Breast Cancer Research Protocols

Preparation of Cell and Tissue Samples.- Breast Tissue Banking.- Frozen Biopsy Collection and Storage.- Serial Biopsies/Fine-Needle Aspirates and Their Assessment.- Breast Tissue Microarrays.- Preparation of Tumor Homogenates for Subsequent Preparation of Cytosols, Membrane Fractions, RNA, and DNA.- Isolation of RNA From Tumor Samples.- Isolation of DNA From Tumor Samples.- Laser-Assisted Microdissection and Isolation of DNA and RNA.- Markers of Clinical Outcome and Prognosis.- Traditional and Established Indicators of Prognosis and Treatment Success.- Sentinel Lymph Node Biopsy in Breast Cancer.- Pathological Evaluation of Axillary Sentinel Lymph Nodes in Breast Cancer.- Measurement of Estrogen Receptor Status by Immunocytochemistry in Paraffin Wax Sections.- Markers of Apoptosis.- Quantitative Angiogenesis in Breast Cancer.- Analysis of Tumor-Derived Proteins and Antigens.- Immunohistochemistry.- Detection of Aberrant Glycosylation in Breast Cancer Using Lectin Histochemistry.- SDS-PAGE and Western Blotting to Detect Proteins and Glycoproteins of Interest in Breast Cancer Research.- Breast Cancer Proteomics Using Two-Dimensional Electrophoresis.- Procedures for the Quantitative Protein Determination of Urokinase and Its Inhibitor, PAI-1, in Human Breast Cancer Tissue Extracts by ELISA.- Analysis of Genes and Gene Expression in Tumor Specimens.- Fluorescence In Situ Hybridization and Comparative Genomic Hybridization.- Fluorescence In Situ Hybridization Assessment of c-myc Gene Amplification in Breast Tumor Tissues.- Detection of HER2 Gene Amplification by Fluorescence In Situ Hybridization in Breast Cancer.- In Situ Hybridization Combined With Immunohistochemistry to Localize Gene Expression.- Quantitation of RNA by Ribonuclease Protection Assay.- Identification of Steroid Hormone-Regulated Genes in Breast Cancer.- Sequencing of the Tumor Suppressor Gene TP 53.- Expression Profiling Using cDNA Microarrays.- Gene Expression Analysis Using Filter cDNA Microarrays.- Studying Cancer Cell Behavior in Vitro and In Vivo.- Methods to Analyze the Effects of the Urokinase System on Cancer Cell Adhesion, Proliferation, Migration, and Signal Transduction Events.- Phospho-Specific Antibodies as a Tool to Study In Vivo Regulation of BRCA1 After DNA Damage.- Models of Hormone Resistance In Vitro and In Vivo.- Generation of Genetically Modified Embryonic Stem Cells for the Development of Knockout Mouse Animal Model Systems.- In Vivo Xenograft Models of Breast Cancer Metastasis.- Neoadjuvant Endocrine Therapy Models.- Primary Mouse Endothelial Cell Culture for Assays of Angiogenesis.