Regulators and Effectors of Small GTPases, Part E: GTPases Involved in Vesicular Traffic: Volume 329
Published on 8. February 2001
Book
Hardback
553 pages
978-0-12-182230-9 (ISBN)
Description
Small GTPases play a key role in many aspects of contemporary cell biology: control of cell growth and differentiation; regulation of cell adhesion and cell movement; the organization of the actin cytoskeleton; and the regulation of intracellular vesicular transport.
This volume and its companions (Volumes 255, 256, 257, and the forthcoming 325) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.
This volume and its companions (Volumes 255, 256, 257, and the forthcoming 325) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.
More details
Series
Language
English
Place of publication
San Diego
United States
Publishing group
Elsevier Science Publishing Co Inc
Target group
Professional and scholarly
Biochemists, molecular biologists, cell biologists, pharmacologists, neurophysiologists, and neurochemists.
Dimensions
Height: 229 mm
Width: 152 mm
Weight
910 gr
ISBN-13
978-0-12-182230-9 (9780121822309)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Persons
Content
Section I. Rab GTPases
1: Structural Basis for Rab Function: An Overview
2: Expression of Wild-Type and Mutant Green Fluorescent Protein-Rabl for Fluorescence Microscopy Analysis
3: Fluorescence Methods for Monitoring Interactions of Rab Proteins with Nucleotides, Rab Escort Protein, and Geranylgeranyltransferase
4: Prenylation of Rab Proteins in Vitro by Geranylgeranyltransferases
5: Antibody and Oligonucleotide Probes to Distinguish Intracellular Expression and Localization Patterns of Rab GDP-Dissociation Inhibitor Isoforms
6: Expression, Purification, and Biochemical Properties of Ypt/Rab GTPase-Activating Proteins of Gyp Family
7: Purification and Properties of Rab3 GDP/GTP Exchange Protein
8: Purification and Properties of Rab3 GTPase-Activating Protein
9: Rabphilin-3: A Target Molecule for Rab3 Small G Proteins
10: Doc2I+/- as Modulator of Ca2+-Dependent Exocytosis
11: Purification and Properties of a GTPase-Activating Protein for Yeast Rab GTPases
12: Purification and Characterization of Yeast Exocyst Complex
13: Expression and Properties of Rab4 and Its Effector Rabaptin-4 in Endocytic Recycling
14: Purification of EEA1 from Bovine Brain Cytosol Using Rab5 Affinity Chromatography and Activity Assays
15: Expression, Purification, and Characterization of Rab5 Effector Complex, Rabaptin-5/Rabex-5
16: Measurement of Rab5 Protein Kinase B/akt and Regulation of Ras-Activated Endocytosis
17: Expression, Purification, and Biochemical Properties of Rabkinesin-6 Domains and Their Interactions with Rab6A
18: Expression and Functional Analyses of Rab8 and Rablla in Exocytic Transport from trans-Golgi Network
19: Expression and Properties of Rab7 in Endosome Function
20: Expression, Purification, and Properties of Rab8 Function in Actin Cortical Skeleton Organization and Polarized Transport
21: Properties of Rab13 Interaction with Rod cGMP Phosphodiesterase I? Subunit
22: Subcellular Localization of Rab17 by Cryo-Immunogold Electron Microscopy in Epithelial Cells Grown on Polycarbonate Filters
23: Expression and Properties of Rab25 in Polarized Madin-Darby Canine Kidney Cells
24: Purification of TRAPP from Saccharomyces cerevisiae and Identification of Its Mammalian Counterpart
Section II. ADP-Ribosylation Factor (ARF) GTPases
25: Structural and Functional Organization of ADP-Ribosylation Factor (ARF) Proteins
26: Expression and Properties of ADP-Ribosylation Factor (ARF6) in Endocytic Pathways
27: Expression and Analysis of ARNO and ARNO Mutants and Their Effects on ADP-Ribosylation Factor (ARF)-Mediated Actin Cytoskeletal Rearrangements
28: Expression, Purification, and Measurements of Activity of ARNO1, a Guanine Nucleotide Exchange Factor for ADP-Ribosylation Factor 1 (ARF1)
