Microbiological Applications: Short Version

Laboratory Protocol Safety 1 Microscopy 1 Brightfield Microscopy 2 Darkfield Microscopy 3 Phase-Contrast Microscopy 4 Microscopic Measurements 2 Survey of Microorganisms 5 Protozoans, Algae, and Cyanobacteria 6 Ubiquity of Bacteria 7 The Fungi: Yeasts and Molds 3 Manipulation of Microorganisms 8 Aseptic Technique 9 Pure Culture Techniques 4 Staining and Observation of Microorganisms 10 Smear Preparation 11 Simple Staining 12 Negative Staining 13 Capsular Staining 14 Gram Staining 15 Spore Staining: Two Methods 16 Acid-Fast Staining: Ziehl-Neelsen Method 17 Motility Determination 5 Culture Methods 18 Culture Media Preparation 19 Cultivation of Anaerobes 20 Enumeration of Bacteria: The Standard Plate Count 21 Slime Mold Culture 22 Slide Culture: Molds 6 Bacterial Viruses 23 Determination of a Bacteriophage Titer 24 Isolation of Phage from Flies 25 Phage Typing 7 Environmental Influences and Control of Microbial Growth 26 Temperature: Effects on Growth 27 Temperature: Lethal Effects 28 pH and Microbial Growth 29 Water Activity and Osmotic Pressure 30 Ultraviolet Light: Lethal Effects 31 Oligodynamic Action 32 Evaluation of Alcohol: Its Effectiveness as an Antiseptic 33 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method 34 Evaluation of Antiseptics: The Filter Paper Disk Method 35 Effectiveness of Hand Scrubbing 8 Identification of Unknown Bacteria 36 Morphological Study of Unknown Bacterium 37 Cultural Characteristics 38 Physiological Characteristics: Oxidation and Fermentation Tests 39 Physiological Characteristics: Hydrolytic Reactions 40 Physiological Characteristics: Biochemical Tests 41 Use of Bergey's Manual 9 Miniaturized Multitest Systems 42 Enterobacteriaceae Identification: The API 20E System 43 Enterobacteriaceae Identification: The Enterotube II System 44 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System 45 Staphylococcus Identification: The API Staph-Ident System 10 Applied Microbiology 46 Bacterial Counts of Foods 47 Bacterial Examination of Water: Qualitative Tests 48 The Membrane Filter Method 49 Reductase Test 50 Microbial Spoilage of Canned Food 51 Microbiology of Alcohol Fermentation 11 Medical Microbiology and Immunology 52 The Staphylococci: Isolation and Identification 53 The Streptococci: Isolation and Identification 54 Gram Negative Intestinal Pathogens 55 Slide Agglutination Test: Serological Typing 56 Slide Agglutination (Latex) Test: For S. aureus Identification 57 Tube Agglutination Test: The Heterophile Antibody Test 58 White Blood Cell Study: The Differential WBC Count 59 Blood Grouping 60 A Synthetic Epidemic Appendixes A Tables B Indicators, Stains, Reagents C Media D Identification Charts E The Streptococci: Classification, Habitat, Pathology, and Biochemical Characteristics