CRISPR: Biology and Applications

Since their discovery and subsequent development into laboratory tools, CRISPR-Cas systems have revolutionized the science of gene editing and their possible applications continue to expand, from basic research to potentially groundbreaking medical and commercial uses. Led by a distinguished team of editors, CRISPR: Biology and Applications explores the subject matter needed to delve into this fascinating area. This comprehensive text presents the diversity of CRISPR-Cas systems, the underlying biology of these systems, and CRISPR-based technologies and applications. Topics covered include: * Classification and molecular mechanisms of CRISPR-Cas systems * CRISPR-Cas evolution, regulation, expression, and function * Uses for gene editing and modulation of gene expression * CRISPR-based antimicrobials and phage resistance for medical and industrial purposes Written by internationally renowned authors, CRISPR: Biology and Applications serves as both an introductory guide for those new to the field and a ready reference for seasoned researchers whose work touches this evolving and headline-making science. "An excellent compendium of all things CRISPR from some of the leading minds in the field. With thorough coverage from every angle and beautifully detailed illustrations, this book is not to be missed!"--Jennifer A. Doudna, Professor of Chemistry, Biochemistry & Molecular Biology, UC Berkeley; Founder, Innovative Genomics Institute; Nobel Laureate and coinventor of CRISPR technology "This journey through CRISPR biology and several of its breakthrough applications offers an exciting glimpse into one of the most beautiful and compelling fields in the life sciences."--David R. Liu, Director of the Merkin Institute at the Broad Institute of MIT and Harvard; Professor of Chemistry and Chemical Biology at Harvard University; coinventor of base editing and prime editing "A must read! The CRISPR topics, written by world-leading experts, span from the fascinating mechanistic underpinnings to the ingenious applications. Students and scientists who want to delve into biology's most timely topic will not be able to put this book down. Each chapter is easy to read and comes with especially helpful diagrams. One can read from start to finish or pick and choose themes. Either way, the book delivers utterly enjoyable learning!" --Bonnie Bassler, Professor, Princeton University; Investigator, Howard Hughes Medical Institute Rodolphe Barrangou is the Todd R. Klaenhammer Distinguished Professor in Probiotics Research in the Department of Food, Bioprocessing and Nutrition Sciences at North Carolina State University; cofounder and CEO of CRISPR Biotechnologies; cofounder and CSO of Ancilia Biosciences; cofounder, President, and CSO of TreeCo; cofounder and member of the Scientific Advisory Board of Intellia Therapeutics; and Editor-in-Chief of The CRISPR Journal. Erik J. Sontheimer is Professor in the RNA Therapeutics Institute and the Program for Molecular Medicine at the University of Massachusetts Medical School; an Associate Editor of RNA; and cofounder and a member of the Scientific Advisory Board at Intellia Therapeutics. Luciano A. Marraffini is the Kayden Family Professor and Head of the Laboratory of Bacteriology at The Rockefeller University; a Howard Hughes Medical Institute Investigator; a Rita Allen Foundation Scholar; a Searle Scholar, and a recipient of the NIH Director's New Innovator and Pioneer Awards.