Molecular Cloning of Staphylococcus Aureus Alpha Hemolysin Toxin Gene

LAP Lambert Academic Publishing
  • erschienen am 16. Januar 2018
  • Buch
  • |
  • Softcover
  • |
  • 128 Seiten
978-620-2-19718-2 (ISBN)
Recombinant technology has crucial impact in therapy development. In microbial environment, Pathogenic bacteria such as Staphylococcus aureus produces alpha hemolysin protein. This protein is used as anticancer protein. alpha hemolysin gene was isolated from pathogenic S. aureus that was isolated from blood samples of patients (chrildren). This recombinant hia gene was produced alpha hemolysin protein by using competent E. coli as a host. The recombinant a- toxin protein was used as anticancer agent.In this study that high concentration of a-toxin (500 µl/ ml) was effected on the cell viability of four cells line ( HepG-2, HCT-116, MCF-7, A-549). The cell viability of cell lines were represented 31.69% in HepG-2, 36.27% in HCT-116, 78.95% in MCF-7, 67.28% in A-549. From our results, Alpha hemolysin protein had high inhibitory activity against HepG-2 and HCT-116, and had weak inhibitory activity against MCF-7 and A-549. Inconclusion,The production of recombinant a- toxin was very simple process, low cost, high efficiency, non-toxic, high yield and no side effect.
  • Englisch
  • Höhe: 220 mm
  • |
  • Breite: 150 mm
  • |
  • Dicke: 8 mm
  • 207 gr
978-620-2-19718-2 (9786202197182)
6202197188 (6202197188)
The AuthorNada Darwesh obtained Bachelor of Science in Botany / Chemistry (2005), Master Degree in Microbiology (2012) and Doctor Degree in Microbiology (2017) from Faculty of Science, Mansoura University, Egypt.

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