29: Expression, Purification, and Biochemical Properties of EFA6, a Sec7 Domain-Containing Guanine Exchange Factor for ADP-Ribosylation Factor 6 (ARF6)
30: Isolation and Properties of GRP1, an ADP-Ribosylation Factor (ARF)-Guanine Nucleotide Exchange Protein Regulated by Phosphatidylinositol 3, 4, 5-Trisphosphate
31: Functional Analysis of ADP-Ribosylation Factor (ARF) Guanine Nucleotide Exchange Factors Gea1p and Gea2p in Yeast
32: Isolation, Cloning, and Characterization of Brefeldin A-Inhibited Guanine Nucleotide-Exchange Protein for ADP-Ribosylation Factor
33: Expression, Purification, and Properties of ADP-Ribosylation Factor (ARF) GTPase Activating Protein-1
34: Expression, Analysis, and Properties of Yeast ADP-Ribosylation Factor (ARF) GTPase Activating Proteins (GAPs) Gcs1 and Glo3
35: Purification and Properties of ARD1, An ADP-Ribosylation Factor (ARF)-Related Protein with GTPase-Activating Domain
36: Purification and Characterization of GIT Family of ADP-Ribosylation Factor (ARF) GTPase-Activating Proteins
37: Assay and Purification of Phosphoinositide-Dependent ADP-Ribosylation Factor (ARF) GTPase Activating Proteins
38: Biological Properties and Measurement of Phospholipase D Activation by ADP-Ribosylation Factor (ARF)
39: Use of Aminoglycoside Antibiotics and Related Compounds to Study ADP-Ribosylation Factor (ARF) /Coatomer Function in Golgi Traffic
40: Adaptor Protein 1-Dependent Clathrin Coat Assembly on Synthetic Liposomes and Golgi Membranes
41: Receptor-Dependent Formation of COPI-Coated Vesicles from Chemically Defined Donor Liposomes
42: ADP-Ribosylation Factor (ARF) as Regulator of Spectrin Assembly at Golgi Complex
43: Purification, Properties, and Analysis of yARL3
44: Preparation and Assay of Recombinant ADP-Ribosylation Factor-Like Protein-1 (ARL1)
Section III. Sar GTPases
45: Purification and Properties of Rat Liver Sec23aEUR"Sec24 Complex
46: Purification of Functional Sec13paEUR"Sec31p Complex, A Subunit of COPII Coat
Section IV. Dynamin GTPases
47: Expression, Purification, and Functional Assays for Self-Association of Dynamin-1
48: Analysis of Phosphoinositide Binding by Pleckstrin Homology Domain from Dynamin
49: Mapping Dynamin Interdomain Interactions with Yeast Two-Hybrid and Glutathione S-Transferase Pulldown Experiments
50: Interactions of Dynamin and Amphiphysin with Liposomes
51: Activation of Dynamin GTPase Activity by Phosphoinositides and SH3 Domain-Containing Proteins
Section V. Septin GTPases
52: Expression and Analysis of Properties of Septin CDCrel-1 in Exocytosis
Author Index
Subject Index
1: Structural Basis for Rab Function: An Overview
2: Expression of Wild-Type and Mutant Green Fluorescent Protein-Rabl for Fluorescence Microscopy Analysis
3: Fluorescence Methods for Monitoring Interactions of Rab Proteins with Nucleotides, Rab Escort Protein, and Geranylgeranyltransferase
4: Prenylation of Rab Proteins in Vitro by Geranylgeranyltransferases
5: Antibody and Oligonucleotide Probes to Distinguish Intracellular Expression and Localization Patterns of Rab GDP-Dissociation Inhibitor Isoforms
6: Expression, Purification, and Biochemical Properties of Ypt/Rab GTPase-Activating Proteins of Gyp Family
7: Purification and Properties of Rab3 GDP/GTP Exchange Protein
8: Purification and Properties of Rab3 GTPase-Activating Protein
9: Rabphilin-3: A Target Molecule for Rab3 Small G Proteins
10: Doc2I+/- as Modulator of Ca2+-Dependent Exocytosis
11: Purification and Properties of a GTPase-Activating Protein for Yeast Rab GTPases
12: Purification and Characterization of Yeast Exocyst Complex
13: Expression and Properties of Rab4 and Its Effector Rabaptin-4 in Endocytic Recycling
14: Purification of EEA1 from Bovine Brain Cytosol Using Rab5 Affinity Chromatography and Activity Assays
15: Expression, Purification, and Characterization of Rab5 Effector Complex, Rabaptin-5/Rabex-5
16: Measurement of Rab5 Protein Kinase B/akt and Regulation of Ras-Activated Endocytosis
