Bacterial Regulatory RNA

RNA extraction from Gram-positive bacteria membrane vesicles using a polymer-based precipitation method.- Extraction and purification of outer membrane vesicles and their associated RNAs.- Analysis of phage regulatory RNAs: sequencing library construction from the fraction of small prokaryotic RNAs less than 50 nucleotides in length.- Discovering Novel Bacterial Small RNA by RNA-seq Analysis Toolkit ANNOgesic.- Ribosome profiling methods adapted to the study of RNA-dependent translation regulation in Staphylococcus aureus.- CRISPR interference-based functional small RNA genomics.- Investigation of sRNA-mRNA interactions in Bacillus subtilis in vivo.- In vitro methods for the investigation of sRNA-mRNA interactions in Bacillus subtilis.- RNA double helix hybridization measured by fluorescence correlation spectroscopy.- New perspectives on cross talks between bacterial regulatory RNAs from outer membrane vesicles and eukaryotic cells.- Experimental validation of RNA-RNA interactions by Electrophoretic Mobility Shift Assay.- Dynamics and function of sRNA/mRNAs under the scrutiny of computational simulation methods.-Analysis of sRNAs and their mRNA targets in Sinorhizobium meliloti: focus on half-live determinationEvaluation of 5'-end phosphorylation for small RNA stability and target regulation in vivo.- In-gel cyanoethylation for pseudouridines mass spectrometry detection of bacterial regulatory RNA.- Directed screening for sRNA targets in E. coli using a plasmid library.- Defining Bacterial RNA-RNA Interactomes Using CLASH.- Global identification of RNA-binding proteins in bacteria.- An integrated affinity chromatography-based approach to unravel the sRNA interactome in nitrogen-fixing rhizobia.- sRNA structural modeling based on NMR data.- Circular and linear dichroism for the analysis of small noncoding RNA properties.