
The application of micro-autoradiographic techniques to ecological studies
E. Schweizerbart (Publisher)
Published on 24. July 1968
Book
Paperback/Softback
29 pages
978-3-510-52015-2 (ISBN)
More details
Series
Language
English
Illustrations
2
6 Schaubilder, 3 s/w Tabellen, 2 s/w Bildtafeln
Dimensions
Height: 240 mm
Width: 160 mm
Weight
80 gr
ISBN-13
978-3-510-52015-2 (9783510520152)
Schweitzer Classification
Content
I. Synopsis 2
Zusammenfassung 2
II. Introduction 2
III. Comparison of autoradiography with conventional methods for assay of radioactive isotopes 3
IV. Preparation of specimens 4
A. Isotope incorporation 4
B. Removal of excess radioactivity 6
C. Biochemical extraction procedures 7
V. Preparation of slides 8
VI. Liquid emulsion method 10
A. Preparation of emulsion 11
B. Coating the slides 11
C. Storage of slides 14
D. Developing 14
E. Microscopic observation 16
VII. Stripping film method 16
A. Applying the emulsion 17
B. Comparison of liquid emulsion and stripping film techniques. 17
VIII. Evaluation of autoradiograms 18
A. Qualitative evaluation 18
B. Relative quantitation 19
1. Counting grains 19
2. Scoring cells 21
C. Absolute quantitation 25
IX. Limitations of autoradiography 25
X. Summary of autoradiographic methods 25
XI. Acknowledgements 26
References 26
Zusammenfassung 2
II. Introduction 2
III. Comparison of autoradiography with conventional methods for assay of radioactive isotopes 3
IV. Preparation of specimens 4
A. Isotope incorporation 4
B. Removal of excess radioactivity 6
C. Biochemical extraction procedures 7
V. Preparation of slides 8
VI. Liquid emulsion method 10
A. Preparation of emulsion 11
B. Coating the slides 11
C. Storage of slides 14
D. Developing 14
E. Microscopic observation 16
VII. Stripping film method 16
A. Applying the emulsion 17
B. Comparison of liquid emulsion and stripping film techniques. 17
VIII. Evaluation of autoradiograms 18
A. Qualitative evaluation 18
B. Relative quantitation 19
1. Counting grains 19
2. Scoring cells 21
C. Absolute quantitation 25
IX. Limitations of autoradiography 25
X. Summary of autoradiographic methods 25
XI. Acknowledgements 26
References 26