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Teratogenicity Testing

Methods and Protocols
Luís Félix(Herausgeber*in)
Humana (Verlag)
2. Auflage
Erschienen am 29. Januar 2024
XVI, 627 Seiten
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978-1-0716-3625-1 (ISBN)
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This fully updated volume collects laboratory techniques and tests to assess the risks to embryo-fetal development from drug exposure during early developmental stages. Beginning with a section detailing basic principles of teratogenicity, the book continues with application of in vitro models for teratogenic screening to predict toxicity in embryonic animals and humans, as well as a variety of well-established in vivo animal model tests, which offer solutions for evaluating the efficacy and the teratogenicity of compounds in preclinical trials before human safety testing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Teratogenicity Testing: Methods and Protocols, Second Edition serves as an ideal guide to tests that can be performed in cells, organs, tissues, and animal models for evaluating toxicity and/or safety of compounds in early developmental stages with the goal of estimating, preventing, or minimizing the teratogenic potential of drugs.

Part I: Introduction to Teratology Testing

1. Updating an Overview of Teratology

Ana Margarida Calado, Fernanda Seixas, and Maria dos Anjos Pires

2. Guidelines on Development Toxicity Tests: Brief Insights

Sofia Alves-Pimenta, Luís Félix, Bruno Colaço, Paula A. Oliveira, and Carlos Venâncio

3. Development Features on the Selection of Animal Models for Teratogenic Testing

Sofia Alves-Pimenta, Bruno Colaço, Paula A. Oliveira, and Carlos Venâncio

4. Virus as Teratogenic Agents

Ana Margarida Calado, Fernanda Seixas, and Maria dos Anjos Pires

5. The Future of the Teratogenicity Testing

Muhsin Aydin, Banu Guven Ezer, and Eyyup Rencuzogullari

6. The First Steps on AOPs' Concepts, Development, and Applications in Teratology

Leonardo Vieira, Terezinha Souza, and Davi Farias

7. Machine Learning to Predict Teratogenicity: Theory and Practice

Latifa Douali

Part II: Experimental-Based Teratology Testing

8. Human Pluripotent Stem Cell-Based Assays to Predict Developmental Toxicity

Madhura Banerjee, Aritrika Das, Prarthana Chatterjee, and Satarupa Banerjee

9. Developmental Toxicity Using the Rat Whole Embryo Culture

Zeliha Fazliogullari

10. A Modified Murine Embryonic Stem Cell Test for Evaluating the Teratogenic Effects of Drugs

Mahshid Hodjat and Mohammad Abdollahi

11. Teratogenic Effects of Drugs on Primary Lymphocytes Assessed by Flow Cytometry

Ahmad Salimi and Zhaleh Jamali

12. Assessment of the Teratogenic Effect of Drugs on the Chicken Embryo

Lucas Rosa Fraga, Maikel Rosa de Oliveira, Karina Maria Wermann, and Neil Vargesson

13. Daphnia magna as a Model Organism to Predict the Teratogenic Effect of Different Compounds

Monalisa Mishra

14. Caenorhabditis elegans as an In Vivo Model Organism to Elucidate Teratogenic Effects

Zhenglu Wang

15. Enduring Ethanol-Induced Behavioral Alterations in Caenorhabditis elegans after Developmental Lead Exposure

Lucia E. Fernandez-Hubeid, Paula A. Albrecht, Michael Aschner, and Miriam B. Virgolini

16. Exploration of Teratogenic and Genotoxic Effects on Model Organism Drosophila melanogaster

Seekha Naik and Monalisa Mishra

17. Ecotoxicological Assessment of Seaweed-Based Crop Biostimulant on Earthworm Eudrilus eugeniae Kinb

Kanagaraj Muthu-Pandian Chanthini and Sengottayan Senthil-Nathan

18. Evaluation of the Ecotoxicology of Seaweed-Based Biopesticide Used in Combat of the Polyphagous Pest Using Eudrilus eugeniae Kinb

