Benson's Microbiological Applications: Complete Version

Preface Laboratory Protocol Safety Part 1 Microscopy 1 Brightfield Microscopy 2 Darkfield Microscopy 3 Phase-Contrast Microscopy 4 Fluorescence Microscopy 5 Microscopic Measurements Part 2 Survey of Microorganisms 6 Protozoa, Algae, and Cyanobacteria 7 Ubiquity of Bacteria 8 Fungi and Yeasts Part 3 Manipulation of Microorganisms 9 Aseptic and Pure Culture Techniques 10 The Streak Plate 11 The Pour Plate Part 4 Staining and Observation of Microorganisms 12 Smear Preparation 13 Simple Stain 14 Negative Stain 15 Capsular Stain 16 Gram Staining 17 Spore Stain 18 Acid-Fast Stain: Ziehl-Neelsen 19 Observation of Motility Part 5 Culture Methods 20 Media Preparation 21 Preparation of Stock Cultures 22 Cultivation of Anaerobic Bacteria 23 Enumeration of Bacteria: The Standard Plate Count 24 Cultivation of Slime Molds 25 Slide Culture: Fungi Part 6 Bacterial Viruses 26 Phage Plaque Assay 27 Burst Size Determination 28 Isolation of Phage from Flies 29 Phage Typing of Bacteria Part 7 Control of Microbial Growth 30 Temperature: Effects on Growth 31 Temperature: Lethal Effects on Growth 32 Effects of pH on Growth 33 Effects of Osmotic Pressure 34 Effects of UV Light 35 Oligodynamic Action of Metals 36 Effectiveness of Alcohol as an Antiseptic 37 Testing Antibiotics and Antimicrobials: The Kirby-Bauer Technique 38 Evaluating Disinfectants and Antiseptics: The Filter Paper Disk Method 39 Effectiveness of Hand Washing Part 8 Identification of Unknown Bacteria 40 Morphological Study of Unknown 41 Cultural Characteristics 42 Physiological Characteristics: Oxidation and Fermentation Reactions 43 Physiological Characteristics: Biochemical Tests 44 Bergey's Manual: Identification of an Unknown Bacterium Part 9 Miniaturized Multitest Systems for Bacterial Identification 45 API 20E System for Enterobacteriaceae 46 Enterotube II System for Enterobacteriaceae 47 The Oxi/Ferm Tube II System for Gram Negative Rods 48 API StaphIdent System for Staphylococcus Part 10 Microbial Diversity and Environmental Microbiology 49 Isolation of Streptomyces: Antibiotic Production The Nitrogen Cycle 50 Ammonia Oxidation in the Soil 51 Rhizobium: Sumbiotic Nitrogen Fixation 52 Azotobacter: Free-living Nitrogen Fixation 53 Paracoccus denitrifcans: Denitrifcation The Sulfur Cycle: The Winogradsky Column 54 Photosynthetic and Chemolithotrophic Bacteria 55 Desulfovibrio: Anaerobic Sulfur Respiration Microbila Interrelationships 56 Commensalism 57 Synergism 58 Microbial Antagonism Part 11 Applied Microbiology 59 Determining the Number of Bacteria in Food 60 Bacterial Examination of Water: MPN Determination of Coliforms 61 Membrane Filter Technique for Coliform Determination 62 Reductase Test for Milk 63 Microbial Spoilage of Canned Food 64 Microbiology of Alcohol Fermentation Part 12 Bacterial Genetics and Biotechnology 65 Isolation by Replica Plating 66 Bacterial Transformation 67 Electrophoresis of DNA: Isolation of Plasmids 68 PCR: The Polymerase Chain Reaction for Amplifying DNA Part 13 Medical Microbiology and Serology The Gram Positive Cocci 69 Staphyloccus 70 Streptococcus 71 Gram Negative Intestinal Pathogens 72 Slide Agglutination Test for Salmonella 73 Slide Agglutination Test for Staphyloccus 74 Tube Agglutination: The Heterophile Antibody Test 75 Differential White Blood Cell Count 76 Blood Typing: ABO and Rh Blood Groups 77 Simulated Epidemic Descriptive Charts Appendix A Tables Appendix B Stains and Reagents Appendix C Media Appendix D Identification Charts Appendix E The Streptococci References Index