Plant Functional Genomics

Food Products Press,U.S.
  • 1. Auflage
  • |
  • erscheint ca. am 23. Dezember 2004
  • Buch
  • |
  • Hardcover
  • |
  • 692 Seiten
978-1-56022-998-8 (ISBN)
Discover cutting-edge knowledge for engineering a more productive and environment-friendly agriculture!

In Plant Functional Genomics, you'll find a cross-section of state-of-the-art research on the biological function of plant genes and how they work together in health and disease. World-leading scientists in the field present breakthrough techniques, discuss the results of projects aimed at dissecting particular plant functions, and provide an overview on the state of functional genomics for several plant and plant-related species. With figures, tables, and illustrations, this book will help scientists, researchers, and advanced students in botany find new ways of creating novel plant forms to better serve the needs of a rapidly expanding human population.

Plant Functional Genomics will increase your understanding of gene networks and systems rules, as well as gene expression during specific conditions or development or treatments. This important resource contains a wealth of data generated by various plant genome sequencing projects, including the newest results from experiments with Arabidopsis thaliana?the first plant to be completely sequenced. This book also contains innovative research on:

- T-DNA mutagenesis

- transcriptomics and metabolic profiling in plants

- large-scale yeast two-hybrid analyses

- the exceptional model system of Chlamydomonas genomics

- functional genomics in rice, maize, and Physcomitrella

- prospects for functional genomics in a new model grass

- chloroplast and plant mitochondrial proteomics

- plant transporters

- so much more
Plant Functional Genomics will help speed up the identification and isolation of genes that might be of interest with respect to diverse biological questions. This valuable contribution to the field clarifies the challenges yet to be faced and the opportunities that could some day expand the frontiers of plant sciences.
  • Englisch
  • Abingdon
  • |
  • Großbritannien
Taylor & Francis Ltd
  • Für Beruf und Forschung
  • 1243 gr
978-1-56022-998-8 (9781560229988)
1560229985 (1560229985)
weitere Ausgaben werden ermittelt
- About the Editor

- Contributors

- Preface. Definition and Origin of Plant Functional Genomics

- Abbreviations


- Chapter 1. Full-Length cDNAs for the Discovery and Annotation of Genes in Arabidopsis thaliana (Motoaki Seki, Mari Narusaka, Masakazu Satou, Miki Fujita, Tetsuya Sakurai, Youko Oono, Kenji Akiyama, Kazuko Yamaguchi-Shinozaki, Kei Iida, Piero Carninci, Junko Ishida, Jun Kawai, Maiko Nakajima, Yoshihide Hayashizaki, Akiko Enju, and Kazuo Shinozaki)

- Introduction

- Collection of Arabidopsis Full-Length cDNAs

- Application of Full-Length cDNAs to Genome Annotation

- Application of Full-Length cDNAs to Functional Analysis

- Conclusions and Perspectives

- Chapter 2. T-DNA Mutagenesis: From Tagging to Insertion Sequence Databases (Allen Sessions)

- Crown Galls, Neoplasia, and Agrobacterium

- Agrobacteria As Friend Not Foe

- Development of Transformation Protocols for the Efficient Generation of T-DNA Insertion Mutants

- Even Faster and More Efficient Transformation

- From a Population of T-DNA Insertion Mutants to a File of T-DNA Flanking Sequence Tags

- Amplification of T-DNA Borders and the Distribution of Predicted Insertions

- Chapter 3. Novel Reverse Genetics Tools in Plant Functional Genomics (Xin Li and Yuelin Zhang)

- Introduction

- Deleteagene


- Combinatorial Gene Slicing

- Chapter 4. Transcriptomics in Plants: From Expression to Gene Function (Laurent Zimmerli and Shauna Somerville)

- Introduction

- The Technologies

- Experimental Design

- Analysis

- Data Verification and Quality Checks

- Limitations

- Perspective

- Chapter 5. Metabolite Profiling in Plants (Richard N. Trethewey)

- Introduction

- Background and Fundamentals of Metabolite Profiling

- Metabolite-Profiling Technologies

- Applications of Metabolite Profiling to Plants

- Outlook for Metabolite Profiling

- Chapter 6. Large-Scale Yeast Two-Hybrid Analysis (Barry Causier, John Graham, and Brendan Davies)

- Introduction

- The Basis of the Yeast Two-Hybrid System

- Large-Scale Yeast Two-Hybrid Studies: A Brief History

- High-Throughput Yeast Two-Hybrid Methodologies

- Applications of Large-Scale Yeast Two-Hybrid Screening

- The Caveat


- Chapter 7. Functional Genomics of a Cyanobacterium, Synechocystis sp. PCC 6803 (Takakazu Kaneko, Shusei Sato, and Satoshi Tabata)

- Introduction

- A Large-Scale Gene Disruption

- Transcriptome Analysis

- Proteome Analysis

- Analysis of Protein Interactions with the Yeast Two-Hybrid System

- Genome Databases

- Conclusions and Future Perspectives

- Chapter 8. Chlamydomonas Genomics: New Approaches for a Classical Model System (Heidi K. Ledford, Rachel M. Dent, and Krishna K. Niyogi)

- Introduction

- The Chloroplast Genome

- The Mitochondrial Genome

- The Nuclear Genome

- Functional Genomics

- Transcriptomics

- Proteomics and Metabolomics

- Tying It All Together: Databases for the Chlamydomonas Community

- Closing Thoughts and Future Directions

- Chapter 9. Functional Genomics in Physcomitrella (Wolfgang Frank, Hauke Holtorf, and Ralf Reski)

- Introduction

- Mass Production of Standardized Moss Material

- Production of a Physcomitrella EST Collection

- In Vivo Transposon Mutagenesis of cDNA Libraries

- Fast-Forward Production of Single Knockout Constructs

- High-Throughput Transforma

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