Microbial Transposon Mutagenesis

Protocols and Applications
 
 
Humana (Verlag)
  • erschienen am 28. Juni 2020
 
  • Buch
  • |
  • Softcover
  • |
  • XI, 183 Seiten
978-1-4939-9572-1 (ISBN)
 
This book addresses cutting-edge techniques for researching transposon mutagenesis, an approach for identifying individual gene contributions to the phenotypic characteristics of a particular microorganism. The volume begins with methods for specific microorganisms and include protocols for individual microorganisms ranging from pathogens such as Salmonella to Bifidobacterium, a microorganism considered beneficial to humans and animals. The final section addresses more general protocols including plasmid transfer and bioinformatic tools as well as novel applications of transposon methodologies such as transposon-aided capture of antibiotic resistant plasmids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Microbial Transposon Mutagenesis: Protocols and Applications serves as a valuable reference for scientists seeking to apply transposon mutagenesis to microbial genetic analyses and functionality.
1st ed. 2019
  • Englisch
  • NY
  • |
  • USA
Springer US
  • 34
  • |
  • 12 s/w Abbildungen, 34 farbige Tabellen, 34 farbige Abbildungen
  • Höhe: 25.4 cm
  • |
  • Breite: 17.8 cm
978-1-4939-9572-1 (9781493995721)
10.1007/978-1-4939-9570-7
weitere Ausgaben werden ermittelt

Part I: Transposon Protocols for Specific Microorganisms

1. Preparation of Transposon Library and Tn-Seq Amplicon Library for Salmonella Typhimurium

Sardar Karash, Tieshan Jiang, Deepti Samarth, Reena Chandrashekar, and

Young Min Kwon

2. Application of Whole Genome Sequencing to Transposon Mutants of Salmonella Heidelberg

Bryna Rackerby, Sang In Lee, Ian Moppert, Steven C. Ricke, KwangCheol C. Jeong, and Si Hong Park

3. Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus

Jennifer L. Endres, Vijaya Kumar Yajjala, Paul D. Fey, and Kenneth W. Bayles

4. Transposon Mutagenesis in Streptococcus Species

Martin Nilsson, Michael Givskov, and Tim Tolker-Nielsen

5. Implementation of Transposon Mutagenesis in Bifidobacterium

Lorena Ruiz and Douwe van Sinderen

6. Transposon Mutagenesis of Listeria monocytogenes

Oindrila Paul, Damayanti Chakravarty, and Janet R. Donaldson

7. Transposon Mutagenesis of Foodborne Pathogenic Esherichia coli

Supraja Puttamreddy and F. Chris Minion

8. Methods for Transposon Mutagenesis in Proteus mirabilis

Philip N. Rather

9. Mutagenesis of Vibrio fischeri and Other Marine Bacteria Using Hyperactive Mini-Tn5 Derivatives

Julie L. Stoudenmire, Michael Black, Pat M. Fidopiastis, and Eric V. Stabb

10. Transposon Mutagenesis of Bacteroides fragilis

Yaligara Veeranagouda, Fasahath Husain, and Hannah M. Wexler

11. Transposon Mutagenesis in Mycobacterium avium Subspecies Paratuberculosis

John P. Bannantine, Denise K. Zinniel, and Raúl G. Barletta

Part II: Transposon General Protocols and Applications

12. Protocols of Conjugative Plasmid Transfer in Salmonella: Plate, Broth, and Filter Mating Approaches

Bijay K. Khajanchi, Pravin R. Kaldhone, and Steven L. Foley

13. Construction of DNA Barcode-Tagged Salmonella Strains

Yichao Yang, Reena Chandrashekar, Steven C. Ricke, and Young Min Kwon

14. Transposon-Aided Capture of Antibiotic Resistance Plasmids from Complex Samples

Sarah Delaney, Richard Murphy, and Fiona Walsh

15. Efficient Gene Deletion Method for Listeria monocytogenes

Hossam Abdelhamed, Attila Karsi, and Mark L. Lawrence

16. Whole Genome Identification and Characterization of Bacterial Insertion Sequences Using Bioinformatic Tools

Kody A. Bassett, Melanie R. Mormile, and Ronald L. Frank


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