Biotechnology in Flavor Production

Wiley-Blackwell (Verlag)
  • 2. Auflage
  • |
  • erschienen am 2. August 2016
  • |
  • 336 Seiten
E-Book | ePUB mit Adobe DRM | Systemvoraussetzungen
978-1-118-35403-2 (ISBN)
Throughout history, human beings have sought ways to enhance the flavor of the foods they eat. In the 21st century, biotechnology plays an important role in the flavor improvement of many types of foods. This book covers many of the biotechnological approaches currently being applied to flavor enhancement. The contribution of microbial metabolism to flavor development in fermented beverages and dairy products has been exploited for thousands of years, but the recent availability of whole genome sequences of the yeasts and bacteria involved in these processes is stimulating targeted approaches to flavor enhancement.
Chapters discuss recent developments in the flavor modification of wine, beer, and dairy products through the manipulation of the microbial species involved. Biotechnological approaches to the production of specific flavor molecules in microbes and plant tissue cultures, and the challenges that have been encountered, are also covered, along with the metabolic engineering of food crops for flavor enhancement - also a current area of research. Biotechnology is also being applied to crop breeding through marker-assisted selection for important traits, including flavor, and the book looks at the application of the biotechnological approach to breeding for enhanced flavor in rice, apple, and basil. These techniques are subject to governmental regulation, and this is addressed in a dedicated chapter.
This updated second edition features five brand new chapters, and the topics covered in the book will be of interest to those in the flavor and food industries as well as to academic researchers interested in flavors.
2. Auflage
  • Englisch
  • New York
  • |
  • Großbritannien
John Wiley & Sons
  • 7,17 MB
978-1-118-35403-2 (9781118354032)
1118354036 (1118354036)
weitere Ausgaben werden ermittelt
  • Cover
  • Title Page
  • Copyright
  • Contents
  • Contributors
  • Preface
  • Chapter 1 The flavor of citrus fruit
  • Introduction
  • Taste components of citrus fruit
  • Sugars
  • Acids
  • Bitter compounds
  • Aroma compounds of citrus fruit
  • Terpene hydrocarbons
  • Aldehydes
  • Alcohols
  • Esters
  • Ketones
  • Other volatiles
  • Citrus genes involved in flavor production
  • The unique flavor of different citrus species
  • The flavor of oranges
  • The flavor of mandarins
  • The flavor of grapefruit
  • The flavor of lemons
  • Accumulation of off-flavors in fresh citrus fruit during postharvest storage
  • Flavor of citrus essential oils
  • Acknowledgments
  • References
  • Chapter 2 Aroma as a factor in the breeding process of fresh herbs-the case of basil
  • The importance of selecting for aroma in breeding of aromatic plants
  • The importance of genetic factors regarding the essential oil composition in aromatic plants
  • Sweet basil and the Ocimum genus
  • Uses of sweet basil
  • The chemistry of the aroma factors of plants: the essential oil
  • Essential oil profiles of common commercial basil varieties
  • Comparison of chemical analysis methods
  • Variation of the volatile compound composition within the plant
  • Variation of aroma compounds within cultivars and the potential for selection
  • Biosynthetic pathways of basil aroma components
  • Inheritance of aroma compounds in basil
  • Interspecific hybridization among Ocimum species
  • Applications of biotechnology-based approaches to modification of basil aroma
  • References
  • Chapter 3 Novel yeast strains as tools for adjusting the flavor of fermented beverages to market specifications
  • Introduction
  • Wine
  • Beer
  • Saké
  • Wine, beer, and saké yeasts
  • Wine yeasts
