Zebrafish: Cellular and Developmental Biology, Part A Cellular Biology

Zebrafish: Cellular and Developmental Biology, Part A Cellular Biology
 
 
Academic Press
  • 4. Auflage
  • |
  • erschienen am 4. Juni 2016
  • |
  • 322 Seiten
 
E-Book | ePUB mit Adobe DRM | Systemvoraussetzungen
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978-0-12-803489-7 (ISBN)
 

The Zebrafish: Cellular and Developmental Biology, Part A Cellular Biology, is the latest edition in the Methods in Cell Biology series that looks at methods for analyzing cellular and developmental biology of zebrafish. Chapters cover such topics as cell biology and developmental and neural biology.


  • Covers sections on model systems and functional studies, imaging-based approaches, and emerging studies
  • Written by experts in the field
  • Contains cutting-edge material on the topic of developmental biology in zebrafish
  • New two part edition of this important volume
0091-679X
  • Englisch
  • San Diego
  • |
  • USA
Elsevier Science
  • 16,70 MB
978-0-12-803489-7 (9780128034897)
0128034890 (0128034890)
weitere Ausgaben werden ermittelt
  • Front Cover
  • Methods in Cell Biology
  • Series Editors
  • Methods in Cell Biology: The Zebrafish: Cellular and Developmental Biology, Part A Cellular Biology
  • Copyright
  • Dedication
  • Contents
  • Contributors
  • Preface
  • 1 - Embryonic cell culture in zebrafish
  • 2 - Cellular dissection of zebrafish hematopoiesis
  • 3 - Second harmonic generation microscopy in zebrafish
  • 4 - Imaging blood vessels and lymphatic vessels in the zebrafish
  • 5 - An eye on light-sheet microscopy
  • 6 - Single neuron morphology in vivo with confined primed conversion
  • 7 - Visualizing retinoic acid morphogen gradients
  • 8 - Using fluorescent lipids in live zebrafish larvae: from imaging whole animal physiology to subcellular lipid tr ...
  • 9 - Analysis of cilia structure and function in zebrafish
  • 10 - Functional calcium imaging in zebrafish lateral-line hair cells
  • 11 - Physiological recordings from the zebrafish lateral line
  • Volumes in Series
  • Index
  • D
  • E
  • G
  • K
  • M
  • O
  • Q
  • T
  • U
  • Z
  • Back Cover
  • 3. RECORDING MICROPHONIC POTENTIALS
  • 4. IN VIVO HAIR CELL PHYSIOLOGY
  • 5. AFFERENT NEURON ACTION CURRENTS
  • 2. STIMULATION OF NEUROMAST HAIR CELLS
  • 6. SUMMARY
  • Acknowledgments
  • REFERENCES
  • INTRODUCTION
  • 1. COMMON METHODS FOR LATERAL LINE ELECTROPHYSIOLOGY
  • 4. FUTURE DIRECTIONS
  • Acknowledgments
  • REFERENCES
  • SUMMARY
  • Acknowledgments
  • REFERENCES
  • 2. IMAGING SYSTEMS AND OPTIMAL PARAMETERS
  • 3. IMAGE PROCESSING
  • INTRODUCTION
  • 1. CALCIUM INDICATOR SELECTION AND COMPARISON
  • SUMMARY
  • Acknowledgments
  • REFERENCES
  • 3. PHENOTYPES OF CILIA MUTANTS IN ZEBRAFISH
  • INTRODUCTION
  • 1. CILIA IN ZEBRAFISH ORGANS
  • 2. VISUALIZING LIPID METABOLISM USING BODIPY FATTY ACID ANALOGS
  • 2. ANALYTICAL TOOLS FOR CILIA MORPHOLOGY AND MOTILITY
  • INTRODUCTION
  • THE NEED FOR WHOLE ANIMAL STUDIES OF LIPID METABOLISM
  • 1. FORWARD GENETIC SCREENING WITH FLUORESCENT LIPIDS
  • CONCLUSION
  • CONCLUSION AND OUTLOOK
  • REFERENCES
  • INTRODUCTION
  • 1. CHALLENGES FOR MORPHOGEN GRADIENT STUDIES
  • 2. FEEDBACK ALLOWS RETINOIC ACID TO ACT AS A GRADED MORPHOGEN
  • 3. CYP26S AS KEY REGULATORS OF RETINOIC ACID GRADIENT FORMATION
  • 4. VISUALIZING THE RETINOIC ACID GRADIENT
