Essential Techniques for Medical and Life Scientists: A guide to contemporary methods and current applications with the protocols: Part 1

 
 
PublishDrive
  • 1. Auflage
  • |
  • erschienen am 27. September 2018
  • |
  • 140 Seiten
 
E-Book | ePUB mit Adobe DRM | Systemvoraussetzungen
978-1-68108-709-2 (ISBN)
 

This handbook covers some primary instruments-based techniques used in modern biological science and medical research programs. Key features of the book include introductory notes for each topic, systematic presentation of relevant methods, troubleshootin

  • Englisch
  • Sharjah
  • |
  • Vereinigte Arabische Emirate
  • 1,92 MB
978-1-68108-709-2 (9781681087092)
weitere Ausgaben werden ermittelt
  • Intro
  • CONTENTS
  • FOREWORD
  • PREFACE
  • List of Contributors
  • Application of Mass Spectrometry in Proteomics
  • Serap Pektas*
  • INTRODUCTION
  • 1. INLET SYSTEMS
  • 2. IONIZATION TECHNIQUE
  • 2.1. Electrospray Ionization (ESI)
  • 2.2. Matrix Assisted Laser Desorption Ionization (MALDI)
  • 2.2.1. MALDI Sample Preparation
  • 3. MASS ANALYZERS
  • 3.1. Quadrupole Mass Analyzer
  • Tandem Mass Spectrometry MS/MS
  • 3.2. Ion Trap Mass Analyzer (IT)
  • 3.3. Orbitrap Mass Analyzer
  • 3.4. Time of Flight (TOF) Mass Analyzer
  • 4. DETECTORS
  • 5. APPLICATIONS
  • 5.1. Proteomics
  • 5.1.1. Molecular Weight Determination
  • 5.1.2. Protein Sequencing
  • 5.1.3. Post-Translational Modifications
  • 5.1.4. Protein-Protein, Protein-DNA and Protein-Small Molecule Interactions
  • 5.1.5. Protein Folding and Unfolding Rates
  • 5.2. Microbiology
  • 5.3. Medicine
  • CONSENT FOR PUBLICATION
  • CONFLICT OF INTEREST
  • ACKNOWLEDGEMENTS
  • REFERENCES
  • Structural Elucidation of Macromolecules
  • Ana Luísa Carvalho, Teresa Santos-Silva, Maria João Romão, Eurico J. Cabrita and Filipa Marcelo*
  • INTRODUCTION
  • 2.1. Solving the 3D Structure of Proteins Using X-ray Crystallography
  • 2.1.1. Protein Crystallization
  • 2.1.2. Diffraction of X-rays by Protein Crystals
  • 2.1.3. 3D Structure Solution and Refinement
  • 2.1.4. Validation and Analysis of Crystal Structures
  • 2.2. NMR Guide into Molecular Recognition
  • 2.2.1. NMR and the Study of Intermolecular Interactions
  • 2.2.2. Protein-detected NMR Based Experiments
  • 2.2.3. Ligand-detected NMR Based Experiments
  • 2.3. Studying Protein Shape and Protein-ligand Interactions by SAXS Methods
  • 2.3.1. Data Collection
  • 2.3.2. Preliminary Sample Characterization: Guinier
  • Porod, Kratky and Pair Distribution Functions
  • 2.3.3. Deriving a Structural Model
  • 2.4. Structural Elucidation Using Cryo-Electron Microscopy
  • 2.5. Notes
  • 2.5.1. Growing and Preserving a Protein Crystal
  • 2.5.2. Bragg's Law
  • 2.5.3. Seleno-methionine Derivatives for Structure Solution
  • 2.5.4. Patterson Maps and Molecular Replacement
  • 2.5.5. Electron Density Maps
  • 2.5.5. The Temperature Factor (B Factor)
  • 2.5.6. The Ramachandran Plot
  • 2.5.7. Technical Aspects of NMR Hardware and Software
  • 2.5.8. NMR Sample
  • 2.5.9. General Aspects on Acquisition and Processing of NMR Spectra
  • 2.5.10. Basics on 15N-1H-HSQC Titrations
  • 2.5.11. Basics on Transferred NOE
  • 2.5.12. Basics on STD-NMR Experiments
  • 2.5.13. Basics on Diffusion Experiments
  • 2.5.14. SAXS Buffer Subtraction
  • 2.5.15. Sample Quality for Successful BioSAXS Experiment
  • 2.5.16. Radiation Damage
  • 2.5.17. Small Angle Neutron Scattering (SANS)
  • CONSENT FOR PUBLICATION
  • CONFLICT OF INTEREST
  • ACKNOWLEDGEMENTS
  • REFERENCES
  • Isothermal Titration Calorimetry
  • Ozlem Ustun Aytekin1, Elvan Yilmaz Akyuz1, Banu Bayram1, Esen Tutar2, Halime Hanım Pence3 and Yusuf Tutar1,4,*
  • INTRODUCTION
  • 3.1. ITC in Protein Science and Enzyme Technology
  • 3.1.1. Protein-protein Interactions
  • 3.1.2. Protein- DNA/RNA Interactions
  • 3.1.3. Enzyme Kinetics
  • 3.2. ITC in Pharmaceutical Research
  • 3.2.1. ITC in Drug Delivery
  • 3.2.2. ITC in Drug Screening
  • 3.2.3. ITC in Drug Design
  • 3.2.4. ITC in Drug Development
  • 3.3. ITC Applications in Food Science
  • 3.3.1. Interactions Involving Proteins or Peptides
  • 3.3.2. Interactions Involving Carbohydrates
  • 3.3.3. Interactions Involving Lipids
  • 3.4. Protocol
  • 3.4.1. Sample Preparation
  • 3.4.2. Measurement
  • 3.4.3. Data Collection and Analysis
  • 3.5. Troubleshooting
  • CONSENT FOR PUBLICATION
  • CONFLICT OF INTEREST
  • ACKNOWLEDGEMENTS
  • REFERENCES
  • Reverse Transcription Polymerase Chain Reaction (RT-PCR)
  • Venkidasamy Baskar and Sathishkumar Ramalingam*
  • INTRODUCTION
  • 4.1. Methodology
  • 4.1.1. Isolation of RNA
  • 4.1.2. Reverse Transcription or Complementary DNA Synthesis (cDNA Synthesis)
  • 4.1.3. PCR Amplification
  • 4.2. Applications
  • 4.3. Precautions to be Taken
  • 4.3.1. Real-time Quantitative PCR (RT-qPCR)
  • 4.4. Future Perspectives
  • CONSENT FOR PUBLICATION
  • CONFLICT OF INTEREST
  • ACKNOWLEDGEMENTS
  • REFERENCES
  • SUBJECT INDEX