  • Beer yeasts
  • Saké yeasts
  • Acids
  • Non-volatile acids
  • Volatile acids
  • Alcohols
  • Ethanol
  • Glycerol
  • Higher alcohols
  • Esters
  • Carbonyl compounds
  • Acetaldehyde
  • Diacetyl
  • Volatile phenols
  • Sulfur compounds
  • Sulfides
  • Mercaptans
  • Thiols
  • Monoterpenoids
  • Conclusion
  • References
  • Chapter 4 Biotechnology of flavor formation in fermented dairy products
  • Introduction
  • Biochemistry of dairy fermentations
  • Biotechnology and flavor
  • Flavor production from bacteria
  • Comparative genomics of flavor production
  • Expression and metabolite analysis
  • Predictive bioinformatics
  • Non-culturable lactococci
  • Translation of omics to biotechnology
  • Conclusion
  • References
  • Chapter 5 Biotechnological production of vanillin
  • Introduction
  • Biosynthesis of vanillin
  • Natural occurrence of vanillin
  • Site of vanillin production in vanilla beans
  • Vanillin biosynthetic pathway in Vanilla planifolia
  • Production of vanillin by biotechnology
  • Introduction
  • Use of microorganisms
  • Use of plant tissue culture
  • Use of enzymes
  • Use of physical and mild chemistry methods
  • Synthetic vanillin
  • Vanillin from vanilla beans
  • Regulations
  • Conclusions and future outlook
  • References
  • Chapter 6 Plant cell culture as a source of valuable chemicals
  • Introduction
  • Establishment of callus culture
  • Initiation and maintenance of cell culture
  • Production of valuable chemicals by cultured plant cells
  • Metabolic engineering to improve chemical production
  • Concluding remarks
  • References
  • Chapter 7 Increasing the methional content in potato through biotechnology
  • Flavor compound methional in foods
  • Formation of methional
  • Synthesis of Met in plants
  • Biotechnology to enhance Met and methional
  • References
  • Chapter 8 Flavor development in rice
  • Introduction
  • Old flavors of rice
  • Rice texture
  • Fragrant rice
  • The chemistry of rice fragrance
  • The genetics of rice fragrance
  • BAD enzymes and 2AP synthesis
  • The future
  • References
  • Chapter 9 Tomato aroma: biochemistry and biotechnology
  • The major aroma impact volatiles in tomato and their biosynthetic pathways
  • Biosynthesis of tomato volatiles
  • Degradation of fatty acids
  • Volatiles derived from amino acids
  • Terpenes
  • Carotenoid pigmentation affects the flavor and volatile composition of tomato fruit
  • Genetic engineering of tomato aroma
  • Contribution of "omics" to improving our understanding of aroma biosynthesis and perception
  • Conclusion
  • Acknowledgment
  • References
  • Chapter 10 Breeding and biotechnology for flavor development in apple (Malus × domestica Borkh.)*
  • Quality
  • Apple volatiles
  • Ester compounds and ester biosynthesis
  • Measurement techniques
  • Varietal and developmental differences
  • Effect of storage
  • Effect of processing
  • Effect of 1-methylcyclopropene treatment
  • Hypoxia
  • Gene isolation
  • Genetic studies, linkage maps, and marker-assisted selection
  • ESTs
  • Transgenic approaches
  • Ethylene production and softening (ACS-ACO)
  • Consumer perceptions and sensory testing
  • References
  • Chapter 11 Biosynthesis and perception of melon aroma
  • Introduction
  • Volatile composition of melon fruit
  • Odor perception
  • Biosynthesis of melon aroma volatiles
  • Terpenoids
  • Fatty acid-derived volatile aldehydes
  • Amino acid-derived aroma compounds
  • Formation of volatile alcohols from volatile aldehydes
  • Formation of volatile esters from volatile alcohols
  • The interphase between volatile and non-volatile metabolites
  • Changes of volatile profiles in transgenic melons inhibited in ethylene production
  • Concluding remarks
  • References
  • Index
  • Supplemental Images
  • EULA