Kanagaraj Muthu-Pandian Chanthini, Haridoss Sivanesh, Ramakrishnan Ramasubramanian, Vethamonickam Stanley-Raja, Sengodan Karthi, Narayanan Shyam-Sundar, and Sengottayan Senthil-Nathan

19. Biochemical Studies to Understand Teratogenicity and Lethality Outcomes in Modified-FETAX

Abbas Güngördü and Duygu Ozhan Turhan

20. Bioinformatics Methods for Transcriptome Analysis on Teratogenesis Testing

Thayne Woycinck Kowalski, Giovanna Câmara Giudicelli, Julia do Amaral Gomes, Mariana Recamonde-Mendoza, and Fernanda Sales Luiz Vianna

21. Angiogenesis Assay for Live and Fixed Zebrafish Larvae

Vinoth S, Satheeswaran Balasubramanian, Ekambaram Perumal, and Kirankumar Santhakumar

22. High-Resolution Respirometry for the Assessment of Teratogenic Chemicals

Christopher L. Souders II, Isaac Konig, and Christopher J. Martyniuk

23. A Stereological Approach to Quantify Immunohistochemical Staining in Zebrafish Larvae

Dércia Santos, Sandra M. Monteiro, and Ana Luzio

24. Whole-Mount Immunohistochemical and Immunofluorescence Assays in Zebrafish Embryos

Mine Köktürk and Fikret Altındag

25. Assessment of Developmental Neurotoxicity Using Semi-Automatic Behavior Analysis System for Zebrafish

Derya Cansız, Ismail Ünal, Merih Beler, A. Ata Alturfan, and Ebru Emekli-Alturfan

26. Behavioral Profiling of Zebrafish (Danio rerio) Larvae: Activity, Anxiety, Avoidance, and Startle Response

Ana M. Valentim

27. Alcian Blue Staining for Chondrocranium Development in Zebrafish

Merih Beler, Ismail Ünal, Derya Cansız, and Ebru Emekli-Alturfan

28. Protocol of Geometric Morphometrics for Teratogenicity Testing

Mariana Caipira Lei, Luís Félix, and Carlos Venâncio

29. Biochemical Markers for Liver Injury in Zebrafish Larvae

Rafael Xavier Martins, Juliana Alves Costa Ribeiro Souza, Maria Eduarda Maia, Matheus Carvalho, Terezinha Souza, and Davi Farias

30. Cortisol Quantification for Assessing Stress-Induced Changes in Zebrafish Larvae

Raquel S.F. Vieira, Carlos Venâncio, and Luís Félix

31. Metabolomic Fingerprint Assay in Zebrafish Embryos

Theodoros G. Chatzimitakos, Ioannis Leonardos, and Constantine D. Stalikas

32. Alkaline Comet Assay to Assess Genotoxicity in Zebrafish Larvae

Ondina Ribeiro, Isabel Gaivão, and João Soares Carrola

33. A Chromogenic Quantification of Protein Expression in Zebrafish Larvae

Luís Félix

34. Confocal Microscopy Technique in Teratogenicity Testing Using Zebrafish (Danio rerio) Embryos as Model

Thangal Yumnamcha, Reema Khangembam, Sangeeta Yanglem, Kshetrimayum Ophelia, Sonali Thokchom, and Damayanti Maibam

35. Whole-Mount RNA In Situ Hybridization of Zebrafish Embryos

Ismail Ünal, Derya Cansız, Merih Beler, A. Ata Alturfan, and Ebru Emekli-Alturfan

36. Quantitative Real-Time PCR Method to Evaluate Gene Expression in Zebrafish Embryos

Merih Beler, Derya Cansız, Ismail Ünal, and Ebru Emekli-Alturfan

37. Microarray Analysis to Determine Gene Expression Changes in Zebrafish Embryos

Ismail Ünal, Merih Beler, Derya Cansız, and Ebru Emekli-Alturfan

38. Cell-Free DNA and Next-Generation Sequencing for Prenatal Diagnosis

Klaus Rieneck

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