Molecular Biology LabFax: Volume 2
Gene Analysis
2nd Edition
Published on 6. July 1998
Book
Hardback
255 pages
978-0-12-136110-5 (ISBN)
Description
In the eight years since the original publication of Molecular Biology Labfax, there has been a vast proliferation of molecular biology techniques. The Second Edition has been divided into two parts: Recombinant DNA, and Gene Analysis. Together they comprise a comprehensive collection of the most up-to-date methods available in molecular biology. This second volume of the two-part Second Edition provides key information on nucleic acid blotting and hybridization, DNA sequencing, PCR, labelingnucleic acids, electrophoresis, centrifugation, chemicals and reagents, and safety. Molecular Biology Labfax will be essential for scientists of all disciplines within the life sciences who use molecular biological techniques.
Reviews / Votes
"The book is compiled with care and contains text that explains technical terms that then can be used with understanding...especially useful for young investigators who are new to the field...this book qualifies to be the present-day standard reference..."-Amit Gupta, University of Illinois, in ASM NEWS (1999)More details
Series
Edition
2nd edition
Language
English
Place of publication
San Diego
United States
Publishing group
Elsevier Science Publishing Co Inc
Target group
Professional and scholarly
AUDIENCE: Research scientists at the postgraduate, postdoctoral level and above working in molecular biology, recombinant DNA, gene cloning, as well as other areas within the life sciences.
Edition type
New edition
Dimensions
Height: 248 mm
Width: 171 mm
Weight
720 gr
ISBN-13
978-0-12-136110-5 (9780121361105)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Person
Content
Abbreviations.
Nucleic Acid Blotting and Hybridization:
Membranes for Nucleic Acid Blotting.
Factors Influencing Hybridization.
Synthetic Oligonucleotide Probes.
Reagents and Conditions for Nucleic Acid Hybridization.
DNA Sequencing:
Chain Termination Sequencing.
Chemical Degradation Sequencing.
Denaturing Polyacrylamide Gel Electrophoresis.
Troubleshooting.
DNA Databases and Software for Sequence Analysis.
Polymerase Chain Reaction:
Amplification Methodology.
Cloning PCR Products.
Sequencing PCR Products.
Troubleshooting.
Labeling Nucleic Acids:
Radiochemistry.
Labeling Procedures.
Detection of Radioactivity.
Protection from Radiochemical Hazards.
Non-Radioactive Labeling.
Agarose Gel Electrophoresis:
Agarose Gels.
Electrophoresis Systems.
DNA and RNA Markers for Agarose Gels.
Centrifugation:
Calculation of the Relative Centrifugal Force.
Density Gradient Centrifugation.
Care of Rotors.
Chemicals and Reagents:
Details of Individual Compounds.
Safety:
Protection from Chemical Hazards.
Protection from Microbiological Hazards.
Nucleic Acid Blotting and Hybridization:
Membranes for Nucleic Acid Blotting.
Factors Influencing Hybridization.
Synthetic Oligonucleotide Probes.
Reagents and Conditions for Nucleic Acid Hybridization.
DNA Sequencing:
Chain Termination Sequencing.
Chemical Degradation Sequencing.
Denaturing Polyacrylamide Gel Electrophoresis.
Troubleshooting.
DNA Databases and Software for Sequence Analysis.
Polymerase Chain Reaction:
Amplification Methodology.
Cloning PCR Products.
Sequencing PCR Products.
Troubleshooting.
Labeling Nucleic Acids:
Radiochemistry.
Labeling Procedures.
Detection of Radioactivity.
Protection from Radiochemical Hazards.
Non-Radioactive Labeling.
Agarose Gel Electrophoresis:
Agarose Gels.
Electrophoresis Systems.
DNA and RNA Markers for Agarose Gels.
Centrifugation:
Calculation of the Relative Centrifugal Force.
Density Gradient Centrifugation.
Care of Rotors.
Chemicals and Reagents:
Details of Individual Compounds.
Safety:
Protection from Chemical Hazards.
Protection from Microbiological Hazards.