Gene Cloning and DNA Analysis

Preface to the Eighth Edition

Part I The Basic Principles of Gene Cloning and DNA Analysis

1 Why Gene Cloning and DNA Analysis are Important

2 Vectors for Gene Cloning: Plasmids and Bacteriophages

3 Purification of DNA from Living Cells

4 Manipulation of Purified DNA

5 Introduction of DNA into Living Cells

6 Cloning Vectors for Escherichia coli

7 Cloning Vectors for Eukaryotes

8 How to Obtain a Clone of a Specific Gene

9 The Polymerase Chain Reaction

Part II The Applications of Gene Cloning and DNA Analysis in Research

10 Sequencing Genes and Genomes

11 Studying Gene Expression and Function

12 Studying Genomes

Part III The Applications of Gene Cloning and DNA Analysis in Biotechnology

13 Studying Transcriptomes and Proteomes

14 Production of Protein from Cloned Genes

15 Gene Cloning and DNA Analysis in Medicine

16 Gene Cloning and DNA Analysis in Agriculture

17 Gene Cloning and DNA Analysis in Forensic Science and Archaeology

Glossary

Index