Microbiological Applications: Complete Version

Part 1. Microscopy. 1. Brightfield Microscopy. 2. Darkfield Microscopy. 3. Phase-Contrast Microscopy. 4. Fluorescence Microscopy. 5. Microscopic Measurements. Part 2. Survey of Microorganisms. 6. Protozoa, Algae, and Cyanobacteria. 7. Microscopic Invertebrates. 8. Aseptic Technique. 9. The Bacteria. 10. The Fungi: Yeasts and Molds. Part 3. Microscope Slide Techniques. 11. Negative Staining. 12. Smear Preparation. 13. Simple Staining. 14. Capsular Staining. 15. Gram Staining. 16. Spore Staining: Two Methods. 17. Acid-Fast Staining: Ziehl-Neelsen Method. 18. Acid-Fast Staining: Fluorescence Method. 19. Motility Determination. Part 4. Culture Methods. 20. Culture Media Preparation. 21. Pure Culture Techniques. 22. Cultivation of Anaerobes. 23. Bacterial Population Counts. 24. Slide Culture: Autotrophs. 25. Slime Mold Culture. 26. Slide Culture: Molds. 27. Isolation of Anaerobic Phototrophic Bacteria: Using the Winogradsky Column. Part 5. Bacterial Viruses. 28. Isolation of Phage from Sewage. 29. Isolation of Phage from Flies. 30. Burst Size Determination: A One-Step Growth Curve. Part 6. Microbial Interrelationships. 31. Bacterial Commensalism. 32. Bacterial Synergism. 33. Microbial Anatagonism. Part 7. Environmental Influences and Control of Microbial Growth. 34. Temperature: Effects on Growth. 35. Temperature: Lethal Effects. 36. pH and Microbial Growth. 37. Osmotic Pressure and Bacterial Growth. 38. Oligodynamic Action. 39. Ultraviolet Light: Lethal Effects. 40. Evaluation of Disinfectants: The Use-Dilution Method. 41. Evaluation of Alcohol: Its Effectiveness as a Skin Degerming Agent. 42. Evaluation of Antiseptics: The Filter Paper Disk Agent. 43. Antimicrobic Sensitivity Testing: The Kirby-Bauer Method. 44. Effectiveness of Hand Scrubbing. Part 8. Identification of Unknown Bacteria. 45. Preparation and Care of Stock Cultures. 46. Morphological Study of Unknown. 47. Cultural Characteristics. 48. Physiological Characteristics: Oxidation and Fermentation Tests. 49. Physiological Characteristics: Hydrolytic Reactions. 50. Physiological Characteristics: Miscellaneous Tests. 51. Use of Bergey's Manual and Identibacter Interactus. Part 9. Miniaturized Multitest Systems. 52. Enterobacteriaceae Identification: The API 20E System. 53. Enterobacteriaceae Identification: The Enterotube II System. 54. O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System. 55. Staphylococcus Identification: The API Staph-Ident System. Part 10. Microbiology of Soil. 56. Microbial Population Counts of Soil. 57. Isolation of an Antibiotic Producer from Soil. 58. The Nitrogen Cycle. 59. Nitrogen-Fixing Bacteria. 60. Ammonification in Soil. 61. Isolation of a Denitrifier from Soil: Using Nitrate Agar. 62. Isolation of a Denitrifier from Soil: Using an Enrichment Medium. Part 11. Microbiology of Water. 63. Bacteriological Examination of Water: Qualitative Tests. 64. The Membrane Filter Method. 65. Standard Plate Count: A Quantitative Test. Part 12. Microbiology of Milk and Food Products. 66. Standard Plate Count of Milk. 67. Direct Microscopic Count of Organisms in Milk: The Breed Count. 68. The Reductase Test. 69. Bacterial Counts of Foods. 70. Microbial Spoilage of Canned Foods. 71. Microbial Spoilage of Refrigerated Meat. 72. Microbiology of Alcohol Fermentation. 73. Microbiology of Yogurt Production. Part 13. Bacterial Genetic Variations. 74. Mutant Isolation of Gradient Plate Method. 75. Mutant Isolation by Replica Plating. 76. Bacterial Mutagenicity and Carcinogenesis: The Ames Test. Part 14. Medical Microbiology and Immunology. 77. A Synthetic Epidemic. 78. The Staphylococci: Isolation and Identification. 79. The Streptococci: Isolation and Identification. 80. Gram-Negative Intestinal Pathogens. 81. Urinary Tract Pathogens. 82. Slide Agglutination Test: Serological Typing. 83. Slide Agglutination (Latex) Test: For S. aureus Identification. 84. Tube Agglutination Test: The Heterophile Antibody Test. 85. Tube Agglutination Test: The Widal Test. 86. Phage Typing. 87. White Blood Cell Study: The Differential WBC Count. 88. Total WBC Count. 89. Blood Grouping. 90. The Snyder Caries Susceptibility Test. Appendix A. Tables. Appendix B. Indicators, Stains, Reagents. Appendix C. Media. Appendix D. Identification Charts. Appendix E. The Streptococci. Appendix F. Identibacter interactus.