Microbiological Applications: A Laboratory Manual in General Microbiology, Complete Version

Part 1 Microscopy1 Brightfield Microscopy2 Darkfield Microscopy3 Phase-Contrast Microscopy4 Fluorescence Microscopy 5 Microscopic MeasurementsPart 2 Survey of Microorganisms6 Protozoa, Algae, and Cyanobacteria7 Microscopic Invertebrates8 Aseptic Technique 9 The Bacteria10 The Fungi: Yeasts and MoldsPart 3 Microscope Slide Techniques11 Negative Staining12 Smear Preparation13 Simple Staining14 Capsular Staining15 Gram Staining16 Spore Staining: Two Methods17 Acid-Fast Staining: Ziehl-Neelsen Method18 Acid-Fast Staining: Fluorescence Method19 Motility DeterminationPart 4 Culture Methods20 Culture Media Preparation21 Pure Culture Techniques22 Cultivation of Anaerobes23 Bacterial Population Counts24 Slide Culture: Autotrophs25 Slime Mold Culture 26 Slide Culture: Molds 27 Isolation of Anaerobic Phototrophic Bacteria: Using the Winogradsky ColumnPart 5 Bacterial Viruses28 Isolation of Phage from Sewage29 Isolation of Phage from Flies30 Burst Size Determination: A One-Step Growth CurvePart 6 Microbial Interrelationships31 Bacterial Commensalism32 Bacterial Synergism33 Microbial AnatagonismPart 7 Environmental Influences and Control of Microbial Growth34 Temperature: Effects on Growth35 Temperature: Lethal Effects36 pH and Microbial Growth37 Osmotic Pressure and Bacterial Growth38 Oligodynamic Action39 Ultraviolet Light: Lethal Effects40 Evaluation of Disinfectants: The Use-Dilution Method41 Evaluation of Alcohol: Its Effectiveness as a Skin Degerming Agent42 Evaluation of Antiseptics: The Filter Paper Disk Agent43 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method44 Effectiveness of Hand ScrubbingPart 8 Identification of Unknown Bacteria45 Preparation and Care of Stock Cultures46 Morphological Study of Unknown47 Cultural Characteristics48 Physiological Characteristics: Oxidation and Fermentation Tests49 Physiological Characteristics: Hydrolytic Reactions50 Physiological Characteristics: Miscellaneous Tests51 Use of Bergey's Manual and Identibacter InteractusPart 9 Miniaturized Multitest Systems52 Enterobacteriaceae Identification: The API 20E System53 Enterobacteriaceae Identification: The Enterotube II System54 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System55 Staphylococcus Identification: The API Staph-Ident SystemPart 10 Microbiology of Soil56 Microbial Population Counts of Soil57 Isolation of an Antibiotic Producer from Soil58 The Nitrogen Cycle59 Nitrogen-Fixing Bacteria60 Ammonification in Soil61 Isolation of a Denitrifier from Soil: Using Nitrate Agar62 Isolation of a Denitrifier from Soil: Using an Enrichment MediumPart 11 Microbiology of Water63 Bacteriological Examination of Water: Qualitative Tests64 The Membrane Filter Method65 Standard Plate Count: A Quantitative TestPart 12 Microbiology of Milk and Food Products66 Standard Plate Count of Milk67 Direct Microscopic Count of Organisms in Milk: The Breed Count68 The Reductase Test69 Bacterial Counts of Foods70 Microbial Spoilage of Canned Foods71 Microbial Spoilage of Refrigerated Meat72 Microbiology of Alcohol Fermentation 73 Microbiology of Yogurt ProductionPart 13 Bacterial Genetic Variations74 Mutant Isolation of Gradient Plate Method75 Mutant Isolation by Replica Plating76 Bacterial Mutagenicity and Carcinogenesis: The Ames TestPart 14 Medical Microbiology and Immunology77 A Synthetic Epidemic78 The Staphylococci: Isolation and Identification79 The Streptococci: Isolation and Identification80 Gram-Negative Intestinal Pathogens81 Urinary Tract Pathogens82 Slide Agglutination Test: Serological Typing83 Slide Agglutination (Latex) Test: For S. aureus Identification84 Tube Agglutination Test: The Heterophile Antibody Test85 Tube Agglutination Test: The Widal Test86 Phage Typing87 White Blood Cell Study: The Differential WBC Count88 Total WBC Count89 Blood Grouping90 The Snyder Caries Susceptibility TestAppendix A TablesAppendix B Indicators, Stains, ReagentsAppendix C MediaAppendix D Identificaiton ChartsAppendix E The StreptococciAppendix F Identibacter interactus