Table of Contents
A roadmap for plant genome editing.
A perspective from Europe and beyond.
3 July 2023
Editors:
Agnès Ricroch1*, Dennis Eriksson2, Dragana Miladinovic3, Jeremy Sweet4, Katrijn Van Laere5, and Ewa Wozniak-Gientka6
*agnes.ricroch@universite-paris-saclay.fr
1Université Paris-Saclay. Faculté Jean-Monnet. Laboratoire Idest. Sceaux. France
2Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden
3Institute of Field and Vegetable Crops, National Institute of Republic of Serbia, Novi Sad, Serbia
4 Sweet Environmental Consultants, Cambridge, UK
5Flanders Research Institute for Agriculture, Fisheries and Food, Melle, Belgium
6 Institute of Bioorganic Chemistry Polish Academy of Sciences, Poznan, Poland
Publisher: Springer Nature
Section 0- Editorial
Agnès Ricroch1*, Dennis Eriksson2, Dragana Miladinovic3, Jeremy Sweet4, Katrijn Van Laere5, and Ewa Wozniak-Gientka6
*agnes.ricroch@universite-paris-saclay.fr
1Université Paris-Saclay. Faculté Jean-Monnet. Laboratoire Idest. Sceaux. France
2Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden
3Institute of Field and Vegetable Crops, National Institute of Republic of Serbia, Novi Sad, Serbia
4 Sweet Environmental Consultants, Cambridge, UK
5Flanders Research Institute for Agriculture, Fisheries and Food, Melle, Belgium
6Institute of Bioorganic Chemistry Polish Academy of Sciences, Poznan, Poland
Section 1- Plant breeding and Technological Advances
Chapter 1 Genome editing of gene families for crop improvement
Utku Avci utkuavci@gmail.com
and
Hülya Sipahi
Eskisehir Osmangazi University, Faculty of Agriculture, Agricultural Biotechnology Department, 26160 Eskisehir, Türkiye
Chapter 2 Base editing and Prime editing
Hilal Betül Kaya hbkaya@gmail.com hilalbetul.kaya@cbu.edu.tr
Department of Bioengineering, Manisa Celal Bayar University, Manisa, Türkiye
Chapter 3 Novel delivery methods for CRISPR-based plant genome editing
+ Figure
Barbara Doyle Prestwich
School of Biological Earth and Environmental Science, University College Cork, Ireland
Teodoro Cardi
CNR-IBBR. Institute of Biosciences and Bioresources. via Università 133. 80055 Portici. ITALY
Allah Bakhsh
Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
Alessandro Nicolia
Council for Agricultural Research and Economics, Research centre for Vegetable and Ornamental Crops, Via dei Cavalleggeri, 25. 84098 Pontecagnano (SA) - Italy
and Kaushal K. Bhati corresponding kaushalkbhati@gmail.com Fkaushal.bhati@uclouvin.be Louvain Institute of Biomolecular Science, University of Louvain, Louvain la Neuve, Belgium
Chapter 4 Balancing trait improvement with tradeoff side-effects using genome editing technology
Julia Wind julia.wind@keygene.com
Keygene N.V., P.O. Box 216. 6700 AE Wageningen. The Netherlands
Chapter 5 CRISPR/Cas mutation screening: from mutant allele detection to prediction of protein coding potential
+ 3 figures
Elke Vereecke
1,2
*
corresponding
elke.vereecke@ilvo.vlaanderen.be
and
Katrijn Van Laere
3, Tom Ruttink 3
1Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Belgium
2VIB Center for Plant Systems Biology, BE
3Department of Plant Biotechnology and Bioinformatics, Ghent University, BE
Chapter 6 Methods and techniques to select efficient and specific guides for CRISPR-mediated genome editing in plants
Fabio D'Orso1*
corresponding: fabio.dorso@crea.gov.it
and Valentina Forte1, Simona Baima1, Marco Possenti1, Daniela Palma1, Giorgio Morelli1
1CREA - Research Centre for Genomics and Bioinformatics, Rome, Italy
Section 2- Applications of Genome Editing
Chapter 7
Genome editing of a macroalgae with possible global impacts
Hilde-Gunn Opsahl-Sorteberg*, corresponding hilde-gunn.sorteberg@nmbu.no
and Espen Evju
Norwegian University of Life Sciences (NMBU), Faculty of Biosciences, Ås, Norway
Chapter 8 A Short Review of Advances in Plant-Based Antigen Production Strategies and the Production of Viral Vaccine Antigens Derived from CRISPR/Cas9 Genome Edited Nicotiana benthamiana Plants for Enhanced Vaccine Efficacy
Espen Evju* corresponding espen.evju@nmbu.no
and Hilde-Gunn Opsahl-Sorteberg
Norwegian University of Life Sciences (NMBU), Faculty of Biosciences, Ås, Norway
Chapter 9
Precise gene editing of cereals using CRISPR/Cas technology
+ Figure
Pouneh Pouramini
and Goetz Hensel corresponding goetz.hensel@hhu.de
Heinrich-Heine-University, Germany
Chapter 10 Implementing genome editing in barley breeding
Liina Jakobson1*, Corresponding liina.jakobson@metk.agri.ee
Plant Biotechnology Department, Centre of Estonian Rural Research and Knowledge, M. Pilli haru 1, Jõgeva, Estonia,
Signem Oney Birol2, Department of Molecular Biology and Genetics, Faculty of Science, Burdur Mehmet Akif Ersoy University, Burdur, Turkiye
and Ljudmilla Timofejeva3
Plant Biotechnology Department, Centre of Estonian Rural Research and Knowledge, M. Pilli haru 1, Jõgeva, Estonia
Chapter 11
Current status and future prospective of genome editing application in maize
Serena Varotto serena.varotto@unipd.it
Department of Agronomy Food Natural Resources Animal and Environment DAFNAE. University of Padova - Agripolis Viale dell'Università, 16. 35020 Legnaro (PD) Italy
Chapter 12 Using gene editing strategies for wheat improvement + diagram
Domenica Nigro
1
University of Bari Aldo Moro, Department of Soil, Plant and Food Sciences, Via G. Amendola 165/A, 70126, Bari, Italy.