  • 5. CRABPS AND RETINOIC ACID SIGNAL ROBUSTNESS
  • 6. SHARPENING BOUNDARIES OF GENE EXPRESSION IN RESPONSE TO RETINOIC ACID GRADIENTS
  • 7. NOISE-BOTH GOOD AND BAD
  • 8. OTHER BOUNDARIES AND OTHER MORPHOGENS
  • CONCLUSIONS AND PERSPECTIVES
  • Acknowledgments
  • REFERENCES
  • INTRODUCTION
  • 1. PHOTOCONVERTIBLE FLUORESCENT PROTEINS
  • 2. CONFINED PRIMED CONVERSION
  • 3. UNRAVELING SINGLE NEURON MORPHOLOGY WITH CONFINED PRIMED CONVERSION
  • 3. DATA ACQUISITION AND HANDLING
  • 4. CHALLENGES AND PERSPECTIVES
  • Acknowledgment
  • REFERENCES
  • CONCLUSION
  • REFERENCES
  • 2. THE MICROSCOPE FOR YOUR SAMPLE OR THE SAMPLE FOR YOUR MICROSCOPE?
  • INTRODUCTION
  • 1. PRINCIPLE BEHIND SELECTIVE PLANE ILLUMINATION MICROSCOPY
  • 3. VITAL IMAGING OF BLOOD AND LYMPHATIC VESSELS
  • INTRODUCTION
  • 1. IMAGING VASCULAR GENE EXPRESSION
  • 2. NONVITAL BLOOD VESSEL AND LYMPHATIC VESSEL IMAGING
  • 3. NOTES
  • SUPPLEMENTARY DATA
  • REFERENCES
  • INTRODUCTION
  • 1. MATERIALS
  • 2. METHODS
  • 3. ENRICHMENT OF HSCS
  • 4. IN VITRO CULTURE AND DIFFERENTIATION OF HEMATOPOIETIC PROGENITORS
  • CONCLUSIONS
  • REFERENCES
  • INTRODUCTION
  • 2. HEMATOPOIETIC CELL TRANSPLANTATION
  • INTRODUCTION
  • 1. METHODS
  • 1.1 Blastomere Cell Culture
  • 2.1 Embryonic Donor Cells
  • 2.2 Adult Donor Cells
  • 1.1 Primitive Hematopoiesis
  • 1.2 Definitive Hematopoiesis
  • 1.3 Adult Hematopoiesis
  • 1.2 Neural Crest Cell Culture
  • 4.2 Clonal Methylcellulose-Based Assays
  • 4.1 Stromal Cell Culture Assays
  • 2.1 Macrophage-Specific Protein Expression
  • 2.4 Preparation of Fixed Samples
  • 2.5 Image Acquisition
  • 2.6 Image Processing and Analysis (Fig. 2)
  • 1.1 Zebrafish Embryos
  • 1.2 Microscope Supplies and Components
  • 1.3 Buffers, Other Reagents, and Tools for Sample Preparation
  • 2.2 Alkaline Phosphatase Staining for 3dpf Embryos
  • 2.1 Microdye and Microresin Injection
  • 3.1 Microangiography
  • 3.2 Imaging Blood and Lymphatic Vessels in Transgenic Zebrafish
  • 1.1 Speed
  • 1.2 Phototoxicity
  • 1.3 Resolution and Image Quality
  • 1.4 Realization
  • 1.5 Components of a Selective Plane Illumination Microscopy
  • 2.1 Specimen Size Versus Field of View and Movement Range of the Stages: ("Does Your Sample Fit Into the Microscope?")
  • 2.3 Desired Spatial and Temporal Resolution: ("Can I Actually Resolve the Objects I Want to Detect and Can I Image Fast/Long En ...
  • 3.1 Confined Primed Conversion Enables Photoconversion of Single Cells In Vivo
  • 3.2 Confined Primed Conversion of Neurons Is a Powerful Tool for Neural Morphology Analysis
  • 2.4 Mounting of the Sample: ("Is There a Reliable Routine to Mount the Specimen of Choice?")
  • 1.1 PED6
  • 1.2 NBD- and BODIPY-Cholesterol
  • 2.1 Method 1: Detection of Ciliary Proteins Using Immunohistochemistry
  • 2.1 BODIPY (Excitation/Emission Maxima ~503/512nm)
  • 2.2 BODIPY C2
  • 2.3 BODIPY C5
  • 1.1 Kupffer's Vesicle
  • 1.2 The Pronephros
  • 1.3 Sensory Organs
  • 3.1 Method 6: Evaluation of Heart Position in Live Embryos
  • 3.2 Method 7: Evaluation of Kidney Function
  • 3.3 Method 8: Analysis of Sensory Cell Morphology
  • 2.2 Method 2: Live Imaging of Cilia and Basal Bodies Using Transgenic Lines
  • 2.3 Method 3: Live Imaging of Cilia Movement
  • 2.4 Method 4: Live Imaging of Cilia Using Light Sheet Microscopy
  • 2.5 Method 5: Analysis of Ciliary Transport Using Inducible Transgenes
