Phenotyping Crop Plants for Physiological and Biochemical Traits

 
 
Academic Press
  • 1. Auflage
  • |
  • erschienen am 5. April 2016
  • |
  • 194 Seiten
 
E-Book | ePUB mit Adobe DRM | Systemvoraussetzungen
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978-0-12-804110-9 (ISBN)
 

Phenotyping Crop Plants for Physiological and Biochemical Traits presents a proven range of methodologies and practices for effective, efficient, and appropriate typing of crop plants. By addressing the basic principles and precautions needed when conducting crop-based experiments, this book guides the reader in selecting the appropriate method based on the growing environment, whether greenhouse, pot, field, or liquid (hydroponic). By addressing the quantification of seed traits related to growth experiments, including their viability and vigor, this book presents methodology options for optimum yield based on potential abiotic stresses.


  • Discusses various methods that can contribute to phenotyping of crop plants for various physiological and biochemical traits
  • Presents reliable techniques for phenotyping or quantifying plant characters during varied climatic conditions
  • Provides insights for selecting appropriate methodologies for specific crop growing situations
  • Identifies the most appropriate protocols and methods for analyzing crop traits


Presently he is handling one UGC project as principal investigator. He was honored with Meritorious Research Scientist award by the Acharya N G Ranga Agricultural University, A.P., India. He handled one national project and two state plan projects as Principal Investigator and 7 projects as Associate leader. He has published 85 research papers in various reputed national and international journals. He attended several national and international conferences. He has got 17 years experience in teaching and research and guided one post graduate student as chairman and 17 post graduate students as member. He is instrumental in releasing drought tolerant varieties viz., Abhaya, Greeshma, Rohini and Dharani.
  • Englisch
  • San Diego
  • |
  • USA
Elsevier Science
  • 7,89 MB
978-0-12-804110-9 (9780128041109)
0128041102 (0128041102)
weitere Ausgaben werden ermittelt
  • Cover
  • Title Page
  • Copyright Page
  • Contents
  • Message
  • Foreword
  • Preface
  • Abbreviations
  • Introduction
  • Section I
  • Chapter 1 - Various methods of conducting crop experiments
  • 1.1 - Field experiments
  • 1.2 - Experiments under green houses
  • 1.2.1 - Demerits
  • 1.3 - Experiments in growth chambers
  • 1.3.1 - Demerits
  • 1.4 - Hydroponics
  • 1.4.1 - Precautions
  • 1.5 - Pot culture
  • Section II
  • Chapter 2 - Seed physiological and biochemical traits
  • 2.1 - Destructive methods
  • 2.1.1 - Seed viability
  • 2.1.2 - Seed vigor tests
  • 2.2 - Nondestructive methods
  • 2.2.1 - X-ray analysis
  • 2.2.2 - Electrical impedance spectroscopy (EIS)
  • 2.2.3 - Multispectral Imaging
  • 2.2.4 - Microoptrode technique (MOT)
  • 2.2.5 - Infrared thermography (IRT)
  • 2.2.6 - Seed viability measurement using resazurin reagent
  • 2.2.7 - Computerized seed imaging
  • Section III
  • Chapter 3 - Plant growth measurements
  • 3.1 - Measurement of growth
  • 3.2 - Measurement of below ground biomass
  • 3.3 - Growth analysis
  • 3.3.1 - Growth characteristics-definition and mathematical formulae
  • Chapter 4 - Photosynthetic rates
  • 4.1 - Net assimilation rate (NAR)
  • 4.2 - Measuring through infrared gas analyzer (IRGA)
  • 4.3 - Rubisco enzyme activity
  • 4.3.1 - Measurement of rubisco activity
  • 4.4 - Chlorophyll fluorescence ratio (Fv/Fm values)
  • Chapter 5 - Drought tolerance traits
  • 5.1 - Water use efficiency (WUE) traits
  • 5.1.1 - Carbon isotope discrimination
  • 5.1.2 - Determination of stable carbon isotopes using isotope ratio mass spectrometer (IRMS)
  • 5.1.3 - Protocol for carbon isotope discrimination in leaf biomass
  • 5.2 - Root traits
  • Chapter 6 - Other drought-tolerant traits
  • 6.1 - Relative water content (RWC)
  • 6.2 - Chlorophyll stability index (CSI)
  • 6.3 - Specific leaf nitrogen (SLN)
  • 6.4 - Mineral ash content
  • 6.5 - Leaf anatomy
  • 6.6 - Leaf pubescence density
  • 6.7 - Delayed senescence or stay-greenness
  • 6.8 - Leaf waxiness
  • 6.9 - Leaf rolling
  • 6.10 - Leaf thickness (mm)
  • 6.11 - Stomatal index and frequency
  • 6.12 - Other indicators for drought tolerance
  • 6.13 - Phenological traits
  • Chapter 7 - Tissue water related traits
  • 7.1 - Osmotic potential
  • 7.1.1 - Determination of osmotic potential using vapor pressure osmometer
