Time-Resolved Mass Spectrometry

From Concept to Applications
 
 
John Wiley & Sons Inc (Verlag)
  • erschienen am 12. Januar 2016
  • |
  • 376 Seiten
 
E-Book | PDF mit Adobe DRM | Systemvoraussetzungen
978-1-118-88734-9 (ISBN)
 
Time is an important factor in physical and natural sciences. It characterizes the progress of chemical and biochemical processes. Mass spectrometry provides the means to study molecular structures by detecting gas-phase ions with the unique mass-to-charge ratios. Time-resolved mass spectrometry (TRMS) allows one to differentiate between chemical states that can be observed sequentially at different time points. Real-time mass spectrometric monitoring enables recording data continuously with a specified temporal resolution. The TRMS approaches - introduced during the past few decades - have shown temporal resolutions ranging from hours down to microseconds and beyond.
This text covers the key aspects of TRMS. It introduces ion sources, mass analyzers, and interfaces utilized in time-resolved measurements; discusses the influence of data acquisition and treatment; finally, it reviews most prominent applications of TRMS - in the studies of reaction kinetics and mechanism, physicochemical phenomena, protein structure dynamics, biocatalysis, and metabolic profiling.
It will assist science and engineering students to gain a basic understanding of the TRMS concept, and to recognize its usefulness. In addition, it may benefit scientists who conduct molecular studies in the areas of chemistry, physics and biology.
1. Auflage
  • Englisch
  • New York
  • |
  • Großbritannien
  • Für Beruf und Forschung
978-1-118-88734-9 (9781118887349)
1118887344 (1118887344)
weitere Ausgaben werden ermittelt
  • Title Page
  • Copyright
  • Table of Contents
  • Author Biographies
  • Preface
  • Acknowledgments
  • List of Acronyms
  • Chapter 1: Introduction
  • 1.1 Time in Chemistry
  • 1.2 Mass Spectrometry
  • 1.3 Time-resolved Mass Spectrometry
  • 1.4 Dynamic Matrices
  • 1.5 Real-time vs. Single-point Measurements
  • 1.6 Further Reading
  • References
  • Chapter 2: Ion Sources for Time-resolved Mass Spectrometry
  • 2.1 Electron Ionization
  • 2.2 Chemical Ionization
  • 2.3 Atmospheric Pressure Chemical Ionization
  • 2.4 Electrospray Ionization
  • 2.5 Atmospheric Pressure Photoionization
  • 2.6 Desorption/Ionization
  • 2.7 Innovations in the 21st Century
  • 2.8 Concluding Remarks
  • References
  • Chapter 3: Mass Analyzers for Time-resolved Mass Spectrometry
  • 3.1 Overview
  • 3.2 Individual Mass Analyzers
  • 3.3 Integrated Analytical Techniques
  • References
  • Chapter 4: Interfaces for Time-resolved Mass Spectrometry
  • 4.1 Molecules in Motion
  • 4.2 Time-resolved Mass Spectrometry Systems
  • 4.3 Concluding Remarks
  • References
  • Chapter 5: Balancing Acquisition Speed and Analytical Performance of Mass Spectrometry
  • 5.1 Overview
  • 5.2 Spectrum Acquisition Speed
  • 5.3 Relationship between Spectrum Acquisition Time and Mass Spectrometer Performance
  • References
  • Chapter 6: Hyphenated Mass Spectrometric Techniques
  • 6.1 Introduction
  • 6.2 Separation Techniques Coupled with Mass Spectrometry
  • 6.3 Ion-mobility Spectrometry
  • 6.4 Other Hyphenated Systems
  • 6.5 Influence of Data Acquisition Speed
  • 6.6 Concluding Remarks
  • References
  • Chapter 7: Microfluidics for Time-resolved Mass Spectrometry
  • 7.1 Overview
  • 7.2 Fabrication
  • 7.3 Microreaction Systems
  • 7.4 Hydrodynamic Flow
  • 7.5 Coupling Microfluidics with Mass Spectrometry
  • 7.6 Examples of Applications
  • 7.7 Digital Microfluidics
  • 7.8 Concluding Remarks
  • References
  • Chapter 8: Quantitative Measurements by Mass Spectrometry
  • 8.1 The Challenge of Quantitative Mass Spectrometry Measurements
  • 8.2 Selection of Instrument
  • 8.3 Solutions to Quantitative Mass Spectrometry
  • 8.4 Data Treatment
  • 8.5 Concluding Remarks
  • References
  • Chapter 9: Data Treatment in Time-resolved Mass Spectrometry
  • 9.1 Overview
  • 9.2 Definition of Terms
  • 9.3 Spectral Patterns
  • 9.4 Mass Accuracy
  • 9.5 Structural Derivation
  • 9.6 Molecule Abundance
  • 9.7 Time-dependent Data Treatment
  • References
  • Chapter 10: Applications in Fundamental Studies of Physical Chemistry
  • 10.1 Overview
  • 10.2 Chemical Kinetics
  • 10.3 Chemical Equilibrium
  • References
  • Chapter 11: Application of Time-resolved Mass Spectrometry in the Monitoring of Chemical Reactions
  • 11.1 Organic Reactions
  • 11.2 Catalytic Reactions
  • 11.3 Photochemical Reactions
  • 11.4 Concluding Remarks
  • References
  • Chapter 12: Applications of Time-resolved Mass Spectrometry in the Studies of Protein Structure Dynamics
  • 12.1 Electrospray Ionization in Protein Studies
  • 12.2 Mass Spectrometry Strategies for Ultra-fast Mixing and Incubation
  • 12.3 Hydrogen/Deuterium Exchange
  • 12.4 Photochemical Methods
  • 12.5 Implementation of Ion-mobility Spectrometry Coupled with Mass Spectrometry
  • 12.6 Concluding Remarks
  • References
  • Chapter 13: Applications of Time-resolved Mass Spectrometry in Biochemical Analysis
  • 13.1 Enzymatic Reactions
  • 13.2 Time-resolved Mass Spectrometry in Systems and Synthetic Biology
  • 13.3 Monitoring Living Systems
  • 13.4 Concluding Remarks
  • References
  • Chapter 14: Final Remarks
  • 14.1 Current Progress
  • 14.2 Instrumentation
  • 14.3 Software
  • 14.4 Limitations
  • References
  • Index
  • End User License Agreement

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