Mark Smedley 2
John Innes Centre, Department of Crop Genetics, Norwich Research Park, Norwich, Norfolk, NR4 7UH, UK
Francesco Camerlango 3
Alma Mater Studiorum, University of Bologna, Department of Agricultural and Food Sciences, Viale Fanin 46, 40127, Bologna, Italy
and
Sadiye Hayta 2* corresponding Sadiye.hayta@jic.ac.uk
Chapter 13
Gene Editing of Wheat to Reduce Coeliac Disease Epitopes in Gluten
MJM Rene Smulders* corresponding: rene.smulders@wur.nl
LJWJ Gilissen, M Juranic, JG Schaart, and CCM van de Wiel
Plant Breeding, Wageningen University & Research, Wageningen, The Netherlands
Chapter 14 Genome Editing in Horticultural Plants: Present Applications and Future Perspective
Nasser Mahna1* and Shahnoush Nayeri2
1 *Corresponding mahna@tabrizu.ac.ir
Department of Horticultural Sciences, Faculty of Agriculture, University of Tabriz, Tabriz-51666, Iran.
2 Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
Chapter
15
Application of CRISPR/Cas-mediated genome editing techniques in leguminous crops
Debajit Das
and Sumita Acharjee* Corresponding sumita.aus@gmail.com
Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, Assam 785013, India
Chapter 16
Genetic improvement in leguminous crops through genome editing
Aida Dervishi
1, Rabia Riaz
2, Muhammad Waqas Choudhry
2, and Allah Bakhsh
2* 1 Department of Biotechnology, Faculty of Natural Sciences, University of Tirana, Albania
2 National Centre of Excellence in Molecular Biology, University of Punjab, Lahore, Pakistan * Corresponding author: allahbaksh@cemb.edu.pk Chapter 17 Soybean improvement and the role of gene editing Nihal Oztolan Erol nihal.oztolan@sabanciuniv.edu Üniversite Cd. No:27 Orta Mh. 34956 Tuzla, Istanbul, Turkey Chapter 18 Oilseed rape (
Brassica napus L.) CRISPR/Cas-based precision breeding - recent improvements Justyna Boniecka jboniecka@umk.plKatedra Genetyki, Wydzial Nauk Biologicznych i Weterynaryjnych, Uniwersytet Mikolaja Kopernika, ul. Lwowska 1, 87-100 Torun, Poland Center for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Torun, Wilenska 4, 87-100, Torun, Poland
Chapter 19
Targeted gene editing in pome fruit genetics and breeding: state-of-the-art, application potential and perspectives
Hanne Claessen1, Pollien Aert1 and Nico De Storme1,2*
1Laboratory for Plant genetics and Crop Improvement (PGCI), Division of Crop Biotechnics, Department of Biosystems, University of Leuven, 3000, Leuven, Belgium
2Leuven Plant Institute (LPI), KU Leuven, 3001 Leuven, Belgium
*Corresponding nico.destorme@kuleuven.be
Chapter 20
Genome editing in forest trees
Tobias Bruegmann*, Alexander Fendel**, Virginia Zahn**, Matthias Fladung
* Corresponding: tobias.bruegmann@thuenen.de
Thünen Institute of Forest Genetics, Sieker Landstrasse 2, D-22927 Grosshansdorf, Germany;
