This book discusses unique ion channels and transporters that are located within epithelial tissues of various organs including the kidney, intestine, pancreas and respiratory tract. As the authors show, these channels and transporters play crucial roles in transepithelial ion and fluid transport across epithelia and their contribution to maintaining homeostasis. Readers will be introduced to the fundamentals of ion transport in terms of function, modelling, regulation, structure and pharmacology. This is the first of three volumes highlighting the importance of epithelial ion channels and transporters in basic physiology and pathophysiology of human diseases.
This volume focuses on basic fundamentals of epithelial transport physiology. There is a range of chapters dedicated to specific aspects of epithelial ion transport and cell function. Accordingly, the authors discuss techniques used to determine epithelial function, principles of epithelia transport, polarization of epithelial cells, mathematical modelling of epithelial ion transport, protein folding of ion channels, degradation epithelial ion channels, fundamentals of epithelial sodium, potassium and chloride transport, fundamentals of bicarbonate secretion, volume regulation, and microRNA regulation of epithelial channels and transporters. Given its scope, Volume 1 offers a valuable resource for physiology students, scientists and clinicians alike.
Kirk L. Hamilton was born in Baltimore, Maryland in 1953. He gained his undergraduate (biology/chemistry) and M.Sc. (ecology) degrees from the University of Texas at Arlington. He obtained his Ph.D. at Utah State University under the tutelage of Dr. James A. Gessaman, where he studied incubation physiology of Barn owls. His first post-doctoral position was at the University of Texas Medical Branch in Galveston, Texas under the mentorship of Dr. Douglas C. Eaton where he studied epithelial ion transport, specifically, the epithelial sodium channel (ENaC). He then moved to the Department of Physiology at the University of Alabama, Birmingham for additional post-doctoral training under the supervision of the late Dr. Dale J. Benos where he further studied ENaC, and non-specific cation channels. He took his first academic post in the Department of Biology at Xavier University of Louisiana in New Orleans (1990-1994). He then joined the Department of Physiology at the University of Otago in 1994, and he is currently an Associate Professor. He has focused his research on the molecular physiology and trafficking of potassium channels (specifically KCa3.1). He has published more than 60 papers and book chapters. His research work has been funded by the NIH, American Heart Association, Cystic Fibrosis Foundation, and Lottery Health Board New Zealand. Dr. Devor and he have been collaborators since 1999. When he not working, he enjoys playing guitar (blues and jazz) and volleyball. Kirk is married to Judith Rodda, a recent Ph.D. graduate in spatial ecology. They have 2 children, Nathan (b. 1995) and Emma (b. 1998).
Daniel C. Devor
was born in Vandercook Lake, Michigan in 1961. His education took him through Southampton College of Long Island University, where he studied Marine Biology, before entering SUNY Buffalo for his Ph.D., under the guidance of Dr. Michael E. Duffey. During this time, he studied the role of basolateral potassium channels in regulating transepithelial ion transport. He subsequently did his post-doctoral work at the University of Alabama, Birmingham, under the mentorship of Dr. Raymond A. Frizzell, where he studied both apical CFTR and basolateral KCa3.1 in intestinal and airway epithelia. He joined the University of Pittsburgh faculty in 1995 where he is currently a Professor of Cell Biology. During this time, he has continued to study the regulation, gating and trafficking of KCa3.1 as well as the related family member, KCa2.3, publishing more than 50 papers on these topics. These studies have been funded by the NIH, Cystic Fibrosis Foundation, American Heart Association and pharmaceutical industry. When not in the lab, he enjoys photography and growing exotic plants. Dan is married to Catherine Seluga, an elementary school teacher. They have 3 children, Caitlin (b. 1990), Emily (b. 1993) and Daniel (b. 1997).
1. Techniques in Epithelial Transport.- 2. Understanding Transepithelial Current Measurements.- 3. Mathematical Modeling of Epithelial Ion Transport.- 4. Rationale of Epithelia Transport.- 5. Establishment of Epithelial Polarity.- 6. Molecular Mechanisms of Apical and Basolateral Sorting in Polarized Epithelial Cells.- 7. Membrane Protein Folding and Structure.- 8. Epithelial Ion Channel Folding and ER Associated Degradation (ERAD).- 9. Fundamentals of Epithelial Cl- Transport.- 10. Fundamentals of Epithelial Na+ Absorption.- 11. Physiologic Influences of Transepithelial K+ Secretion.- 12. Volume Regulation in Epithelia.- 13. Fundamentals of Bicarbonate Secretion in Epithelia.- 14. Sexual Dimorphism of Epithelial Ion Channels.- 15. Non-coding RNA-Dependent Regulation of Channels/Transporters.
This book discusses unique ion channels and transporters that are located within epithelial tissues of various organs including the kidney, intestine, pancreas and respiratory tract. As the authors show, these channels and transporters play a crucial role in transepithelial ion and fluid transport across all epithelia and their contribution to maintaining homeostasis. Readers will be introduced to the fundamentals of ion transport in terms of function, modelling, regulation, structure and pharmacology. This is the first of three volumes highlighting the importance of ion channels and transporters in the basic physiology and pathophysiology of human diseases.
The focus of the first volume is on measurement techniques for epithelial transport and the mathematical modelling of these processes. Accordingly, the authors explain e.g. the fundamentals of bicarbonate secretion, volume regulation, protein folding of ion channels and transporters, and fundamentals of epithelial sodium, potassium. and chloride transport, and microRNA regulation of epithelial channels and transporters. Given its scope, the book offers a valuable resource for physiology students, scientists and clinicians alike.