Modern Experimental Biochemistry

I. THEORY AND EXPERIMENTAL TECHNIQUES. 1. Introduction to the Biochemistry Laboratory. 2. General Laboratory Procedures. 3. Separation and Purification of Biomolecules by Chromatography. 4. Characterization of Proteins and Nucleic Acids byElectrophoresis. 5. Spectroscopic Analysis of Biomolecules. 6. Radioisotopes in Biochemical Research. 7. Centrifugation of Biomolecules. II. EXPERIMENTS. 1. Using the Biochemical Literature. 2. Analytical Methods for Amino Acid Separation andIdentification. 3. Purification and Characterization of a-Lactalbumin, a MilkProtein. 4. Structural Analysis of a Dipeptide or Tripeptide. 5. Protein Ligand Interactions: Binding of Coomassie Blue Dye toOvalbumin. 6. Kinetic Analysis of an Enzyme, Tyrosinase. 7. Purification and Characterization of a Lipid Extracted fromNutmeg. 8. Fatty Acid Content of Triacylglycerols in Natural Oils. 9. Identification of Carbohydrates by Polarimetry. 10. Characterization of Hemoglobin A1C by Affinity Chromatography. 11. Isolation, Separation and Spectrophotometric Characterization ofPhotosynthetic Pigments. 12. Photoinduced Proton Transport through Chloroplast Membranes. 13. Isolation, Subfractionation and Enzymatic Analysis of Beef HeartMitochondria. 14. Cholesterol (Total and HDL) and Uric Acid Content of BloodSerum: Enzymes as Reagents in Clinical Chemistry. 15. Vitamin C Content of Fruits, Vegetables and Other Foods. 16. Activity and Thermal Stability of Acrylamide Gel-ImmobilizedPeroxidase: An Experiment in Biotechnology. 17. Extraction of Chromosomal DNA from Bacterial Cells. 18. Characterization of Nucleic Acids by Fluorescence of BoundEthidium Bromide. 19. Preparation, Isolation and Purification of Bacterial PlasmidDNA. 20. Rapid, Microscale Isolation of Plasmid DNA. 21. The Action of Restriction Endonucleases on Plasmid or Viral DNA. 22. Specific Cleavage of an Oligomeric Ribonucleotide Substrate by aRibozyme (RNA Enzyme). Appendices: I. Properties of Common Acids and Bases. II. Properties of Common Buffer Compounds. III. pKa Values and pHI Values of Amino Acids. IV. Molecular Weights of Some Common Proteins. V. Common Abbreviations Used in This Text. VI. Units of Measurement. VII. Table of the Elements. VIII. Values of t for Analysis of Statistical Confidence Limits. IX. Solutions to Selected Questions.