Polypeptide Materials: Methods and Protocols

Methods and Protocols
 
 
Springer (Verlag)
  • erscheint ca. am 19. September 2020
 
  • Buch
  • |
  • Hardcover
  • |
  • 286 Seiten
978-1-0716-0927-9 (ISBN)
 
This book details the synthesis and assembly of polypeptide materials across length scales, i.e. proteins and peptides, their precursors, conjugates, and derivatives. A particular emphasis is made on measurement tools and procedures for material characterization, both physicochemical and functional. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Polypeptide Materials: Methods and Protocols serves to reflect the inter-disciplinary nature of molecular biology as well as the importance of developing innovative measurement methods to advance this vital area of research.
2020
  • Englisch
  • New York, NY
  • |
  • USA
  • Für Beruf und Forschung
  • 68 farbige Tabellen, 68 farbige Abbildungen, 15 s/w Abbildungen
  • |
  • 68 Tables, color; 68 Illustrations, color; 14 Illustrations, black and white; Approx. 270 p. 82 illus., 68 illus. in color.
  • Höhe: 254 mm
  • |
  • Breite: 178 mm
978-1-0716-0927-9 (9781071609279)
10.1007/978-1-0716-0928-6
weitere Ausgaben werden ermittelt

1. A Shortcut to the Synthesis of Peptide Thioesters Using an HF-Free Approach

Richard Raz and John Offer

2. SnoopLigase-Mediated Peptide-Peptide Conjugation and Purification

Can M. Buldun, Irsyad N.A. Khairil Anuar, and Mark Howarth

3. Peptide Nanoparticles for Gene Packaging and Intracellular Delivery

Paula V. Gomez, James E. Noble, and Maxim G. Ryadnov

4. Synthesis and Application of Peptide-siRNA Nanoparticles from Disulfide-Constrained Cyclic Amphipathic Peptides for the Functional Delivery of Therapeutic Oligonucleotides to the Lung

Jade J. Welch, David A. Dean, and Bradley L. Nilsson

5. FRET-Mediated Observation of Protein-Triggered Conformational Changes in DNA Nanostructures

Simon Chi-Chin Shiu, Yusuke Sakai, Julian A. Tanner, and Jonathan G. Heddle

6. Molecular Simulations Guidelines for Biological Nanomaterials: From Peptides to Membranes

Irene Marzuoli and Franca Fraternali

7. Functional Peptide Nanocapsules Self-Assembled from b-Annulus Peptides

Hiroshi Inaba and Kazunori Matsuura

8. Electrostatic Self-Assembly of Protein Cage Arrays

Soumyananda Chakraborti, Antti Korpi, Jonathan G. Heddle, and Mauri A. Kostiainen

9. Design and Generation of Self-Assembling Peptide Virus-Like Particles with Intrinsic GPCR Inhibitory Activity

Sergey G. Tarasov, Marzena Dyba, Joshua Yu, and Nadya Tarasova

10. Imaging and 3D Reconstruction of De Novo Peptide Capsids

Emiliana De Santis and Maxim G. Ryadnov

11. Monitoring the Assembly and Aggregation of Polypeptide Materials by Time-Resolved Emission Spectra

Abeer Alghamdi, Li Hung C. Chung, and Olaf Rolinski

12. Forming Low Molecular Weight Hydrogels by Electrochemical Methods

Emily R. Cross, Kate McAulay, and Dave J. Adams

13. In Situ Measurements of Polypeptide Samples by Dynamic Light Scattering: Membrane Proteins, a Case Study

Tristan O.C. Kwan, Rosana Inácio dos Reis, and Isabel Moraes

14. Measurement of Peptide Coating Thickness and Chemical Composition Using XPS

David J. H. Cant, Alexander G. Shard, and Caterina Minelli

15. Imaging the Effects of Peptide Materials on Phospholipid Membranes by Atomic Force Microscopy

Katharine Hammond, Georgina Benn, Isabel Bennett, Edward S. Parsons, Maxim G. Ryadnov, Bart W. Hoogenboom, and Alice L.B. Pyne

16. Microfluidic Single-Cell Phenotyping of the Activity of Peptide-Based Antimicrobials

Jehangir Cama and Stefano Pagliara

17. Ultramicrotomy Analysis of Peptide-Treated Cells

Stephanie Rey, Nilofar Faruqui, and Maxim G. Ryadnov

This book details the synthesis and assembly of polypeptide materials across length scales, i.e. proteins and peptides, their precursors, conjugates, and derivatives. A particular emphasis is made on measurement tools and procedures for material characterization, both physicochemical and functional. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Polypeptide Materials: Methods and Protocols serves to reflect the inter-disciplinary nature of molecular biology as well as the importance of developing innovative measurement methods to advance this vital area of research.

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