Single-Molecule Science
From Super-Resolution Microscopy to DNA Mapping and Diagnostics
Published on 26. May 2022
Book
Hardback
170 pages
978-1-108-42336-6 (ISBN)
Description
Single Molecule Science (SMS) has emerged from developing, using and combining technologies such as super-resolution microscopy, atomic force microscopy, and optical and magnetic tweezers, alongside sophisticated computational and modelling techniques. This comprehensive, edited volume brings together authoritative overviews of these methods from a biological perspective, and highlights how they can be used to observe and track individual molecules and monitor molecular interactions in living cells. Pioneers in this fast-moving field cover topics such as single molecule optical maps, nanomachines, and protein folding and dynamics. A particular emphasis is also given to mapping DNA molecules for diagnostic purposes, and the study of gene expression. With numerous illustrations, this book reveals how SMS has presented us with a new way of understanding life processes. A must-have for researchers and graduate students, as well as those working in industry, primarily in the areas of biophysics, biological imaging, genomics and structural biology.
Reviews / Votes
'Single-molecule experiments have revolutionized our approaches to biophysics and biochemistry at molecular scales. Only a handful of reference textbooks serves the ever-expanding community of practitioners, beginners, and curious amateurs of single-molecule techniques. This volume is poised to become a new single-molecule reference work, to be readily at hand in any bioscience laboratory, to be read and understood by any scientist practicing, or simply interested, in the new world of experiments, reasoning, and statistical analysis opened up by single-molecule science.' Michel A. G. J. Orrit, Professor of Spectroscopy of Molecules in Condensed Matter, Leiden University, The Netherlands 'An excellent collection of cutting-edge reports related to single-molecule spectrometry and imaging that consists of widespread contents from basic to application; both students [and] experts will find an extended application of the technologies to biological and clinical research.' Akira Watanabe, Assistant Professor of Life Science Frontiers, Kyoto University, Japan 'Gives an overview of the applications of single-molecule techniques in the biological sciences. It addresses topics as diverse as super-resolution microscopy (far beyond the Abbe limit) and the study of protein mechanics without ensemble averaging, and, due to its compact size, is informative reading for students and experts alike.' Lothar Kador, Professor of Physics, University of Bayreuth, Germany 'You will learn about the birth of detection of individual biomolecules, associated state-of-the-art technologies, cutting-edge research, and potential medical applications, all from a single-molecule point of view that will inform your new biological thoughts and insights.' Katsuyuki Shiroguchi, Principal Investigator, RIKEN Center for Integrative Medical Sciences, Yokohama, Japan 'This is a thorough introduction to single-molecule techniques and their applications ... Recommended.' M. S. Kainz, ChoiceMore details
Language
English
Place of publication
Cambridge
United Kingdom
Target group
Professional and scholarly
College/higher education
Illustrations
Worked examples or Exercises; 1 Tables, black and white; 6 Halftones, black and white; 28 Line drawings, black and white
Dimensions
Height: 250 mm
Width: 175 mm
Thickness: 14 mm
Weight
484 gr
ISBN-13
978-1-108-42336-6 (9781108423366)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
Additional editions
Krishnarao Appasani | Raghu Kiran Appasani
Single-Molecule Science
From Super-Resolution Microscopy to DNA Mapping and Diagnostics
E-Book
05/2022
Cambridge University Press
€100.99
Available for download
Content
Preface; I. Super Resolution Microscopy and Molecular Imaging Techniques to Probe Biology: 1. Introduction on Single Molecule Science Krishnarao Appasani and Raghu K. Appasani; 2. One molecule, two molecules, red molecules, blue molecules: Methods for quantifying localization microscopy data George Patterson and Gaetan G. Herbomel; 3. Muti-scale fluorescence imaging Ruben Bulkescher, Inn Chung, Jan Philipp Eberle, Holger Erfle, Manuel Gunkel, Juergen Reymann, Karsten Rippe, Guido Sauter, Ronald Simon and Vytaute Starkuviene; 4. Long-read single-molecule optical maps Assaf Grunwald, Yael Michaeli and Yuval Ebenstein; II. Protein Folding, Structure, Confirmation and Dynamics: 5. Single-molecule mechanics of protein nanomachines Gabriel Zoldak and Katarzyna Tych; 6. Post-translational Protein Translocation Through Membranes at the Single Molecule Level Diego Quiroga-Roger, Hilda M. Alfaro-Valdes and Christian A.M. Wilson Moya; III. Mapping DNA Molecules at Single Molecule Level: 7. Observing Dynamic states of single molecule DNA and Proteins using Atomic Force Microscope Jingqiang Li, Sithara Wijeratne and Ching-Hwa Kiang; 8. Atomic Force Microscopy and Detecting a DNA Biomarker of a Few Copies without Amplification Sourav Mishra, Yoonhee Lee and Joon Won Park; IV. Single Molecule Biology to Study Gene Expression: 9. Single molecule detection in the study of gene expression Vipin Kumar, Simon Leclerc and Yuichi Taniguchi.