Fluorescence imaging, at macro, micro, and submicro scales, has revolutionized biological science in the past 30 years. Immunolabelling has provided precise targeting of molecules in fixed tissue, while fluorescent proteins have enabled localization in living tissues. Fluorescent indicators enable imaging of dynamic changes in cell metabolism.
This book covers, for the first time, imaging at all scales from macro to submicro (superresolution). Its authors include Robert Clegg, legendary teacher and researcher (who, sadly, passed away during the editing); Jim Pawley, editor of several editions of the Handbook of Biological Confocal Microscopy; the famous and now dispersed New Zealand team of Mark Cannell, Christian Soeller, and David Baddeley; Robert Hoffman, pioneer of whole-animal imaging in cancer research; Andreas Schoenle and Christian Eggeling on STED nanoscopy, and many more famous participants in this field. All the contributors are at the cutting edge of their field.
Guy Cox is honorary associate professor at the School of Medical Science and Australian Centre for Microscopy & Microanalysis, University of Sydney, Australia. He has been and is a member of several learned and professional societies, including the Australasian Society for Phycology and Aquatic Botany, Australian Microscopy and Microanalysis Society, Australian Speleological Federation, Phycological Society of America, USA, and Royal Microscopical Society, UK. Prof. Cox is also on the editorial board of Micron.
What Is Fluorescence?
The Ploem Fluorescence Microscope
The Confocal Fluorescence Microscope
Stephen Cody and Guy Cox
Multiphoton Fluorescence Microscopy
Mark Cannell, Guy Cox, and Warren Zipfel
Dyes for Labeling Organelles and Compartments
Indicator and Reporter Dyes
New Approaches to Cancer Therapy Using In Vivo Fluorescent- Protein Imaging
Fluorescence Lifetime Imaging
Chittanon Buranachai, John P. Eichorst, Kei Wei Teng, and Robert M. Clegg
Dynamic Experiments: FRAP and Photoconversion
Kelly Rogers and Sarah Ellis
Förster Resonant Energy Transfer (FRET)
Vinod Jyothikumar, Yuansheng Sun, and Ammasi Periasamy
Recording the Fluorescent Image
Practical Aspects of Localisation Microscopy
Mark Cannell, Christian Soeller, and David Baddeley
Super-Resolution Optical Microscopy with Structured Illumination
Trevor Smith and Liisa Hirvonen
Christian Wurm, Andreas Schoenle, and Christian Eggeling