Recombinant Systems in Protein Expression
International Symposium Proceedings
Published in June 1990
Book
Hardback
200 pages
978-0-444-81129-5 (ISBN)
Description
Recombinant systems are being increasingly used to express proteins for both basic research and production purposes. This volume reviews the different systems, the use of prokaryots, lower eukaryots, DNA- and retroviral vectors, expression in animal cells and transgenic systems. Emphasis is given to the expression of genes at the protein level. This book is of particular interest to researchers working in the fields of gene technology, molecular biology, experimental and industrial biology, and biochemistry.
Recombinant systems are being increasingly used to express proteins for both basic research and production purposes. This volume reviews the different systems, the use of prokaryots, lower eukaryots, DNA- and retroviral vectors, expression in animal cells and transgenic systems. Emphasis is given to the expression of genes at the protein level. This book is of particular interest to researchers working in the fields of gene technology, molecular biology, experimental and industrial biology, and biochemistry.
Recombinant systems are being increasingly used to express proteins for both basic research and production purposes. This volume reviews the different systems, the use of prokaryots, lower eukaryots, DNA- and retroviral vectors, expression in animal cells and transgenic systems. Emphasis is given to the expression of genes at the protein level. This book is of particular interest to researchers working in the fields of gene technology, molecular biology, experimental and industrial biology, and biochemistry.
More details
Language
English
Place of publication
Oxford
United Kingdom
Publishing group
Elsevier Science & Technology
Target group
College/higher education
Professional and scholarly
Dimensions
Height: 230 mm
ISBN-13
978-0-444-81129-5 (9780444811295)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Classification
Content
PROKARYOTIC SYSTEMS. The pEX vectors and their uses in molecular and cell biology (K.K. Stanley). Way to search for ligands binding to a taxon-specific lens crystallin (G.G. Gause Jr, M.V. Knyazeva, S.M. Dolgilevich and A.S. Mikaelyan). cDNA sequence and expression of cytovillin, a microvillar Mr 75 000 protein (O. Turunen, R. Pakkanen, E.-M. Salonen, T. Wahlstrom and A. Vaheri). pEX-env. A novel expression plasmid coding for a recombinant HIV-1 envelope peptide; expression and purification of the recombinant antigen for diagnostic purposes (M.F. Schinkinger, H. Mucke, W.J. Frantsits, T.F. Schulz and M.P. Dierich). LOWER EUKARYOTIC SYSTEMS. Glycosylation and export of heterologous proteins expressed in yeast (V.L. Mackay, C. Yip, S. Welch, T. Gilbert, P. Seidel, F. Grant and P. O'Hara). Yeast Expression: Secretion and intracellular systems (P.J. Barr, O.B. Mason, P.A. Wong, H.L. Gibson, M.C. Kiefer and A.J. Brake). Trichoderma reesei - A host for protein production (A. Harkki, J. Uusitalo, S. Vanhanen, M. Bailey and J. Knowles). Construction of expression vectors based on the yeast MELI gene; expression of fungal cellulase in yeast (P.L. Suominen, R. Buhler and P. Liljestrom). Secretion into the medium of hepatitis B surface antigen particles by the methylotropic yeast Hansenula polymopha (S.-H. Shen, L. Bastien and S.N. Slilaty). HIGHER EUKARYOTIC SYSTEMS. Viral vectors - DNA-viruses. The development and use of baculovirus vectors for multigene expression (D.H.L. Bishop, M. Hill-Perkins, K. Takehara, T. Urakawa, U. Weyer and R.D. Possee). Factors limiting biosynthesis and secretion of factor VIII in mammalian cells (R.J. Kaufman, D.D. Pittman, K.A. Marquette, L.C. Wasley and A.J. Dorner). An improved chloramphenicol acetyltransferase expression vector system for mapping transcriptional and post-transcriptional regulatory elements in animal cells (C.C. Baker). Expression of human papillomavirus Type 16 proteins in Escherichia coli and their use as antigens in serological tests (I. Jochmus-Kudielka and L. Gissmann). The production of adenovirus Types 5 and 12 E1A proteins in the baculovirus expression system (D.S. Peeper, A. Zantema, M.H.M. Noteborn and A.J. van der Eb). Construction of an intact equine herpesvirus Type-1 (EHV-1) glycoprotein gene and its expression in COS cells (W.A. Bonass, D.M. Elton, J.-M. Stocks, R.A. Killington, D.M. Meredith and I.W. Halliburton). Polyhedrin promotor-directed expression of rubella virus spike proteins in baculovirus-infected lepidopteran cells (C. Oker-Blom, R.F. Pettersson and M.D. Summers). Immunoglobulin double isotype expression by trans-mRNA in a human MU chain transgenic mouse (A. Shimizu, M.C. Nussenzweig, T.-R. Mizuta, P. Leder and T. Honjo). Expression in animal cells. Multiple regulatory pathways of human HSP70 gene transcription (G.T. Williams, T.K. McClanahan and R.I. Morimoto). Automatic directional cloning: An efficient method to construct eukaryotic expression cDNA libraries (T. Miki, T.