Amino Acid Analysis
Description
Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method. Another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols presents a broad spectrum of all available methods allowing for readers to choose the method that most suits their particular laboratory set-up and analytical needs. In this volume, a reader can find chapters describing general as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry as well as in food analysis, microbiology, marine biology, drug metabolism, even archeology. Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular BiologyT series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Amino Acid Analysis: Methods and Protocols provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences.
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Content
Rapid LC-MS/MS Profiling of Protein Amino Acids and Metabolically Related Compounds for Large-Scale Assessment of Metabolic Phenotypes.- Combination of an AccQ?Tag-Ultra Performance Liquid Chromatographic Method with Tandem Mass Spectrometry for the Analysis of Amino Acids.- Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry for Quantitative Amino Acid Analysis.- Analysis of Underivatized Amino Acids: Zwitterionic Hydrophilic Interaction Chromatography Combined with Triple Quadrupole Tandem Mass Spectrometry.- Amino Acid Analysis via LC-MS Method after Derivatization with Quaternary Phosphonium.- Amino Acid Analysis by Hydrophilic Interaction Chromatography Coupled with Isotope Dilution Mass Spectrometry.- A Universal HPLC-MS Method to Determine the Stereochemistry of Common and Unusual Amino Acids.- Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry.- New Advances in Amino Acid Profiling by Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry (CE-ESI-MS).- Optimal Conditions for the Direct RP-HPLC Determination of Underivatized Amino Acids with On-line Multiple Detection.- Absolute Quantitation of Proteins by Acid Hydrolysis Combined with Amino Acid Detection by Mass Spectrometry.- Amino Acid Analysis by Means of MALDI TOF Mass Spectrometry or MALDI TOF/TOF Tandem Mass Spectrometry.- Heptafluorobutyl Chloroformate-based Sample Preparation Protocol for Chiral and Nonchiral Amino Acid Analysis by Gas Chromatography.- The EZ:Faast Family of Amino Acid Analysis Kits: Application of the GC-FID Kit for Rapid Determination of Plasma Tryptophan and other Amino Acids.- Amino Acid Analysis in Physiological Samples by GC-MS with Propyl chloroformate Derivatization and iTRAQ-LC-MS/MS.- Automated Analysis of Primary Amino Acids in Plasma by High-Performance Liquid Chromatography.- RP-LC of Phenylthiocarbamyl Amino Acid Adducts (PTC-AAs) in Plasma Acetonitrile Extracts: Use of Multiple Internal Standards and VariableWavelength UV Detection.- Quantification of Underivatized Amino Acids on Dry Blood Spot, Plasma and Urine by HPLC-ESI-MS/MS.- Capillary Electrophoresis of Free Amino Acids in Physiological Fluids without Derivatization Employing Direct or Indirect Absorbance Detection.- Measurement of 3-nitro-tyrosine in Human Plasma and Urine by Gas Chromatography-Tandem Mass Spectrometry.- Analysis of Hydroxyproline in Collagen Hydrolysates.- Innovative and Rapid Procedure for 4-hydroxyproline Determination in Meat-based Foods.- Multiple Reaction Monitoring for the Accurate Quantification of Amino Acids: Using Hydroxyproline to Estimate Collagen Content.- Sequential Injection Chromatography for Fluorimetric Determination of Intracellular Amino Acids in Marine Microalgae.- Direct Analysis of Underivatized Amino Acids in Plant Extracts by LC-MS-MS.- Wheat Gluten Amino Acid Analysis by High-Performance Anion-Exchange Chromatography with Integrated Pulsed Amperometric Detection.- Preparative HPLC Separation of Underivatized Amino Acids for Isotopic Analysis.- Quantification of Amino Acids in a Single Cell by Microchip Electrophoresis with Chemiluminescence Detection.