Carbonic Anhydrase as Drug Target

Thermodynamics and Structure of Inhibitor Binding
Springer (Verlag)
  • erschienen am 3. Juni 2019
  • Buch
  • |
  • Hardcover
  • |
  • XXVI, 353 Seiten
978-3-030-12778-7 (ISBN)
This book offers deep insights into the thermodynamics and molecular structures of the twelve catalytically active isoforms of human carbonic anhydrase (CA) with a particular focus on inhibitor binding for drug design. X-ray crystallographic structures in combination with enzyme kinetic testing provide information on the interaction of CAs and their inhibitors, knowledge which is crucial for rational drug design. CAs are zinc carrying enzymes that catalyse the reversible interconversion of carbon dioxide and bicarbonate and are involved in numerous cellular processes. They are therefore a common target for drugs. The suppression of CA activities through inhibitory compounds has found application for example in diuretics and in glaucoma therapy. In this book methods used to determine binding thermodynamics of inhibitory compounds (Isothermal titration calorimetry, Fluorescent thermal shift assay/differential scanning fluorimetry and others) will be compared in detail. Also types and chemical synthesis of CA inhibitors, the use of antibodies against CAs as well as inhibitor application in animals are discussed.
1st ed. 2019
  • Englisch
  • Cham
  • |
  • Schweiz
Springer International Publishing
  • Für Beruf und Forschung
  • 107 farbige Abbildungen, 62 s/w Abbildungen, 60 farbige Tabellen
  • |
  • 60 schwarz-weiße und 60 farbige Abbildungen, Bibliographie
  • Höhe: 241 mm
  • |
  • Breite: 162 mm
  • |
  • Dicke: 22 mm
  • 844 gr
978-3-030-12778-7 (9783030127787)
weitere Ausgaben werden ermittelt

Prof. Dr. Daumantas Matulis

Vilnius University

Life Sciences Center

Institute of Biotechnology

Department of Biothermodynamics and Drug Design

Sauletekio 7, 10257 Vilnius


Final ToC tbd. 1) Description of human carbonic anhydrases (12 catalytically active human isoforms; isoforms as drug targets, enzymatic activity, catalytic mechanism, thermodynamics of protonation of the water molecule in the active site).- 2) Thermal stabilities of all isoform CA catalytic domains.- Thermodynamics of inhibitor binding to CAs (incl. intrinsic thermodynamics - not mentioned important subject in most CA literature).- 3) Comparison of methods to determine inhibitor binding to CAs (ITC, FTSA, enzymatic activity assays).- 4) Chemical synthesis of CA inhibitors.- 5) X-ray crystallographic structures of CAs and their complexes with inhibitors.- 6) Antibodies against CAs.

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