The application of micro-autoradiographic techniques to ecological studies

I. Synopsis 2
Zusammenfassung 2
II. Introduction 2
III. Comparison of autoradiography with conventional methods for assay of radioactive isotopes 3
IV. Preparation of specimens 4
A. Isotope incorporation 4
B. Removal of excess radioactivity 6
C. Biochemical extraction procedures 7
V. Preparation of slides 8
VI. Liquid emulsion method 10
A. Preparation of emulsion 11
B. Coating the slides 11
C. Storage of slides 14
D. Developing 14
E. Microscopic observation 16
VII. Stripping film method 16
A. Applying the emulsion 17
B. Comparison of liquid emulsion and stripping film techniques. 17
VIII. Evaluation of autoradiograms 18
A. Qualitative evaluation 18
B. Relative quantitation 19
1. Counting grains 19
2. Scoring cells 21
C. Absolute quantitation 25
IX. Limitations of autoradiography 25
X. Summary of autoradiographic methods 25
XI. Acknowledgements 26
References 26