Lymphoma

Methods and Protocols
 
 
Humana Press Inc.
  • 2. Auflage
  • |
  • erschienen am 19. Februar 2019
 
  • Buch
  • |
  • Hardcover
  • |
  • XII, 439 Seiten
978-1-4939-9150-1 (ISBN)
 
This second edition volume expands on the previous edition with descriptions of recent developments in the field. The new and updated chapters discuss the latest approaches for studying single cell RNA sequencing, whole genome sequencing data, CRISPR/Cas9-mediated gene knockout, protein interactions by a proximity ligation assay, and detection of circulating cell-free tumor DNA in lymphomas. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Innovative and thorough, Lymphoma: Methods and Protocols, Second Edition is a valuable resource for hematologists, hematopathologists, and any scientists interested in cancer research, human genetics, and immunology.
2nd ed. 2019
  • Englisch
  • NY
  • |
  • USA
  • Für Beruf und Forschung
  • Überarbeitete Ausgabe
  • 47 farbige Tabellen, 18 s/w Abbildungen, 48 farbige Abbildungen
  • |
  • 17 schwarz-weiße und 50 farbige Abbildungen, 47 farbige Tabellen, Bibliographie
  • Höhe: 261 mm
  • |
  • Breite: 181 mm
  • |
  • Dicke: 32 mm
  • 1012 gr
978-1-4939-9150-1 (9781493991501)
10.1007/978-1-4939-9151-8
weitere Ausgaben werden ermittelt
Preface...Table of Contents...Contributing Authors... 1. Origin and Pathogenesis of B Cell LymphomasMarc Seifert, René Scholtysik, and Ralf Küppers 2. Flow Cytometry for Non-Hodgkin and Hodgkin LymphomaEmily Glynn, Lori Soma, David Wu, Brent L. Wood, and Jonathan R. Fromm 3. Laser-Based Microdissection of Single Cells from Tissue Sections and PCR Analysis of Rearranged Immunoglobulin Genes from Isolated Normal and Malignant Human B CellsRalf Küppers, Markus Schneider, and Martin-Leo Hansmann 4. PCR GeneScan and Heteroduplex Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes for Clonality Diagnostics in Suspect Lymphoproliferations Elke Boone, Kim C. Heezen, Patricia JTA Groenen, and Anton W. Langerak 5. Expression Cloning of Antibodies from Single Human B CellsHedda Wardemann and Christian E. Busse 6. Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCRMenno C. van Zelm 7. Stereotyped B Cell Immunoglobulins in B Cell LymphomasAndreas Agathangelidis, Fotis Psomopoulos, and Kostas Stamatopoulos 8. Flow Cytometric MRD Detection in Selected Mature B-Cell MalignanciesSebastian Böttcher 9. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCRChristiane Pott, Monika Brüggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba 10. Generation of Whole Genome Bisulfite Sequencing Libraries from Very Low DNA InputDieter Weichenhan, Charles D. Imbusch, Qi Wang, Benedikt Brors, and Christoph Plass 11. FISH and FICTION in Lymphoma ResearchMaciej Giefing and Reiner Siebert 12. NGS-Based High-Throughput Screen to Identify MicroRNAs Regulating Growth of B-Cell Lymphoma Joost Kluiver, Fubiao Niu, Ye Yuan, Klaas Kok, Anke van den Berg, and Agnieszka Dzikiewicz-Krawczyk 13. RNA Sequencing in B-Cell LymphomasDa Wei Huang, Moez Dawood, Calvin A. Johnson, and Roland Schmitz 14. Studying Cancer Heterogeneity by Single Cell RNA-Sequencing Johannes W. Bagnoli, Lucas E. Wange, Aleksandar Janjic, and Wolfgang Enard 15. Evaluation of Whole Genome Sequencing DataDaniel Huebschmann and Matthias Schlesner 16. Protocols for CRISPR-Cas9 Screening in Lymphoma Cell LinesDaniel E. Webster, Sandrine Roulland, and James Phelan 17. Lymphoma and Leukemia Cell Vulnerabilities and Resistance Identified by Compound Library Screens Katarzyna Tomska, Sebastian Scheinost, and Thorsten Zenz 18. Proximity Ligation AssayRyan M. Young 19. Phospoproteomic Analysis of Signaling Pathways in LymphomasBjörn Häupl, Henning Urlaub, and Thomas Oellerich 20. Ultrasensitive Detection of Circulating Tumour DNA in Lymphoma via Targeted Hybridization Capture and Deep Sequencing of Barcoded LibrariesMiguel Alcaide, Christopher Rushton, and Ryan D. Morin
This second edition volume expands on the previous edition with descriptions of recent developments in the field. The new and updated chapters discuss the latest approaches for studying single cell RNA sequencing, whole genome sequencing data, CRISPR/Cas9-mediated gene knockout, protein interactions by a proximity ligation assay, and detection of circulating cell-free tumor DNA in lymphomas. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Innovative and thorough, Lymphoma: Methods and Protocols, Second Edition is a valuable resource for hematologists, hematopathologists, and any scientists interested in cancer research, human genetics, and immunology.

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