Stem Cell Niche

Methods and Protocols
Humana (Verlag)
  • 2. Auflage
  • |
  • erschienen am 21. Juni 2020
  • Buch
  • |
  • Softcover
  • |
  • XII, 199 Seiten
978-1-4939-9510-3 (ISBN)
This updated collection addresses the intense efforts that have been underway to characterize the cellular-molecular-biochemical elements of not only the hematopoietic stem cell niche but the niches for other stem cells. These specialized microenvironments that regulate stem cell function have continued to inspire tremendous interest as a subject of study in the years since the publication of the first edition. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Stem Cell Niche: Methods and Protocols, Second Edition serves as an ideal guide for both experts and novices in the stem cell field.
2nd ed. 2019
  • Englisch
  • NY
  • |
  • USA
Springer US
  • 29
  • |
  • 6 s/w Abbildungen, 28 farbige Tabellen, 29 farbige Abbildungen
  • Höhe: 25.4 cm
  • |
  • Breite: 17.8 cm
978-1-4939-9510-3 (9781493995103)
weitere Ausgaben werden ermittelt

1. In Vitro Maintenance of Multipotent Neural Crest Stem Cells as Crestospheres

Sofie Mohlin and Laura Kerosuo

2. Analysis of Hematopoietic Niche in the Mouse Embryo

Keai Sinn Tan, Nathalie Brouard, and Daisuke Sugiyama

3. Functional Assays of Stem Cell Properties Derived from Different Niches

Beatriz de Lucas, Laura M. Pérez, and Beatriz G. Gálvez

4. Ex Vivo Visualization and Analysis of the Muscle Stem Cell Niche

Aviva J. Goel and Robert S. Krauss

5. Stem Cell-Derived Cardiac Spheroids as 3D In Vitro Models of the Human Heart Microenvironment

Madeline Campbell, Mamta Chabria, Gemma A. Figtree, Liudmila Polonchuk, and Carmine Gentile

6. Isolation, Propagation, and Clonogenicity of Intestinal Stem Cells

Prashanthi Ramesh, Aleksandar Buryanov Kirov, David Johannes Huels, and Jan Paul Medema

7. Isolation of Extracellular Vesicles from Subventricular Zone Neural Stem Cells

Mary C. Morton, Victoria N. Neckles, and David M. Feliciano

8. Reconstruction of Regenerative Stem Cell Niche by Cell Aggregate Engineering

Bing-Dong Sui, Bin Zhu, Cheng-Hu Hu, Pan Zhao, and Yan Jin

9. Three-Dimensional Co-Culture of Human Hematopoietic Stem/Progenitor Cells and Mesenchymal Stem/Stromal Cells in a Biomimetic Hematopoietic Niche Microenvironment

Marta H.G. Costa, Tiago S. Monteiro, Susana Cardoso, Joaquim M.S. Cabral, Frederico Castelo Ferreira, and Cláudia L. da Silva

10. Investigating the Vascular Niche: Three-Dimensional Co-Culture of Human Skeletal Muscle Stem Cells and Endothelial Cells

Claire Latroche, Michèle Weiss-Gayet, and Bénédicte Chazaud

11. Identification and Characterization of Stem Cells in Oral Cancer

Sujit K. Bhutia, Prajna P. Naik, Prakash P. Praharaj, Debasna P. Panigrahi, Chandra S. Bhol, Kewal K. Mahapatra, Sarbari Saha, and Srimanta Patra

12. Determining Competitive Potential of Bone Metastatic Cancer Cells in the Murine Hematopoietic Stem Cell Niche

Sun H. Park, Matthew R. Eber, Russell S. Taichman, and Yusuke Shiozawa

13. Spatial Genomic Analysis: A Multiplexed Transcriptional Profiling Method that Reveals Subpopulations of Cells within Intact Tissues

Antti Lignell and Laura Kerosuo

14. 3D Culture of Mesenchymal Stem Cells in Alginate Hydrogels

Sílvia J. Bidarra and Cristina C. Barrias

15. Isolation and Identification of Murine Bone Marrow-Derived Macrophages and Osteomacs from Neonatal and Adult Mice

Joydeep Ghosh, Safa F. Mohamad, and Edward F. Srour

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