Part 1 Basic techniques in plant biotechnology: vectors for gene transfer in higher plants; methods for transforming plant cells; techniques in plant cell and tissue culture; selected topics in the genetic manipulation of nuclear genome; regulation and self-expression of plant genes in microorganisms. Part 2 Regulation of gene expression in plants: the molecular architecture of plant genes - a look at the cellular level; gene expression in early embryogenesis; photoregulation of gene expression in plants; hormonal and stress regulation of gene expression in cereal aleurone layers; auxin-regulated gene expression in plants; cytokinin-modulated macromolecular synthesis and expression of genes for photosynthetic and pigments-protein complexes in photosynthetic bacteria. Part 3 Prospects for manipulation of chloroplast genomes: organization and expression of the "Nicotania" chloroplast genome.