17: Expression, Purification, and Biochemical Properties of Rabkinesin-6 Domains and Their Interactions with Rab6A
18: Expression and Functional Analyses of Rab8 and Rablla in Exocytic Transport from trans-Golgi Network
19: Expression and Properties of Rab7 in Endosome Function
20: Expression, Purification, and Properties of Rab8 Function in Actin Cortical Skeleton Organization and Polarized Transport
21: Properties of Rab13 Interaction with Rod cGMP Phosphodiesterase I? Subunit
22: Subcellular Localization of Rab17 by Cryo-Immunogold Electron Microscopy in Epithelial Cells Grown on Polycarbonate Filters
23: Expression and Properties of Rab25 in Polarized Madin-Darby Canine Kidney Cells
24: Purification of TRAPP from Saccharomyces cerevisiae and Identification of Its Mammalian Counterpart
Section II. ADP-Ribosylation Factor (ARF) GTPases
25: Structural and Functional Organization of ADP-Ribosylation Factor (ARF) Proteins
26: Expression and Properties of ADP-Ribosylation Factor (ARF6) in Endocytic Pathways
27: Expression and Analysis of ARNO and ARNO Mutants and Their Effects on ADP-Ribosylation Factor (ARF)-Mediated Actin Cytoskeletal Rearrangements
28: Expression, Purification, and Measurements of Activity of ARNO1, a Guanine Nucleotide Exchange Factor for ADP-Ribosylation Factor 1 (ARF1)
29: Expression, Purification, and Biochemical Properties of EFA6, a Sec7 Domain-Containing Guanine Exchange Factor for ADP-Ribosylation Factor 6 (ARF6)
30: Isolation and Properties of GRP1, an ADP-Ribosylation Factor (ARF)-Guanine Nucleotide Exchange Protein Regulated by Phosphatidylinositol 3, 4, 5-Trisphosphate
31: Functional Analysis of ADP-Ribosylation Factor (ARF) Guanine Nucleotide Exchange Factors Gea1p and Gea2p in Yeast
32: Isolation, Cloning, and Characterization of Brefeldin A-Inhibited Guanine Nucleotide-Exchange Protein for ADP-Ribosylation Factor
33: Expression, Purification, and Properties of ADP-Ribosylation Factor (ARF) GTPase Activating Protein-1
34: Expression, Analysis, and Properties of Yeast ADP-Ribosylation Factor (ARF) GTPase Activating Proteins (GAPs) Gcs1 and Glo3
35: Purification and Properties of ARD1, An ADP-Ribosylation Factor (ARF)-Related Protein with GTPase-Activating Domain
36: Purification and Characterization of GIT Family of ADP-Ribosylation Factor (ARF) GTPase-Activating Proteins
37: Assay and Purification of Phosphoinositide-Dependent ADP-Ribosylation Factor (ARF) GTPase Activating Proteins
38: Biological Properties and Measurement of Phospholipase D Activation by ADP-Ribosylation Factor (ARF)
39: Use of Aminoglycoside Antibiotics and Related Compounds to Study ADP-Ribosylation Factor (ARF) /Coatomer Function in Golgi Traffic
40: Adaptor Protein 1-Dependent Clathrin Coat Assembly on Synthetic Liposomes and Golgi Membranes
41: Receptor-Dependent Formation of COPI-Coated Vesicles from Chemically Defined Donor Liposomes
42: ADP-Ribosylation Factor (ARF) as Regulator of Spectrin Assembly at Golgi Complex
43: Purification, Properties, and Analysis of yARL3
44: Preparation and Assay of Recombinant ADP-Ribosylation Factor-Like Protein-1 (ARL1)
Section III. Sar GTPases
45: Purification and Properties of Rat Liver Sec23aEUR"Sec24 Complex
46: Purification of Functional Sec13paEUR"Sec31p Complex, A Subunit of COPII Coat
Section IV. Dynamin GTPases
47: Expression, Purification, and Functional Assays for Self-Association of Dynamin-1
48: Analysis of Phosphoinositide Binding by Pleckstrin Homology Domain from Dynamin
49: Mapping Dynamin Interdomain Interactions with Yeast Two-Hybrid and Glutathione S-Transferase Pulldown Experiments
50: Interactions of Dynamin and Amphiphysin with Liposomes
51: Activation of Dynamin GTPase Activity by Phosphoinositides and SH3 Domain-Containing Proteins
Section V. Septin GTPases
52: Expression and Analysis of Properties of Septin CDCrel-1 in Exocytosis
Author Index
Subject Index