  • 3.4 Method 9: Staining of Neuromast Hair Cells in Live Specimen
  • 3.5 Method 10: Labeling of Olfactory Neurons by DiI Incorporation
  • 1.1 Selecting a Calcium Indicator
  • 1.2 Comparison of Calcium Indicators
  • 1.3 Validating a Relevant Calcium Signal
  • 3.1 Image Registration Using ImageJ
  • 3.2 Signal Detection and Representation in ImageJ
  • 3.3 Spatial Detection and Visualization Using MATLAB
  • 2.1 General Microscope and Equipment Requirements
  • 2.2 Choosing a Specific Imaging System
  • 2.3 Determining Optimal Imaging Parameters
  • 2.4 Synchronizing a Stimulus With Image Acquisition
  • 1.1 Ethics Statement
  • 1.2 Larval Tissue Preparation
  • 1.4 Recording Chamber and Larval Mounting
  • 1.5 Physiological Solutions
  • 2.1 Mechanical Stimulation
  • 2.2 Optical Stimulation
  • 5.1 Action Current Electrodes and Placement
  • 5.2 Establishing an Action Current Recording
  • 5.3 Analysis of Afferent Fiber Spiking
  • 4.1 Identification and Access
  • 4.2 Electrophysiology Electrodes and Placement
  • 4.3 Establishing Whole-Cell Recordings
  • 4.4 Analysis of Whole-Cell Recording
  • 3.1 Microphonics Equipment and Setup
  • 3.2 Positioning the Microphonic Recording Electrode
  • 3.3 Recording Microphonic Potentials
  • 3.4 Analysis of Microphonic Potentials
  • 1.4 Spinal Canal
  • 3.3.1 Photoreceptor cells
  • 3.3.2 Auditory system hair cells
  • 3.3.3 Lateral line hair cells
  • 1.3.1 Photoreceptors
  • 1.3.2 Mechanosensory hair cells
  • 1.3.3 Olfactory sensory neurons
  • 2.1.1 Method 1a: staining of whole embryos at 3dpf and younger
  • 2.1.2 Method 1b: staining of whole larvae at 5-7dpf
  • 2.1.4 Method 1d: staining of cryosections (all stages)
  • 2.3.1 Spatial resolution
  • 3.2.2 Short-term mounting for time-lapse imaging
  • 3.2.4 Imaging the zebrafish vasculature using light sheet microscopy
  • 3.2.5 Imaging the zebrafish vasculature using superresolution microscopy
  • 3.2.1 Long-term mounting for time-lapse imaging
  • 3.1.1 Materials
  • 2.1.1 Resin injection method
  • 2.2.1 Materials
  • 2.2.2 Protocol
  • 2.1.2 Dye injection method
  • 4.1.1 Generation of ZKS cells
  • 4.1.3 Generation of ZEST cells
  • 4.1.4 Maintenance and culture of ZEST cells
  • 4.1.5 Protocols for in vitro proliferation and differentiation assays on ZKS and ZEST cells
  • 4.2.1 Methylcellulose
  • 4.2.4 Enumeration of colony forming units
  • 1.2.1 Overview
  • 1.2.2 Materials and reagents
  • 1.2.3 Plating zebrafish neural crest cells
  • 1.2.4 Representative results
  • 2.2.1 Protocols for isolating hematopoietic cells from adult zebrafish
  • 2.2.2 Transplanting WKM
  • 2.2.5 Transplantation
  • 2.1.1 Protocol for isolating hematopoietic cells from embryos
  • 2.1.2 Transplanting purified cells into embryonic recipients
  • 2.1.4 Transplanting cells into 48hpf embryos
  • 1.1.1 Overview
  • 1.1.2 Materials and reagents
  • 1.1.4 Representative results
  • 2.1.2.1 Materials
  • 2.1.1.1 Materials
  • 3.1.2.1 Preparation of the apparatus
  • 3.1.2.2 Experimental procedure
  • 3.2.1.1 Materials
  • 3.2.1.2.1 Preparation of imaging chambers
  • 3.2.1.2.2 Mounting animals in imaging chambers
  • 2.1.1.2.1 Preparation of the apparatus
  • 2.1.1.2.2 Experimental procedure
  • 2.1.2.2.1 Dye injection of embryos and early larvae
  • 2.1.2.2.2 Dye injection of juvenile and adult zebrafish

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