**) equal contribution.
Chapter 21 Genome editing for reduction of bitterness and for production of medicinal terpenes in Cichorium species
+ 1 Fig
Katarina Cankar1*, Corresponding katarina.cankar@wur.nl
Katrijn Van Laere2, and Dirk Bosch1
1Wageningen University & Research, Wageningen Plant Research, NL
2Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), BE
Chapter 22 Engineering phytonutrient content of tomato by genome editing
technologies
Aurelia Scarano
and corresponding: Angelo Santino angelo.santino@ispa.cnr.it
Institute of Science of Food Production, C.N.R., Unit of Lecce, via Monteroni, 73100, Lecce. Italy
Chapter 23
Breeding for yield quality parameters and abiotic stress in tomato using genome editing
Pedro García-Caparrós pedrogar123@hotmail.com
Agronomy Department of Superior School Engineering. University of Almeria, Almeria, Spain
Chapter 24
Genome Editing-Based Strategies Used to Enhance Crop Resistance to Parasitic Weeds
Kubilay Yıldırım1* Corresponding: kubilay.yildirim@omu.edu.tr
Musa Kavas2, Meleksen Akın3, and Ilkay Sevgen Küçük1
1 Department of Molecular Biology and Genetics, Ondokuz Mayıs University, Samsun, Türkiye
2 Department of Agricultural Biotechnology, Ondokuz Mayıs University, Samsun, Türkiye
3Department of Horticulture, Igdır University, Igdır, Türkiye
Section 3- Policies and Regulations
Chapter 25 Genome editing in biotech regulations worldwide
+ 1 Fig
Thorben Sprink
and Ralf Wilhelm* ralf.wilhelm@julius-kuehn.de
Julius Kühn-Institut. Institute for Biosafety in Plant Biotechnology. Erwin-Baur-Straße 27, D-06484 Quedlinburg. Germany
Chapter 26 Interpreting Precision Breeding: Key legal concepts under international law and current domestic regulatory approaches in the Global South
André Rosado ayrtonandre.rosadohuaynasi@student.kuleuven.be
Faculty of Science, KU Leuven. Leuven, Belgium.
Centro de Investigaciones de Zonas Aridas, Universidad Nacional Agraria La Molina. Lima, Peru
Chapter 27 CRISPR Processes Patents in Green Biotechnology: Collaborative Licensing Models
Agnès Ricroch agnes.ricroch@universite-paris-saclay.fr
Université Paris-Saclay. Faculté Jean-Monnet. Laboratoire Idest. Sceaux. France
Section 4- Public and stakeholder perceptions
Chapter 28
The view of the European seed sector on genome editing tools in plant breeding
+ 3 Fig
Petra
Jorasch* Corresponding: PetraJorasch@euroseeds.eu
and
Nick Vangheluwe
EuroSeeds, Avenue des Arts 52, Brussels. Belgium
Chapter
29
The awareness of the Polish society on new genomic techniques
Wiktoria Molodziejko,1 Justyna Nowakowska
2,
and Anna Linkiewicz
2,*
*corresponding a.linkiewicz@uksw.edu.pl
1 Faculty of Biology and Environmental Sciences, Cardinal Stefan Wyszynski University in Warsaw, Wóycickiego 1/3, 01-938 Warsaw, Poland
2 Laboratory of Molecular Biology and Genetics, Institute of Biological Sciences, Cardinal Stefan Wyszynski University in Warsaw, Wóycickiego 1/3, 01-938 Warsaw, Poland
Chapter 30
Improving Science Communication about Genome Editing - Mitigating Strong Moral Convictions through Shared Moral Goals
Gabi Waldhof waldhof.gabriela@gmail.com
Faculty of Business, Economics and Social Sciences, Department of Socioeconomics, Germany
Chapter 31
The citizens' awareness and concerns during the transition from Genetically Modified to Genome Edited plants in Europe about their use in agriculture and food production
Mihael-Cristin Ichim cichim@hotmail.com
"Stejarul" Research Centre for Biological Sciences, National Institute of Research and Development for Biological Sciences, Piatra Neamt, Romania
Chapter 32
What is the problem with Europe in a philosophical point of view?
Marcel Kuntz
mrk683830@gmail.com
Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes, CNRS, CEA, INRAE, Grenoble, France
Chapter 33
NGT plant products in the EU. The postulates, the outlooks, and possible consequences of a regulatory system reform in the context of legislative reforms in third countries and detection requirements
Tomasz Zimny tzimny@inp.pan.pl
Institute of Law Studies, Polish Academy of Sciences Department of Polish and European Industrial Property Law. Nowy Swiat St. 72. 00-330 Warsaw. Poland
Section 5-
Future outlook
Agnès Ricroch1*, Dennis Eriksson2, Dragana Miladinovic3, Jeremy Sweet4, Katrijn Van Laere5, and Ewa Wozniak-Gientka6
*agnes.ricroch@universite-paris-saclay.fr
1Université Paris-Saclay. Faculté Jean-Monnet. Laboratoire Idest. Sceaux. France
2Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden
3Institute of Field and Vegetable Crops, National Institute of Republic of Serbia, Novi Sad, Serbia
4 Sweet Environmental Consultants, Cambridge, UK
5Flanders Research Institute for Agriculture, Fisheries and Food, Melle, Belgium
6 Institute of Bioorganic Chemistry Polish Academy of Sciences, Poznan, Poland