Chapter 1
The flavor of citrus fruit

Ron Porat, Sophie Deterre, Pierre Giampaoli and Anne Plotto


Citrus is the most important cultivated fruit tree crop in terms of area and production values. It is grown commercially in more than 140 countries in tropical and subtropical regions of the world, with total annual production of over 100 million tons and providing a contribution of US$6-8 billion to the world economy (Ladaniya 2008; USDA 2012).

The genus Citrus belongs to the Rutaceae family, subfamily Aurantioidae, and originates from Southeast Asia, nearby North India, Myanmar, and China (Swingle and Reece 1967; Scora 1975). According to the classification by Swingle, the most commercially important citrus species are sweet orange (C. sinensis), sour orange (C. aurantium), mandarin (C. reticulata), grapefruit (C. paradisi), pummelo (C. grandis), lemon (C. limon), citron (C. medica), and lime (C. aurantifolia). Furthermore, phylogenetic and taxonomic studies of the genus Citrus revealed that there are only three basic "true" citrus ancestors, which are citron (C. medica), mandarin (C. reticulata) and pummelo (C. grandis), and all other Citrus species were actually evolved from crosses between these true original citrus species or other relatives (Scora 1975; Barrett and Rhodes 1976). For example, sweet orange was derived from a cross between mandarin and pummelo; grapefruit was derived from a cross between pummelo and sweet orange; and lemon was derived from a cross between citron and sour orange (Barkley et al. 2006; Li et al. 2010).

From a botanical perspective, citrus fruit is a hesperidium, i.e., a special type of berry with a leathery rind internally divided into segments (Grierson 2006a). The fruit is anatomically divided into three separate layers: the outer colored portion of the rind called the flavedo or exocarp, which includes the cuticle, the colored epidermis cells containing chlorophyll or carotenoid pigments and the hypodermis cells consisting of the oil glands; the inner white portion of the peel called the albedo or mesocarp, which is comprised of spongy parenchymous cells; and the internal part of the fruit called flesh or endocarp, which represents the edible portion of the fruit including the juice sacs, segment membranes, and seeds (Schneider 1968; Grierson 2006a) (Fig. 1.1). From the nutritional aspect, citrus fruit provide an important beneficial source to the human diet for consumption of ascorbic acid (vitamin C) and folic acid (vitamin B9), pectin and soluble fibers, different minerals, carotenoids, and specific flavonoids and limonoids, all phytonutrients playing a role in preventing degenerative diseases such as heart diseases and various types of cancers (Patil et al. 2006).

Fig. 1.1 Morphological structure of citrus fruit. (a) Cross-sectional view of an orange fruit. (b) Cross-section of the flavedo layer with oil glands beneath; magnification ×8. Source: Ron Porat.

Citrus fruit are either grown for fresh consumption or for juice and/or peel oil manufacturing. With respect to fresh consumption, the main producing countries are China, Brazil, Spain, Mexico and the United States, while with respect to juice manufacturing, the main producing countries are Brazil and the United States (Florida), with sweet oranges being the main product followed by grapefruit and lemons (Ladaniya 2008; USDA 2012). It is worth noting that during the last few years, consumption of fresh oranges, grapefruit, lemons and limes remained constant, whereas easy-to-peel mandarins and tangerines have seen a steady and significant increase (Ladaniya 2008; USDA 2012).

Above all, citrus fruit are appreciated and consumed by billions of people around the globe because of their unique delicate and attractive flavor evolved from a blend of fruity and freshness and earthy notes. In fact, what we perceive as flavor of citrus fruit is actually the combination of basic taste, aroma, and mouth-feel sensations that are perceived simultaneously by the brain during the eating of foods (Goff and Klee 2006). The sensation of taste providing sweet, sour, bitter, salty, and umami attributes is perceived by receptors present on the tongue and in the mouth that bind soluble components in the food matrix, whereas sensation of aroma is perceived via receptors present in the olfactory bulb in the nose cavity that specifically bind thousands of different volatiles providing various kinds of floral, fruity, minty, woody, mushroom, and other odors (Schwab et al. 2008). In this chapter, we discuss the sensory quality and biochemical constituents involved in creating the unique flavor of different citrus fruit species, including oranges, mandarins, grapefruit, and lemons. The chapter focuses on describing the flavor attributes of fresh citrus fruit and essential oils, but not of processed juices. For further information regarding the effects of juice manufacturing processes, such as extraction methods, pulp separation, thermal processing, and concentration and reconstitution methods on orange volatiles, readers are referred to the excellent review by Perez-Cacho and Rouseff (2008b).

