This book provides systematic knowledge of basic principles in the design of fluorescence sensing and imaging techniques together with critical analysis of recent developments. Fluorescence is the most popular technique in chemical and biological sensing because of its ultimate sensitivity, high temporal and spatial resolution and versatility that enables imaging within the living cells. It develops rapidly in the directions of constructing new molecular recognition units, new fluorescence reporters and in improving sensitivity of response up to detection of single molecules. Its application areas range from control of industrial processes to environment monitoring and clinical diagnostics. Being a guide for students and young researchers, it also addresses professionals involved in active basic and applied research. Making a strong link between education, research and product development, this book discusses prospects for future progress.
1. Principles and Techniques in Chemical and Biological Sensing.- 2. Overview of Strategies in Fluorescence Sensing.- 3. Fluorescence Detection in Sensor Technologies.- 4. Photophysical Mechanisms of Signal Transduction in Sensing.- 5. Organic Dyes and Visible Proteins as Fluorescence Reporters.- 6. Small Luminescent Associates based on Inorganic Atoms and Ions.- 7. Fluorescent Inorganic Particles in Nanoscale World.- 8. General Effects Observed on Dye-dye Association.