  • 7.1.1.1 - Procedure
  • 7.2 - Leaf water potential
  • 7.3 - Relative water content
  • 7.4 - Cell membrane injury
  • 7.4.1 - Cell membrane permeability based on leakage of solutes from leaf samples
  • Chapter 8 - Heat stress tolerance traits
  • 8.1 - Canopy temperature
  • 8.2 - Chlorophyll stability index (CSI)
  • 8.3 - Chlorophyll fluorescence
  • 8.4 - Thermo induction response (TIR) technique
  • 8.5 - Membrane stability index
  • 8.5.1 - Membrane permeability based on leakage of solutes from leaf samples
  • Chapter 9 - Oxidative stress tolerance traits
  • 9.1 - Oxidative damage
  • 9.1.1 - Antioxidant enzymes
  • 9.2 - Superoxide dismutase (SOD)
  • 9.3 - Catalase
  • 9.4 - Peroxidase (POD)
  • 9.5 - Free radicals
  • Chapter 10 - Salinity tolerance traits
  • 10.1 - Chlorophyll stability index
  • 10.2 - Proline
  • 10.3 - Sodium (Na) and potassium (K) ratio
  • 10.3.1 - Potassium (K)
  • 10.3.2 - Sodium (Na)
  • 10.4 - Antioxidative enzymes
  • Section IV
  • Chapter 11 - Kernel quality traits
  • 11.1 - Proteins
  • 11.1.1 - Protein Estimation by Lowry Method
  • 11.1.2 - Protein estimation by Bradford method
  • 11.2 - Kernel oil
  • 11.2.1 - Oil estimation by soxhlet apparatus (SOCS)
  • 11.3 - Aflatoxins
  • 11.3.1 - Quantification of aflatoxin levels in kernels
  • Chapter 12 - Carbohydrates and related enzymes
  • 12.1 - Reducing sugars
  • 12.2 - Nonreducing sugars
  • 12.3 - Total carbohydrates
  • 12.4 - Estimation of sucrose phosphate synthase
  • 12.5 - Estimation of starch synthase
  • 12.6 - Estimation of invertases
  • Chapter 13 - Nitrogen compounds and related enzymes
  • 13.1 - Total nitrogen
  • 13.1.1 - Kjeldhal method for quantifying leaf nitrogen content
  • 13.1.2 - Preparation of reagents
  • 13.1.3 - Protein percent can be determined indirectly using the following formula
  • 13.2 - Total free amino acids
  • 13.3 - Nitrate reductase
  • 13.4 - Nitrite reductase
  • 13.5 - Leghemoglobin (Lb)
  • 13.6 - Glutamic acid dehydrogenase (GDH)
  • 13.7 - Glutamate synthase (GOGAT)
  • 13.8 - Glutamine synthetase (GS)
  • 13.8.1 - Calculation
  • Chapter 14 - Other biochemical traits
  • 14.1 - Total phenols
  • 14.2 - Ascorbic acid
  • 14.3 - Alcohol dehydrogenase (ADH)
  • 14.4 - Glycine betaine
  • Chapter 15 - Plant pigments
  • 15.1 - Chlorophylls
  • 15.1.1 - Estimation of chlorophyll
  • 15.2 - Carotenoids
  • 15.2.1 - Quantification of carotenoids in green leaves
  • 15.3 - Lycopene
  • 15.4 - Anthocyanin
  • Chapter 16 - Growth regulators
  • 16.1 - Estimation of indole acetic acid (IAA)
  • 16.2 - Estimation of gibberellins
  • 16.3 - Estimation of abscisic acid (ABA)
  • 16.4 - Estimation of ethylene
  • Section V
  • Chapter 17 - Analytical techniques
  • 17.1 - Ultraviolet visible (UV-VIS) spectrophotometer
  • 17.2 - Thin layer chromatography (TLC)
  • 17.3 - Gas chromatography (GC)
  • 17.3.1 - Introduction
  • 17.3.2 - Principle
  • 17.3.3 - Detectors
  • 17.3.3.1 - Thermal conductivity detector
  • 17.3.3.2 - Flame ionization detector
  • 17.3.3.3 - Electron capture detector
  • 17.4 - High-performance liquid chromatography (HPLC)
  • 17.4.1 - Role of five major HPLC components
  • 17.5 - Liquid chromatography-mass spectrometry (LC-MS, or alternatively HPLC-MS)
  • 17.5.1 - Flow splitting
  • 17.5.2 - Mass spectrometry (MS)
  • 17.5.3 - Mass analyzer
  • 17.5.4 - Interface
  • 17.5.5 - Applications
  • 17.5.5.1 - Pharmacokinetics
  • 17.5.5.2 - Proteomics/metabolomics
  • 17.6 - Inductively coupled plasma spectrometry (ICP) (Soil & Plant Analysis Laboratory University of Wisconsin-Madison htt...
  • 17.6.1 - Introduction
  • 17.6.2 - Summary of method
  • 17.6.3 - Safety
  • 17.6.4 - Interference
  • 17.6.5 - Measurement by ICP-OES
  • 17.6.5.1 - Sample preparation
  • 17.6.6 - Measurement
  • 17.6.7 - Measurement by ICP-MS
  • 17.6.7.1 - Sample preparation
  • 17.6.8 - Measurement
  • Appendices - Common Buffers
  • Appendix I: citrate buffer
  • Appendix II: sodium phosphate buffer
  • Appendix III: potassium phosphate buffer
  • Appendix IV: sodium acetate buffer
  • Appendix V: Tris-HCl buffer (tris-hydroxymethyl aminomethane hydrochloric acid buffer)
  • Appendix VI: 1M Hepes-NaOH pH 7.5 buffer
  • Appendix VII: preparation of stocks of macro and micronutrients for hydroponics experiment
  • Appendix VIII: preparation of 'Hoagland solution' for hydroponics experiment
  • Appendix IX: solubility chart of plant growth regulators
  • References
  • Index
  • Back Cover

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