Dateiformat: EPUB
Kopierschutz: Adobe-DRM (Digital Rights Management)

Systemvoraussetzungen:

Computer (Windows; MacOS X; Linux): Installieren Sie bereits vor dem Download die kostenlose Software Adobe Digital Editions (siehe E-Book Hilfe).

Tablet/Smartphone (Android; iOS): Installieren Sie bereits vor dem Download die kostenlose App Adobe Digital Editions (siehe E-Book Hilfe).

E-Book-Reader: Bookeen, Kobo, Pocketbook, Sony, Tolino u.v.a.m. (nicht Kindle)

Das Dateiformat EPUB ist sehr gut für Romane und Sachbücher geeignet - also für "fließenden" Text ohne komplexes Layout. Bei E-Readern oder Smartphones passt sich der Zeilen- und Seitenumbruch automatisch den kleinen Displays an. Mit Adobe-DRM wird hier ein "harter" Kopierschutz verwendet. Wenn die notwendigen Voraussetzungen nicht vorliegen, können Sie das E-Book leider nicht öffnen. Daher müssen Sie bereits vor dem Download Ihre Lese-Hardware vorbereiten.

Bitte beachten Sie bei der Verwendung der Lese-Software Adobe Digital Editions: wir empfehlen Ihnen unbedingt nach Installation der Lese-Software diese mit Ihrer persönlichen Adobe-ID zu autorisieren!

Weitere Informationen finden Sie in unserer E-Book Hilfe.


Download (sofort verfügbar)

44,80 €
inkl. 5% MwSt.
Download / Einzel-Lizenz
ePUB mit Adobe DRM
siehe Systemvoraussetzungen
E-Book bestellen