Taste components of citrus fruit

The taste of citrus fruit is principally governed by the levels of sugars and acids in the juice sacs and the relative ratio among them; the latter relationship is also termed the total soluble solids to titratable acidity ratio (TSS : TA), or fruit ripening ratio, and is widely used by growers as an indicator of fruit maturity. During fruit ripening, juice TSS levels gradually increase whereas acidity levels gradually decrease, resulting in a continuous rise in the relative ripening ratio of the fruit (Ramana et al. 1981; Grierson 2006b). For example, the ripening ratios of navel oranges in California increase from a low level of 6 in September to above 20 in January (Obenland et al. 2009), and the ripening ratios of "Or" mandarins in Israel increase from 9 in January to 18 in March (R. Porat, unpublished data).

Because of these dynamic changes in TSS and TA levels during citrus fruit maturation (continuous increase in TSS and decrease in acidity), the overall taste of the fruit will vary with the ripening stage; within each cultivar, early-season fruit are more sour than late-season fruit. Therefore, to make sure that the fruit will not be harvested too early when they may be too sour for the market, maturity and grade standards were developed in each country and enforced by local plant protection and inspection services (Grierson 2006b). For example, in Florida, it is permitted to harvest tangerines only when their TSS levels are above 9% and TSS : TA is greater than 7.5, whereas in Israel, export of early-season Satsuma mandarins is allowed only when TSS levels are above 12% and juice acidity levels are below 1.3%, resulting in TSS : TA greater than 7.0 (Tietel et al. 2010a). In California, the minimum allowed TSS : TA for harvesting and marketing of Navel oranges is 8, even though it was shown that consumer acceptability was higher at a ripening ratio of 10 (Obenland et al. 2009). Obviously, harvesting non-mature sour fruit is not recommended because it might deter consumers from buying more fruit later in the season. However, it is also not recommended to harvest over-mature fruit, which will have a too high ripening ratio, since those fruit will suffer from low flavor preference scores (Grierson 1995). Therefore, each citrus species should be harvested at its optimal and preferred maturity index (between 8 and 12 for oranges), and either too high or too low ripening ratios are not desirable. Furthermore, it was proposed that a good tasty fruit should have high levels of sugars and moderate levels of acids rather than any other combination which may result in a similar ripening ratio (Kader 2008).

In addition to the conventional measurements of TSS : TA ratios to monitor the degree of fruit maturation, Jordan et al. (2001) suggested a new formula to evaluate the sweetness to sourness ratios termed BrimA, which takes into account the fact that receptors on the tongue have a different response to sugars and acids, and that small changes in acids are much more easily perceived than small changes in sugars. The BrimA index is derived by subtracting a multiple of TA from TSS, so that BrimA = TSS - k(TA), with constant k being characteristic of a fruit product. In the case of Navel oranges, a better correlation was found between flavor hedonic scores and sugar and acid concentrations using the BrimA index (with k = 3) rather than using the standard TSS : TA ratio, and that was true especially for low acid-containing fruit (Obenland et al. 2009). A better correlation between sweetness intensity determined by a trained panel and BrimA (r2 = 0.92) as compared with using the TSS : TA ratio (r2 = 0.76) or TSS alone (r2 = 0.74) was also found by Plotto and co-workers (unpublished data).

In the following sections, we describe the biochemical components involved in creating the sweet, sour, and bitter tastes in citrus fruit.


In most citrus species (apart from lemons that contain high amounts of acids and low amounts of sugars), sugars provide about 80% of the juice TSS content, and therefore TSS measurements provide a useful and simple indicator to evaluate total sugar levels (Erickson 1968). Table 1.1 provides data regarding...

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