Distribution and Properties
Structures and Characteristics of Polysaccharides
Classification
Plant Polysaccharides
Bacterial Polysaccharides
Animal Polysaccharides
DNA, RNA, Nucleosides and Nucleotides
Other Saccharide Derivatives
Further Reading
14. Safety and the Disposal of Toxic and Infectious Materials
Protection from Chemical Hazards
Risk and Safety Classification Systems
Disposal of Toxic and Infectious Materials
Disposal of Toxic Materials
Disposal of Biohazardous Materials
References
15. Sources of Further Biochemical Data
Reference Books
Databases
Nucleic Acid Sequence Databases
Contact Addresses
16. Atomic Weights and Mathematical Formulae
Atomic Weights
Mathematical Formulae
Lengths, Areas and Volumes in some Common Geometric Figures
Inter-Relations of Sides and Angles in a Plane Triangle
Trigonometrical Data
Mathematical Series
Mathematical Constants
Standard Equations
Differentials and Integrals
Index